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NT5C3 (NT5C3A) (NM_001002010) Human Untagged Clone
CAT＃: SC114247
NT5C3 (untagged)-Human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 1
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | cN-III; hUMP1; NT5C3; P5'N-1; P5N-1; p36; PN-I; POMP; PSN1; UMPH; UMPH1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114247 sequence for NM_001002010 edited (data generated by NextGen Sequencing)
ATGAGGGCCCCGTCCATGGACCGCGCGGCCGTGGCGAGGGTGGGCGCGGTAGCGAGCGCC AGCGTGTGCGCCCTGGTGGCGGGGGTGGTGCTGGCTCAGTACATATTCACCTTGAAGAGG AAGACGGGGCGGAAGACCAAGATCATCGAGATGATGCCAGAATTCCAGAAAAGTTCAGTT CGAATCAAGAACCCTACAAGAGTAGAAGAAATTATCTGTGGTCTTATCAAAGGAGGAGCT GCCAAACTTCAGATAATAACGGACTTTGATATGACACTCAGTAGATTTTCATATAAAGGG AAAAGATGCCCAACATGTCATAATATCATTGACAACTGTAAGCTGGTTACAGATGAATGT AGAAAAAAGTTATTGCAACTAAAGGAAAAATATTACGCTATTGAAGTTGATCCTGTTCTT ACTGTAGAAGAGAAGTACCCTTATATGGTGGAATGGTATACTAAATCACATGGTTTGCTT GTTCAGCAAGCTTTACCAAAAGCTAAACTTAAAGAAATTGTGGCAGAATCTGACGTTATG CTCAAAGAAGGATATGAGAATTTCTTTGATAAGCTCCAACAACATAGCATCCCCGTGTTC ATATTTTCGGCTGGAATCGGCGATGTACTAGAGGAAGTTATTCGTCAAGCTGGTGTTTAT CATCCCAATGTCAAAGTTGTGTCCAATTTTATGGATTTTGATGAAACTGGGGTGCTCAAA GGATTTAAAGGAGAACTAATTCATGTATTTAACAAACATGATGGTGCCTTGAGGAATACA GAATATTTCAATCAACTAAAAGACAATAGTAACATAATTCTTCTGGGAGACTCCCAAGGA GACTTAAGAATGGCAGATGGAGTGGCCAATGTTGAGCACATTCTGAAAATTGGATATCTA AATGATAGAGTGGATGAGCTTTTAGAAAAGTACATGGACTCTTATGATATTGTTTTAGTA CAAGATGAATCATTAGAAGTAGCCAACTCTATTTTACAGAAGATTCTATAA Clone variation with respect to NM_001002010.1 >OriGene 5' read for NM_001002010 unedited
TATAGGGCGGCCGCGAATTCGCACGAGGCTGGGATCCCGCGCTTCCGAGACTCGCAGTCT ACGCGAGCTGCCTGTTTTTTTCCTGCTTGGACGCGCATGAGGGCCCCGTCCATGGACCGC GCGGCCGTGGCGAGGGTGGGCGCGGTAGCGAGCGCCAGCGTGTGCGCCCTGGTGGCGGGG GTGGTGCTGGCTCAGTACATATTCACCTTGAAGAGGAAGACGGGGCGGAAGACCAAGATC ATCGAGATGATGCCAGAATTCCAGAAAAGTTCAGTTCGAATCAAGAACCCTACAAGAGTA GAAGAAATTATCTGTGGTCTTATCAAAGGAGGAGCTGCCAAACTTCAGATAATAACGGAC TTTGATATGACACTCAGTAGATTTTCATATAAAGGGAAAAGATGCCCAACATGTCATAAT ATCATTGACAACTGTAAGCTGGTTACAGATGAATGTAGAAAAAAGTTATTGCAACTAAAG GAAAAATATTACGCTATTGAAGTTNGATCCTGTTCTTACTGTAGAAGAGAAGTACCCTTA TATGGTGGAATGGTATACTAAATCACATGGTTTTGCTTGTTCAGCAAGCTTTACCAAAAG CTAAACTTAAAGAAATTGTGGCAGATNNCTGACGTTATGCTCANAGAAGGATATGAAGAA ATTTCTTTGATAAGCTCCAACACATAGCATCCCCCGTGTTCATATTTTTCGGCTGGATCG GGCGATGACTTNAAAGGAAGTTATTCGTCAGCTGGGTGTTTATATCCATGTCAAAAATTG TGTCATTTATAAGGGATTTGATAAACCGGGGGTGCTCAAAGATTTAAAGGAGACTAATTC ATGTATTTACAACCTTGAGGGGCCTTTGAGGATACAGAATATTTAATCACTAAA >OriGene 3' read for NM_001002010 unedited
NNTTTTTACTCTGGACNCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTA AAAGGTTTTGCATTTCATATTTATTATCAGTGCTTCAATATAGAATGTTTTGTAATGATT AGCAACATTGTAAACACCACCAGGTTTTTCCAACACTAACTGCTAGTCTTTTCCAGTAAA TATTTTTTCCCCAGACAAAATAACCAAACCTAACACAATTTCAAGAGATGGTGATACCAT CAGCATAATAACCCAAATTACAAAAATGGCAATACTTAAAATCATGCATATTATTTCACC ATTTCATAAAACTTATTTTAAAATTTCTACAAACTTTCCCAGTGTAAAAGACTCCTCAAA AGCTGCATGATAACATTAAACTACAAAATTCACTTTGGAAAAGTAGGGAGTTATTTTTCT AATTTTAGCTTTTTTTTTTTTAAATGGGTTAAAAGATACGGTGAGGAGTGTGTTGAGAGA GGTGGAGAAAAGGAGCTTCCAGTCAATGCATTCACCATATCTGAAAATACTTCAGTTATA CAAAGGGAACACTTCGAGAGTAAGGATATATTATAAATAAGTCTCTCAGCAAGATGAACG GATGAACAGTTCAATTGCACCCACAGGAGAGAGGTCTTCTTGGAGAATGCTTGTTTATAG AATCTTCTGTAAAATAGAGTTGGCTACTTCTAATGATTCATCTTGTACTAAAACAATATC ATAAGAGTCCATGTACTTTTCTAAAAGCTCATCCACTCTATCATTTAGATATCCAATTTT CAGAATGTGCTCAACATTGGCCACTNCATCTGCCATTCTTAAAGTCTCTTGGGAGTCTNC CAGAGAATATGNTACTATTGGCTTTTAGNNTGATGAAAAATCTGTATCCTCAGGCACCAT CATGNNTGTAATACAGAAATAGNTCTCTTTAAATCTTGAGCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001002010 |
| Insert Size | 1840 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001002010.1, NP_001002010.1 |
| RefSeq Size | 1688 bp |
| RefSeq ORF | 1011 bp |
| Locus ID | 51251 |
| UniProt ID | Q9H0P0 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). CCDS Note: The coding region has been updated to start at a downstream in-frame start codon that is more supported by conservation data. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222818 | NT5C3 (Myc-DDK-tagged)-Human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 1 |
CNY 5,488.00 |
|
| RC222818L3 | Lenti ORF clone of Human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222818L4 | Lenti ORF clone of Human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG222818 | NT5C3 (tGFP-tagged) - Human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 1 |
CNY 4,370.00 |
