VPS28 (NM_016208) Human Untagged Clone

CAT#: SC114425

VPS28 (untagged)-Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1



  "NM_016208" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
VPS28 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC114425 sequence for NM_016208 edited (data generated by NextGen Sequencing)
ATGTTTCATGGGATCCCAGCCACGCCGGGCATAGGAGCCCCTGGGAACAAGCCGGAGCTG
TATGAGGAAGTGAAGTTGTACAAGAACGCCCGGGAGAGGGAGAAGTACGACAACATGGCA
GAGCTGTTTGCGGTGGTGAAGACAATGCAAGCCCTGGAGAAGGCCTACATCAAGGACTGT
GTCTCCCCCAGCGAGTACACTGCAGCCTGCTCCCGGCTCCTGGTCCAATACAAAGCTGCC
TTCAGGCAGGTCCAGGGCTCAGAAATCAGCTCTATTGACGAATTCTGCCGCAAGTTCCGC
CTGGACTGCCCGCTGGCCATGGAGCGGATCAAGGAGGACCGGCCCATCACCATCAAGGAC
GACAAGGGCAACCTCAACCGCTGCATCGCAGACGTGGTCTCGCTCTTCATCACGGTCATG
GACAAGCTGCGCCTGGAGATCCGCGCCATGGATGAGATCCAGCCCGACCTGCGAGAGCTG
ATGGAGACCATGCACCGCATGAGCCACCTCCCACCCGACTTTGAGGGCCGCCAGACGGTC
AGCCAGTGGCTGCAGACCCTGAGCGGCATGTCGGCGTCAGATGAGCTGGACGACTCACAG
GTGCGTCAGATGCTGTTCGACCTGGAGTCAGCCTACAACGCCTTCAACCGCTTCCTGCAT
GCCTGA

Clone variation with respect to NM_016208.2
>OriGene 5' read for NM_016208 unedited
NGGTTCACATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTG
CCGAATTCGGCACGAGGCGCCCCCGGCGCCATCTTCCCGACCGCGAGCCGTCCAGGTCTC
AGTGCTGTGCCCCCCCCAGAGCCTAGAGGATGTTTCATGGGATCCCAGCCACGCCGGGCA
TAGGAGCCCCTGGGAACAAGCCGGAGCTGTATGAGGAAGTGAAGTTGTACAAGAACGCCC
GGGAGAGGGAGAAGTACGACAACATGGCAGAGCTGTTTGCGGTGGTGAAGACAATGCAAG
CCCTGGAGAAGGCCTACATCAAGGACTGTGTCTCCCCCAGCGAGTACACTGCAGCCTGCT
CCCGGCTCCTGGTCCAATACAAAGCTGCCTTCAGGCAGGTCCAGGGCTCAGAAATCAGCT
CTATTGACGAATTCTGCCGCAAGTTCCGCCTGGACTGCCCGCTGGCCATGGAGCGGATCA
AGGAGGACCGGCCCATCACCATCAAGGACGACAAGGGCAACCTCAACCGCTGCATCGCAG
ACGTGGTCTCGCTCTTCATCACGGTCATGGACAAGCTGCGCCTGGAGATCCGCGCCATGG
ATGAGATCCAGCCCGACCTGCGAGAGCTGATGGAGACCATGCACCGCATGAGCCACCTCC
CACCCGACTTTGAGGGCCGCCAGACGGTCAGCCAGTGGCTGCAGACCCTGAGCGGCATGT
CGGCGTCAGATGAGCTGGACGACTCACAGGTGCGTCAGATGCTGTTCGACCTGGAGTCAG
CCTACAACGCCTTTCACCGCTTNCTGCATGCCTGAGCCCGGGCACTAGCCCTTGCACAGA
AGGCAGAGTCTGAGGCGATGGCTCCTGGTCCCCTGTCCGCCACACAGCCGTGGTCATCCC
N
>OriGene 3' read for NM_016208 unedited
GCCCCCAGCAGGAGGTCGGNANAGCTCTTTATTGTGGGGGAGGGGGGCCCGGCCCCCAAA
GTTCTGACACCAAAGACAGACACCAGACAGGCAGCTGCAGACAGTGAGTTGTGTGGATGA
CCACGGCCTGTGTGGCGGACAGGGGACCAGGAGCCATCGCCTCAGACTCTGCCCTTCTGT
GCAAGGGCTAGTGCCCCGGGCTCAGGCATGCAGGAAGCGGTTGAAGGCGTTGTAGGCTGA
CTCCAGGTCGAACAGCATCTGACGCACCTGTGAGTCGTCCAGCTCATCTGACGCCGACAT
GCCGCTCAGGGTCTGCAGCCACTGGCTGACCGTCTGGCGGCCCTCAAAGTCGGGTGGGAG
GTGGCTCATGCGGTGCATGGTCTCCATCAGCTCTCGCAGGTCGGGCTGGATCTCATCCAT
GGCGCGGATCTCCAGGCGCAGCTTGTCCATGACCGTGATGAAGAGCGAGACCACGTCTGC
GATGCAGCGGTTGAGGTTGCCCTTGTCGTCCTTGATGGTGATGGGCCGGTCCTCCTTGAT
CCGCTCCATGGCCAGCGGGCAGTCCAGGCGGAACTTGCGGCAGAATTCGTCAATAGAGCT
GATTTCTGAGCCCTGGACCTGCCTGANAGCAGCTNTGTATTGGACCAGGAGCCGGGAGCA
GGCTGCAGTGTACTCGCTGGGGGAGACACAGTCCTTGATGTAGGCCTTCTCCAGGGCTTG
CATTGTCTTCACCACCGCANACAGCTCTGCCATNGTGTCGTACTTNCTCCTCTNCCCGGG
CGTNCTGTACAACTTCACTTNCTCATACAGCTCCGGCTTGNTCCCAGGGGGCTCTATGNC
CCGCGTGGCTGGGATCCCATGAAACATNCCTCTAGCTCTGGNGGGGGCACAGCACTGAAA
CCTGNNACGCTCGCGGTCGGNAAGATGCGCCGGGGGCGCCTCGTGCCGAAATCGCACGAG
CCTNTGCCNCGATCNGGG
Restriction Sites NotI-NotI     
ACCN NM_016208
Insert Size 1150 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016208.2, NP_057292.1
RefSeq Size 1065 bp
RefSeq ORF 666 bp
Locus ID 51160
UniProt ID Q9UK41
Domains VPS28
Protein Pathways Endocytosis
Gene Summary This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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