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HSD17B12 (NM_016142) Human Untagged Clone
CAT＃: SC114479
HSD17B12 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 12 (HSD17B12)
CNY 3,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KAR; SDR12C1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114479 sequence for NM_016142 edited (data generated by NextGen Sequencing)
ATGGAGAGCGCTCTCCCCGCCGCCGGCTTCCTGTACTGGGTCGGCGCGGGCACCGTGGCC TACCTAGCCCTGCGTATTTCGTACTCGCTCTTCACGGCCCTCCGGGTCTGGGGAGTGGGG AATGAGGCGGGGGTCGGCCCGGGGCTCGGAGAATGGGCAGTTGTCACAGGTAGTACTGAT GGAATTGGAAAATCATATGCAGAAGAGTTAGCAAAGCATGGAATGAAGGTTGTCCTTATC AGCAGATCAAAGGATAAACTTGACCAGGTTTCCAGTGAAATAAAAGAAAAATTCAAAGTG GAGACAAGAACCATTGCTGTTGACTTTGCATCAGAAGATATTTATGATAAAATTAAAACA GGCTTGGCTGGTCTTGAAATCGGCATCTTAGTGAACAACGTGGGAATGTCGTATGAGTAT CCTGAATACTTTTTGGATGTTCCTGACTTGGACAATGTGATCAAGAAAATGATAAATATT AATATTCTTTCTGTTTGTAAGATGACACAATTGGTACTGCCTGGCATGGTGGAAAGATCC AAAGGGGCTATTCTGAACATTTCATCTGGCAGTGGCATGCTCCCTGTCCCACTCTTGACC ATCTATTCTGCAACCAAGACTTTTGTAGATTTCTTCTCTCAGTGCCTCCATGAGGAGTAT AGGAGCAAGGGCGTCTTTGTGCAGAGTGTCCTGCCATACTTCGTAGCTACAAAACTGGCT AAAATCCGGAAGCCAACTTTGGATAAGCCCTCTCCGGAGACGTTTGTGAAGTCTGCAATT AAAACAGTCGGCCTGCAATCCCGAACCAATGGATACCTGATCCATGCTCTTATGGGCTCG ATAATCTCAAACCTGCCTTCTTGGATTTATTTGAAAATAGTCATGAATATGAACAAGTCT ACACGGGCTCACTATCTGAAGAAAACCAAGAAGAACTAA Clone variation with respect to NM_016142.2 >OriGene 5' read for NM_016142 unedited
NGGTAACGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGGCACGAGGG GAATGGCCCCGCGGGCGGGGTTTAGGCCCAAATGGTGTCGGAGCAGCGCCTATTAGTGTC ATCCTCACCGTCACGGCCGGCGCCTCCTCCTGGATTCATTCACTCGCTCTTTTCATTCAC GAAGGTAGTGAGGCCTAGTGGAAAGCCATGGAGAGCGCTCTCCCCGCCGCCGGCTTCCTG TACTGGGTCGGCGCGGGCACCGTGGCCTACCTAGCCCTGCGTATTTCGTACTCGCTCTTC ACGGCCCTCCGGGTCTGGGGAGTGGGGAATGAGGCGGGGGTCGGCCCGGGGCTCGGAGAA TGGGCAGTTGTCACAGGTAGTACTGATGGAATTGGAAAATCATATGCAGAAGAGTTAGCA AAGCATGGAATGAAGGTTGTCCTTATCAGCAGATCAAAGGATAAACTTGACCAGGTTTCC AGTGAAATAAAAGAAAAATTCAAAGTGGAGACAAGAACCATTGCTGTTGACTTTGCATCA GAAGATATTTATGATAAAATTAANACAGGCTTGGCTGGTCTTGAAATCGGCATCTTAGTG AACAACGTGNGAATGTCGTATGAGTATCCTGAATACTTTTTGGATGTTCCTGACTTGGAC AATGTGATCAAGGAAATGATAAATATTAATATTCTTTCTGTTTGTAAGATGACACAATTG GTACTGCCTGGCATGGTGGAAAGATCCCAAGGGGCTATTCTGAACATTTCATCTGGCAGT GGCATGCGTCCTGTCCCACTCTTGACCATCTATTCTGCAACCAAAGACTTTTGTAAATTT CTTCTCTCAGTGCCTTCCATGAGGAGTATAGNACCAAGGGGCGTCTTTTGTGCCGAATGT CCCTGCCTAC >OriGene 3' read for NM_016142 unedited
GCCCATTACTATGNACCGCGGCACGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTCA TAAAGCTATATATTTATTCAACTTAAATGACAACCTTTATTCTGAGATTACATTCTTCCT AATTTTAGGGGCTAAAATATATTTTCTTTTACATAATGATGCTGATAGCAGATGGCTATT TGTTTTTGTTTGTTTATTTTTGTTTTATGTCCTTAGCTGGCAAAATTAAAAGCCAGAAGC TAAGGCCCCATTTTTTTTTCTTTTAATACAAATCTACTGGTGCTGAAAACTCAAAGCTTA GGAAACACAGCCTAGGTAAAGACCAATCTTCTTGCTGCATATTTCACAGTATTGAATTCT TTCTTGGCGAGTTCTCCATACAAGTTATGAAGCAGGATAAAAGTCACTCTTATATTAAGC CATTGTAAATACGGCCTTAATTTCACTCACCACACAACTGTATTTTTGTTGTACACCATG GTCGCACCATGTTTTATTAAACCTAACTCTATCACTAGCTACCTGCACAGTATCAACTTA CAAATTATACTGCCATTTCTATGAAGCCATCACTTTTATAGGACTACCCCGTGCCATATG TGTGACCAATATTGGCTTAATGCATAAACAAAGGCAGCTTGTGTGTCCAAGCAAGCCATT TTTCTGGGCCACGGTCTTAGTGCCACCCTATATGGCTGCCAGTCTCCATGTACACTCTGG CCCATTTATTTTTTATAACCATCCCATATAGCCCCGACTTCCCCCTACCTTTACACTGAA CACAACCTTCCTCTATAGAACGCTTACTCTAACGGCGCTCCATGGGCCACAAATACTTTC ATATTTACACTTATTTCCTTGCCTCTCACTCCCTTCGAAAACAACATTCCCTCATACCGC CCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016142 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016142.1, NP_057226.1 |
| RefSeq Size | 2393 bp |
| RefSeq ORF | 939 bp |
| Locus ID | 51144 |
| UniProt ID | Q53GQ0 |
| Domains | adh_short |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Androgen and estrogen metabolism, Biosynthesis of unsaturated fatty acids, Metabolic pathways |
| Gene Summary | This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of Hydroxysteroid (17β) dehydrogenase type 12 (HSD17B12) as a CD8+ T-cell-defined human tumor antigen of human carcinomas
,null,
Cancer immunology, immunotherapy : CII
,PubMed ID 21409596
[HSD17B12]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202385 | HSD17B12 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 12 (HSD17B12) |
CNY 2,400.00 |
|
| RC202385L3 | Lenti-ORF clone of HSD17B12 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 12 (HSD17B12) |
CNY 5,890.00 |
|
| RC202385L4 | Lenti-ORF clone of HSD17B12 (mGFP-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 12 (HSD17B12) |
CNY 5,890.00 |
|
| RG202385 | HSD17B12 (tGFP-tagged) - Human hydroxysteroid (17-beta) dehydrogenase 12 (HSD17B12) |
CNY 4,000.00 |
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