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MRPL48 (NM_016055) Human Untagged Clone
CAT＃: SC114521
MRPL48 (untagged)-Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein
CNY 2,400.00
CNY 3,990.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGI-118; HSPC290; L48MT; MRP-L48 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114521 sequence for NM_016055 edited (data generated by NextGen Sequencing)
ATGAGCGGAACCTTGGAAAAGGTGCTGTGCCTGAGGAACAATACCATTTTTAAGCAAGCC TTTTCTCTCTTAAGGTTTAGAACTTCAGGAGAGAAGCCCATCTATTCTGTAGGTGGAATT CTACTAAGTATCAGTCGGCCCTACAAGACAAAGCCCACCCACGGCATTGGAAAGTACAAG CACTTAATTAAAGCAGAAGAGCCCAAGAAGAAGAAGGGAAAAGTGGAAGTGAGAGCCATT AATTTGGGGACAGATTATGAATATGGGGTTTTAAATATTCATCTGACTGCATATGATATG ACCCTGGCAGAGAGTTATGCCCAGTATGTTCACAACCTCTGCAACTCTCTCTCCATTAAA GTCGAGGAAAGTTATGCAATGCCAACCAAAACCATAGAAGTGTTGCAGTTGCAGGACCAA GGCAGCAAAATGCTCCTGGACTCAGTGCTTACCACCCATGAGCGAGTGGTTCAGATCAGC GGTTTGAGTGCTACGTTTGCAGAAATTTTCTTGGAAATAATCCAAAGCAGTCTTCCTGAA GGAGTCAGACTGTCAGTGAAGGAGCACACTGAAGAAGACTTCAAGGGACGATTCAAAGCT CGACCAGAACTGGAAGAACTGTTGGCCAAGTTGAAGTAG Clone variation with respect to NM_016055.5 >OriGene 5' read for NM_016055 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTTCAGACGCCCTGGGA ACGCGGCTGCAGGGTCCGGTCTTCGGTTTGCACAGCTAGAGGCCGCGCAGCAGCAAAGGA TGAGCGGAACCTTGGAAAAGGTGCTGTGCCTGAGGAACAATACCATTTTTAAGCAAGCCT TTTCTCTCTTAAGGTTTAGAACTTCAGGAGAGAAGCCCATCTATTCTGTAGGTGGAATTC TACTAAGTATCAGTCGGCCCTACAAGACAAAGCCCACCCACGGCATTGGAAAGTACAAGC ACTTAATTAAAGCAGAAGAGCCCAAGAAGAAGAAGGGAAAAGTGGAAGTGAGAGCCATTA ATTTGGGGACAGATTATGAATATGGGGTTTTAAATATTCATCTGACTGCATATGATATGA CCCTGGCAGAGAGTTATGCCCAGTATGTTCACAACCTCTGCAACTCTCTCTCCATTAAAG TCGAGGAAAGTTATGCAATGCCAACCAAAACCATAGAAGTGTTGCAGTTGCAGGACCAAG GCAGCAAAATGCTCCTGGACTCAGTGCTTACCACCCATGAGCGAGTGGTTCAGATCAGCG GTTTGAGTGCTACGTTTGCAGAAATTTTCTTGGAAATAATCCAAAGCAGTCTTCCTGAAG GAGTCAGACTGTCAGTGAAGGAGCACACTGAAGAAGACTTCAAGGGACGATTCANAGCTC GACCAGAACTGGAAGAACTGTTGGCCAAGTTGAAGTAGCTACTGGTAGACCCTTTCATGC CAGCAGTGGTCATATTNGAGTGCCAAGAGAAGAGCTACTGGGTAGTTAGAGTTATCAGGA AACCCGACCCTTAATTTCATAGTACCATTCCATAGCAGNATGTCCTACTCTCTGTGGCTG GCTGTACTGCATTNCTACCACTACTATGAGGGAGGCTNGGTATCTCCAC >OriGene 3' read for NM_016055 unedited
GTCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTCTGCAGCAGATTT TTATTAGATGGAAGATAACAAGCATTACCTCATAGGTAAGTGGTAAGAAATGGCAAGTAC AGCCAAGCCACACTAGAGTGAGGACATTACTGGCTATGGGAATGGGTACTTATGAAATCT AAGGGTCGGGTTTCCTGATGAACTCTAACTACCCAGTAAAGCCTCTTTCTCTTTGGCACT CAATATGACCACTGCTGGCATGAAAGGGTCTACAGTAGCTACTTCAACTTGGCCAACAGT TCTTCCAGTTCTGGTCGAACTTTTGATCGTCCCTTGAAGTCTTCTTCAGTGTGCTCCTTC ACTGACAGTCTGACTCCTTCAGGAAGACTGCTTTGGATTATTTCCAAGAAAATTTCTGNC AACGTAGCACTCAAACCGCTGATCTGAAACACTCGCTCATGGGGTGTAAGCACTGAGTCC AGGAGCATTTTGCTGCCTTGGTCCTGCAACTGCAACACTTTCTATGGTTTTGGNTGGCAT TGCATAACTTTCCTCGACTTTATGGANAGAGAGTTCANAAGTGTGAAACATACTGGCATA ACTCTCTGCCCAGGTCATATCATTGCAGTCAGATGAATATTTAAACCCATATTCATAATC TGTCCCAAATTATGGCTCTCACTTCACTTTTCCCTTCTCTTCTTGGCTTTTCGGTTTATT AAGNGCTTGGACTTTCAATGCCGGGGGGGGCCTTGNCTTTGTGGCCCACTGATCTTATTG AAATTCCCCTACAAATAGATGGGCTTCCTTCCTGAAATTCTAACCTAAAAAAAAAAAGCT GCTTAAAAATGATTGGTTCCTAGGCCAGACCTTTCCAAAGGTCCCTTATCTTTGTTGTGG GCGGCTTTATTGGCAACCGAAACGGACCTGGACCGGTTCCAGGGGTTTAAACCTTGTCCA ATTCCGGCCCCTTTGGGGTTTTCCAAATTGACGGACTTAAAAACTTTTTAAACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016055 |
| Insert Size | 960 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016055.4, NP_057139.1 |
| RefSeq Size | 1538 bp |
| RefSeq ORF | 639 bp |
| Locus ID | 51642 |
| UniProt ID | Q96GC5 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 6p. Several transcript variants, some protein-coding and some non-protein coding, have been found for this gene. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 2. The resulting isoform (1) has the same N- and C-termini but is shorter compared to isoform 2. |
Documents
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Resources
Other Versions
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| RC203039L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC203039L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RG203039 | MRPL48 (tGFP-tagged) - Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein |
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