Leucyl tRNA synthetase (LARS) (NM_016460) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hr025Cl; HSPC192; ILFS1; LARS; LEURS; LEUS; LFIS; LRS; PIG44; RNTLS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_016460, the custom clone sequence may differ by one or more nucleotides ATGGCGGAAAGAAAAGGAACAGCCAAAGTGGACTTTTTGAAGAAGATTGAGAAAGAAATCCAACAGAAAT GGGATACTGAGAGAGTGTTTGAGGTCAATGCATCTAATTTAGAGAAACAGACCAGCAAGGGCAAGTATTT TGTAACCTTCCCATATCCATATATGAATGGACGCCTTCATTTGGGACACACGTTTTCTTTATCCAAATGT GAGTTTGCTGTAGGGTACCAGCGATTGAAAGGAAGATGTTGTCTGTTTCCCTTTGGCCTGCACTGTACTG GAATGCCTATTAAGGCATGTGCTGATAAGTTGAAAAGAGAAATAGAGCTGTATGGTTGCCCCCCTGATTT TCCAGATGAAGAAGAGGAAGAGGAAGAAACCAGTGTTAAAACAGAAGATATAATAATTAAGGATAAAGCT AAAGGAAAAAAGAGTAAAGCTGCTGTAAAGCTGGATCTTCTAAATACCAGTGGGGCATTATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_016460 unedited NCGCGATAAATATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTTA GTTTCGTGGGAGGCCTGGCATCCCCGAGAGGGAGGGGAAAGGTAACCACTCCTTTGTGGA GGTCGCCAGGGTCATTGTCGTGGATTTGCACAGTCGGCTGGGCGGTGCAATGGCGGAAAG AAAAGGAACAGCCAAAGTGGACTTTTTGAAGAAGATTGAGAAAGAAATCCAACAGAAATG GGATACTNGAGAGAGTGTTTGAGGTCAATGCATCTAATTTAGAGAAACAGACCAGCAAGG GCAAGTATTTTGTAACCTTCCCATATCCATATATGAATGGACGCCTTCATTTGGGACACA CGTTTTCTTTATCCAAATGTGAGTTTGCTGTAGGGTACCAGCGATTGAAAGGAAAATGTT GTCTGTTTCCCTTTGGCCTGCACTGTACTGGAATGCCTATTAAGGCATGTGCTGATAAGT TGAAAAGAGAAATAGAGCTGTAATGGTTGCCCCCCTGATTTTCCAGATGAAGAAGAGGAA GAGGAAGAAACCAGTGTTAAAACAGAAGATATAATAATTAAGGATAAAGCTAAAGGAAAA AAGAGTAAAGCTGCTGCTAAAGCTGGATCTTCTAATACCAGTGGGGCATTATGAAATCCT TGGCCCTGTCTGATGAAAGAGATAGTAAATTTTCTGGAGCAGACATTGGGCTTNGATATT TCCNGCACTGGCTATNCNGATTAAAAGAAAGGGGNTNATAAAGAAAATGGGGGGGNNNTT CTTATACCCCCGGGAGATATCCTACAATGAATCCTTGGGCAAAGGGCATTTTTTACATTT AAGAGAAGAACAAATTAATTGGGGGAAAGGGGTTTCAATTACTCTCCGAAGATGACGCCC TGCCTGGATATGATAATGGAGAAGGAGGGGGGGGGGCCCACAAGGATTCACTTCA
Restriction Sites	NotI-NotI
ACCN	NM_016460
Insert Size	5000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016460.1, NP_057544.1
RefSeq Size	722 bp
RefSeq ORF	483 bp
Locus ID	51520
UniProt ID	Q9P2J5
Protein Families	Druggable Genome
Protein Pathways	Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
Gene Summary	This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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