SELT (SELENOT) (NM_016275) Human Untagged Clone

CAT#: SC114373

SELT (untagged)-Human selenoprotein T (SELT) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)



  "NM_016275" in other vectors (3)

CNY 3,432.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Synonyms SELT
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_016275, the custom clone sequence may differ by one or more nucleotides


ATGAGGCTTCTGCTGCTTCTCCTAGTGGCGGCGTCTGCGATGGTCCGGAGCGAGGCCTCGGCCAATCTGG
GCGGCGTGCCCAGCAAGAGATTAAAGATGCAGTACGCCACGGGGCCGCTGCTCAAGTTCCAGATTTGTGT
TTCCTGAGGTTATAGGCGGGTGTTTGAGGAGTACATGCGGGTTATTAGCCAGCGGTACCCAGACATCCGC
ATTGAAGGAGAGAATTACCTCCCTCAACCAATATATAGACACATAGCATCTTTCCTGTCAGTCTTCAAAC
TAGTATTAATAGGCTTAATAATTGTTGGCAAGGATCCTTTTGCTTTCTTTGGCATGCAAGCTCCTAGCAT
CTGGCAGTGGGGCCAAGAAAATAAGGTTTATGCATGTATGATGGTTTTCTTCTTGAGCAACATGATTGAG
AACCAGTGTATGTCAACAGGTGCATTTGAGATAACTTTAAATGATGTACCTGTGTGGTCTAAGCTGGAAT
CTGGTCACCTTCCATCCATGCAACAACTTGTTCAAATTCTTGACAATGAAATGAAGCTCAATGTGCATAT
GGATTCAATCCCACACCATCGATCATAG


>OriGene 5' read for NM_016275 unedited
GGTTCACCATTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCCGAA
GTGGCTGGCTCATTTAAGATGAGGCTTCTGCTGCTTCTCCTAGTGGCGGCGTCTGCGATG
GTCCGGAGCGAGGCCTCGGCCAATCTGGGCGGCGTGCCCAGCAAGAGATTAAAGATGCAG
TACGCCACGGGGCCGCTGCTCAAGTTCCAGATTTGTGTTTCCTGAGGTTATAGGCGGGTG
TTTGAGGAGTACATGCGGGTTATTAGCCAGCGGTACCCAGACATCCGCATTGAAGGAGAG
AATTACCTCCCTCAACCAATATATAGACACATAGCATCTTTCCTGTCAGTCTTCAAACTA
GTATTAATAGGCTTAATAATTGTTGGCAAGGATCCTTTTGCTTTCTTTGGCATGCAAGCT
CCTAGCATCTGGCAGTGGGGCCAAGAAAATAAGGTTTATGCATGTATGATGGTTTTCTTC
TTGAGCAACATGATTGAGAACCAGTGTATGTCAACAGGTGCATTTGAGATAACTTTAAAT
GATGTACCTGTGTGGTCTAAGCTGGAATCTGGTCACCTTCCATCCATGCAACAACTTGTT
CAAATTCTTGACAATGAAATGAAGCTCAATGTGCATATGGATTCAATCCCCCACCATTCG
ATCATAGCACCACCCTATCAGCACTGAAAACTCTTTTGCATTAACGGATCATTGCAAGAG
CAGCGTGACTGACATTATGAAGGCCTGTACTGAACACAGCAAGCTGTTAGTACAGACCAG
ATGCTTTCTTGCAGGCTCGTTGTCCCTCTTGGAAAACTTCAAGCAAGAAAGTGTTTCAGT
GCTGGCATATTTTGAAATCTGCACATTCTGGAGGGCAATATACTGTTTAGCTTTCCCCAC
CTCCACAAAT
Restriction Sites NotI-NotI     
ACCN NM_016275
Insert Size 4600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016275.3, NP_057359.2
RefSeq Size 3498 bp
Locus ID 51714
UniProt ID P62341
Gene Summary This gene encodes a selenoprotein, containing a selenocysteine (Sec) residue at the active site. Sec is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is localized in the endoplasmic reticulum. It belongs to the SelWTH family that possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif found in several redox active proteins. Studies in mice indicate a crucial role for this gene in the protection of dopaminergic neurons against oxidative stress in Parkinson's disease, and in the control of glucose homeostasis in pancreatic beta-cells. Pseudogenes of this locus have been identified on chromosomes 9 and 5. [provided by RefSeq, Sep 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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