Methionine Sulfoxide Reductase B (MSRB1) (NM_016332) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Synonyms	HSPC270; SELENOR; SELENOX; SELR; SELX; SepR; SEPX1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC114321 sequence for NM_016332 edited (data generated by NextGen Sequencing) ATGTCGTTCTGCAGCTTCTTCGGGGGCGAGGTTTTCCAGAATCACTTTGAACCTGGCGTT TACGTGTGTGCCAAGTGTGGCTATGAGCTGTTCTCCAGCCGCTCGAAGTATGCACACTCG TCTCCATGGCCGGCGTTCACCGAGACCATTCACGCCGACAGCGTGGCCAAGCGTCCGGAG CACAATAGATCTGAAGCCTTGAAGGTGTCCTGTGGCAAGTGTGGCAATGGGTTGGGCCAC GAGTTCCTGAACGACGGCCCCAAGCCGGGGCAGTCCCGATTCTGAATATTCAGCAGCTCG CTGAAGTTTGTCCCTAAAGGCAAAGAAACTTCTGCCTCCCAGGGTCACTAG Clone variation with respect to NM_016332.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_016332 unedited GGTGCACTATTTTGTAATACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGG GCAAGCGCGCTGCGGTTCCGGTGGCGCCATGTCGTTCTGCAGCTTCTTCGGGGGCGAGGT TTTCCAGAATCACTTTGAACCTGGCGTTTACGTGTGTGCCAAGTGTGGCTATGAGCTGTT CTCCAGCCGCTCGAAGTATGCACACTCGTCTCCATGGCCGGCGTTCACCGAGACCATTCA CGCCGACAGCGTGGCCAAGCGTCCGGAGCACAATAGATCTGAAGCCTTGAAGGTGTCCTG TGGCAAGTGTGGCAATGGGTTGGGCCACGAGTTCCTGAACGACGGCCCCAAGCCGGGGCA GTCCCGATTCTGAATATTCAGCAGCTCGCTGAAGTTTGTCCCTAAAGGCAAAGAAACTTC TGCCTCCCAGGGTCACTAGGCGGGCAGCCCACACCCACCCCAGACGGTCACCACACTGAG GCCACACGTTGGCCATTCCACCTTGGAGTTGGAACCCTGCGCGTCGAGACAGGAAGGCAG GGCGCAGTGGTTGAAACATCAGGACACTCCCAAGGCCCCGGCTCTGAACAAGACCTTTTC GTTTCTTGGAAAAGAGACTCATTTGCTGATGGTTCATGCCTTCTGCTGGGACAGGCCTGT GCTGTGCAGCCACACTGTCGGCTGACTTTAGCCCCTGCTCACTCTAGGTGCCTCCAGGAG GTGAGCCCTGNGTGCAGCCTGGTCTCTGAATGACGTTACACCCTCACCTTCTTTTCCTGG CCCTGTCTCTTGAACTCTCCCCTGTGAGGGCCCAATTTCACAGACAGACTCTCGTCCTCA CCGAAGCCTTAGCCCCACATTTCCCAGGCTGTTTATGAGACAGAATGGAACGGAGGCCGG CCCTGTCAACCGCA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_016332 unedited TTGTCTAGGCTATTCCCCTGCTTACTCCATAAGTAATTACTTAATAGGGGGGTGATTTCA GCATCACCCACCCTCCTATATGGCCAGGCCTCCGAGCATGTACAGTAAGGCACAGTCACC ACACCTTGGGAAGGGCCTCCCTGCATGTGTGCACTGCATGCCTCATCCCTGTGCTGGTTA GAGTTATGGAGCAACAGCCGCTTTCCTAAGCAGAAGTGATGCAAGCTACTTCCGCACAGA TTGTGAAACAGGGTTTATGTTTCTCCCCTCGTGGACGAGATGCCAAGGGTGTGCCCCATC ATCCTGCCTTCCCAGCAGGTCACAGACCATCCCCACTCTGGCTGGCCTGGAAGGGTTTGA CCAGAGCGGATCATGCAGTGACCAGAGCCAGGGCGGCTGGCAGGGGCGGCCTCCGTTTCC ATTCTGTCTCCTAAGCAGCCTGAGAGATGTGGGCCTAAGCTTCGGTGATGACGAGAGTCT GTCTTGGAATTGGGCCTCACAGGGGAGAGTCCAGAGACAGGGCCAGGAAAAGAATGTGAG GGTGTAACGTCATTCATAGACCAGCTGCACCCATAGCTCACCTCCTGGATGCACCTATAG TGATCATGGGGCTAAGTCAGCCGACAGTGTGGCTGCACAGCCCATGCCTGTCCCATCAGA TAGCATGAACCATCAGCATATGAGTCTCTCTTCCAAGAACGAAAAGGTCTGTTCATATCC GAGCCTTGAGAGTGTCCTAGATGTTCACCACTGCGCCCTGCCTTNCTGTCTCGACGCCCA TGGTTCAACTNCATGTGGAATGGCCAACGTGTGGNCCTCATGTGTGGCCGTCTGGGGTGG TGTGGGCTGCCCGCCTATGACCCTGGAAGCANAAGTTTCTTTGCCTTTAGGACAAATTCA CCGAGCTGCTGAAATCCAAATCTGGACTGCCCCCGCTTGAGCCGTCGTTCAGGACTCCTG GCCACCCATGC
Restriction Sites	NotI-NotI
ACCN	NM_016332
Insert Size	1360 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016332.2, NP_057416.1
RefSeq Size	1386 bp
Locus ID	51734
UniProt ID	Q9NZV6
Domains	SelR
Gene Summary	The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 19. [provided by RefSeq, Aug 2017]

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