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MARCHF2 (NM_016496) Human Untagged Clone
CAT＃: SC114251
41335 (untagged)-Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSPC240; MARCH-II; MARCH2; RNF172 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114251 sequence for NM_016496 edited (data generated by NextGen Sequencing)
ATGACGACGGGTGACTGCTGCCACCTCCCCGGCTCCCTGTGTGACTGCTCCGGCAGCCCT GCCTTCTCCAAGGTCGTGGAGGCTACGGGCCTCGGACCGCCCCAGTATGTGGCACAGGTG ACTTCAAGGGATGGCCGGCTCCTCTCCACCGTCATCCGTGCCTTGGACACACCGAGTGAT GGTCCTTTCTGCCGGATCTGCCATGAGGGAGCGAACGGGGAGTGCTTGCTGTCCCCGTGT GGCTGCACCGGCACGCTGGGTGCCGTGCATAAGAGCTGTCTGGAGAAGTGGCTTTCCTCA TCTAACACCAGCTACTGCGAGCTGTGCCACACGGAGTTTGCAGTGGAGAAACGGCCTCGA CCCCTCACAGAGTGGCTGAAGGACCCGGGGCCGCGGACGGAGAAGCGGACACTGTGCTGC GACATGGTGTGTTTCCTGTTCATCACACCGCTGGCCGCCATCTCAGGCTGGTTGTGCCTG CGCGGGGCCCAGGACCACCTCCGGCTCCACAGCCAGCTGGAGGCCGTGGGTCTCATTGCC CTCACCATCGCCCTCTTCACCATCTATGTCCTCTGGACGCTGGTCTCCTTCCGCTACCAC TGCCAGCTGTACTCCGAGTGGAGAAAGACCAACCAGAAAGTTCGCCTGAAGATCCGGGAG GCGGACAGCCCCGAGGGCCCCCAGCATTCTCCACTGGCAGCTGGACTCCTGAAGAAGGTG GCAGAGGAGACACCAGTATGA Clone variation with respect to NM_016496.4 >OriGene 5' read for NM_016496 unedited
AGAGTTAGAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCGGCCG GAGCGGAGCTAGTGGCGCCGACGGGCCGGGCCGGGCCGGGACCGGGGCCGAGGCGAACCG AGGGGCCTGTGCGGCCGCCCGGCCCGGCCGCGGATCAGGCTCCTGGAACCATGGGCCTCA GGCCCTGAGGATACGGGGCTCCCGGTGGCCATGACGACGGGTGACTGCTGCCACCTCCCC GGCTCCCTGTGTGACTGCTCCGGCAGCCCTGCCTTCTCCAAGGTCGTGGAGGCTACGGGC CTCGGACCGCCCCAGTATGTGGCACAGGTGACTTCAAGGGATGGCCGGCTCCTCTCCACC GTCATCCGTGCCTTGGACACACCGAGTGATGGTCCTTTCTGCCGGATCTGCCATGAGGGA GCGAACGGGGAGTGCTTGCTGTCCCCGTGTGGCTGCACCGGCACGCTGGGTGCCGTGCAT AAGAGCTGTCTGGAGAAGTGGCTTTCCTCATCTAACACCAGCTACTGCGAGCTGTGCCAC ACGGAGTTTGCAGTGGAGAAACGGCCTCGACCCCTCACAGAGTGGCTGAAGGACCCGGGG CCGCGGACGGAGAAGCGGACACTGTGCTGCGACATGGTGTGTTTCCTGTTCATCACACCG CTGGCCGCCATCTCAGGCTGGTTGTGCCTGCGCGGGGCCCAGGACCACCTCCGGCTCCAC AGCCAGCTGGAGGCCGTGGGTCTCATTGCCCTCACCATCGCCCTCTTCACCATCTATGTC CTCTGGACGCTGGTCTCCTTCCGCTACCACTGCCAGCTGTACTCCGAGTGGAGAAAGACC AACCAGAAAGTTCGCCTGAAGATCCGGNAGGCGGACAGCCCCGAGGGCCCCCAGCATTCT NCACTGGCAGCTGGACTCCTGAAGAAA >OriGene 3' read for NM_016496 unedited
CCGCGGGCCGCAATCTAGCATCGAGTTTTTTTTTTTTTTTTTTTTGCCCAGCCAGGGCTT CTTTTATTCATTCAGGGGTGGGTTTTTGAAACGCAGTGCAACTTTTATATAAAGCCGAGG GTGCCAGGAAAGTGGGCCTGCCGGGTGCAGAGAAGCGCAAGAAGCTTGCTGGAATGTTCC AGCTTTAAGGGCGCCCTTGATACACCGGCCACAGCTGCTGGCCCAGAAGGTGCCATGTGG ACCTTAGAATGAGATCAGGGTGCCTGCTACGTCCAGAGCTCAGGACCATGTCACACACTG TCCCTCCCATATGCCCAACAAAACCCCATCCTACCCCCCCCCCCCCCCCCCCCCCCTCCC CCCTACCCCCCCCCCTTCCCCTTTCTCCGTCTCCCCCCCCCCCCTCCCCCCTCTTCGTCC CCTCCTTCTCCTCTCCCCCCCCCCTCCCCCCTTCCCCCCCCCTCCCCCCCCCCTCCTCTC CTTTCCCCCCCTCCCCTCTCCTTCCCCTTACCGCTTCCCCTCCCTCTCCTCCCCCCCTCA CTTCCCCCCTTTCCCCCCCCCCTCCTCTTACCCGCCCGCCTTCCCCCCTTTTCCCATCCC CCCCCCCCTTACCTCTCCCCCGCTCCATTCCCCCTCCTCCCCTCCCCCCCCCCCATCCCC CTTCCCCCTTCTTCCCTCCCACTCTCCCCCTCCCCTTTCCCCATTCCCCATTCCTTTCTT TCCCCCTTTTCCTCTTCCCCCCCTTCCTCCTCCCCCCGCCGCCCCTGTCCCCGCCGTTTC TTCCCCCTCCTTTCCTCCCCATCCTCTTCGTCCGCCCCCTCCCCCTCCCCCCCCCCCCTT CCCCCCCCCCTTCTCTCTTCTCCCCCGCCGTTATTTGCACACCATCACCCCCTTCCCCCC CCCCCCCCTCTCCCTTTCCTGTCCCGTCCACCCT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_016496 |
| Insert Size | 1250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016496.3, NP_057580.2 |
| RefSeq Size | 1745 bp |
| RefSeq ORF | 741 bp |
| Locus ID | 51257 |
| UniProt ID | Q9P0N8 |
| Domains | RING |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Variants 1, 2, and 4-7 all encode the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound ß2-adrenergic receptors
,Sang-oh Han, Kunhong Xiao, Jihee Kim, Jiao-Hui Wu, James W. Wisler, Nobuhiro Nakamura, Neil J. Freedman, and Sudha K. Shenoy,
J. Cell Biol., Nov 2012; 199: 817 - 830.
[MARCHF2]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207517 | MARCH2 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1 |
CNY 2,400.00 |
|
| RC207517L1 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207517L2 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207517L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207517L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG207517 | 41335 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1 |
CNY 4,000.00 |
|
| SC323978 | 41335 (untagged)-Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1 |
CNY 2,400.00 |
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