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DACT1 (NM_016651) Human Untagged Clone
CAT＃: SC114181
DACT1 (untagged)-Human dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1
CNY 16,250.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAPPER; DAPPER1; DPR1; FRODO; HDPR1; TBS2; THYEX3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NCBI ORF sequence for NM_016651, the custom clone sequence may differ by one or more nucleotides
ATGAAGCCGAGTCCGGCCGGGACGGCGAAGGAGCTGGAGCCTCCGGCGCCGGCCCGAGGCGAGCAGCGCA CGGCGGAGCCCGAGGGGCGCTGGCGGGAGAAGGGCGAGGCAGACACCGAGCGGCAGCGCACCCGGGAGCG GCAGGAGGCCACGCTGGCCGGGCTGGCGGAGCTGGAGTACCTGCGCCAGCGCCAAGAGCTGCTGGTCAGG GGCGCCCTGCGCGGCGCCGGGGGTGCGGGAGCCGCTGCGCCCCGCGCTGGGGAGCTACTGGGGGAGGCGG CGCAGCGCAGTCGCCTGGAGGAGAAGTTCTTGGAGGAGAACATCTTGCTGCTAAGAAAGCAATTGAACTG TTTGAGGCGAAGAGATGCTGGTTTGTTGAATCAGTTGCAAGAGCTTGACAAGCAGATAAGTGACCTGAGA CTGGATGTAGAAAAGACATCTGAAGAGCACCTGGAGACAGACAGTCGGCCTAGCTCAGGGTTTTATGAGC TGAGTGATGGGGCTTCAGGATCCCTTTCCAATTCCTCTAACTCGGTGTTCAGTGAGTGTTTATCCAGTTG TCATTCCAGCACCTGCTTTTGCAGCCCCTTGGAGGCGACCTTGAGTCTCTCAGATGGTTGCCCCAAATCT GCAGATCTCATAGGATTGTTGGAATATAAAGAAGGCCACTGTGAAGACCAGGCCTCAGGGGCAGTTTGCC GTTCCCTCTCCACACCACAATTTAATTCCCTTGATGTCATTGCAGATGTGAATCCCAAGTACCAGTGTGA TCTGGTGTCTAAAAACGGGAATGATGTATATCGCTATCCCAGTCCACTTCATGCTGTGGCTGTGCAGAGC CCAATGTTTCTCCTTTGTCTGACGGGCAACCCTCTGAGGGAAGAGGACAGGCTTGGAAACCATGCCAGTG ACATTTGCGGTGGATCTGAGCTAGATGCCGTCAAAACAGACAGTTCCTTACCGTCCCCAAGCAGTCTGTG GTCTGCTTCCCATCCTTCATCCAGCAAGAAAATGGATGGCTACATTCTGAGCCTGGTCCAGAAAAAAACA CACCCTGTAAGGACCAACAAACCAAGAACCAGCGTGAACGCTGACCCCACGAAAGGGCTTCTGAGGAACG GGAGCGTTTGTGTCAGAGCCCCGGGCGGTGTCTCACAGGGCAACAGTGTGAACCTTAAGAATTCGAAACA GGCGTGTCTGCCCTCTGGCGGGATACCTTCTCTGAACAATGGGACATTCTCCCCACCGAAGCAGTGGTCG AAAGAATCAAAGGCCGAACAAGCCGAAAGCAAGAGGGTGCCCCTGCCAGAGGGCTGCCCCTCAGGCGCTG CCTCCGACCTTCAGAGTAAGCACCTGCCAAAAACGGCCAAGCCAGCCTCGCAAGAACATGCTCGGTGTTC CGCCATTGGGACAGGGGAGTCCCCTAAGGAAAGCGCTCAGCTCTCAGGGGCCTCTCCAAAAGAGAGTCCT AGCAGAGGCCCTGCCCCGCCGCAGGAGAACAAAGTTGTACAGCCCCTGAAAAAGATGTCACAGAAAAACA GCCTGCAGGGCGTCCCCCCGGCCACTCCTCCCCTGCTGTCTACAGCTTTCCCCGTGGAAGAGAGGCCTGC CTTGGATTTCAAGAGCGAGGGCTCTTCCCAAAGCCTGGAGGAAGCGCACCTGGTCAAGGCCCAGTTTATC CCGGGGCAGCAGCCCAGTGTCAGGCTCCACCGGGGCCACAGGAACATGGGCGTCGTGAAGAACTCCAGCC TGAAGCACCGCGGCCCAGCCCTCCAGGGGCTGGAGAACGGCTTGCCCACCGTCAGGGAGAAAACGCGGGC CGGGAGCAAGAAGTGTCGCTTCCCAGATGACTTGGATACAAATAAGAAACTCAAGAAAGCCTCCTCCAAG GGGAGGAAGAGTGGGGGCGGGCCCGAGGCTGGTGTTCCCGGCAGGCCCGCGGGCGGGGGCCACAGGGCGG GGAGCAGGGCGCATGGCCACGGACGGGAGGCGGTGGTGGCCAAACCTAAGCACAAGCGAACTGACTACCG GCGGTGGAAGTCCTCGGCCGAGATTTCCTACGAAGAGGCCCTGAGGAGGGCCCGGCGCGGTCGCCGGGAG AATGTGGGGCTGTACCCCGCGCCTGTGCCTCTGCCCTACGCCAGCCCCTACGCCTACGTGGCTAGCGACT CCGAGTACTCGGCCGAGTGCGAGTCCCTGTTCCACTCCACCGTGGTGGACACCAGTGAGGACGAGCAGAG CAATTACACCACCAACTGCTTCGGGGACAGCGAGTCGAGTGTGAGCGAGGGCGAGTTCGTGGGGGAGAGC ACAACCACCAGCGACTCTGAAGAAAGCGGGGGCTTAATTTGGTCCCAGTTTGTCCAGACTCTGCCCATTC AAACGGTAACGGCCCCAGACCTTCACAACCACCCCGCAAAAACCTTTGTCAAAATTAAGGCCTCACATAA CCTCAAGAAGAAGATCCTCCGCTTTCGGTCTGGCTCTTTGAAACTGATGACGACGGTTTGA >OriGene 5' read for NM_016651 unedited
CTCGAGGGCTTCTAGCCACCGTCCCCGCCAGCGCCGCGCCCCGCCACAGGGCGGCATGAG CCCACCCGCGGCCGCAGCCCTAGCGCCCTGCTCCTCCGCCTGGGCGGCCCGGCTGCGGTG ACGGCTCTCGCTGCCCGACTGGGGGCCATGAAGCCGAGTCCGGCCGGGACGGCGAAGGAG CTGGAGCCTCCGGCGCCGGCCCGAGGCGAGCAGCGCACGGCGGAGCCCGAGGGGCGCTGG CGGGAGAAGGGCGAGGCAGACACCGAGCGGCAGCGCACCCGGGAGCGGCAGGAGGCCACG CTGGCCGGGCTGGCGGAGCTGGAGTACCTGCGCCAGCGCCAAGAGCTGCTGGTCAGGGGC GCCCTGCGCGGCGCCGGGGGTGCGGGAGCCGCTGCGCCCCGCGCTGGGGAGCTACTGGGG GAGGCGGCGCAGCGCAGTCGCCTGGAGGAGAAGTTCTTGGAGGAGAACATCTTGCTGCTA AGAAAGCAATTGAACTGTTTGAGGCGAAGAGATGCTGGTTTGTTGAATCAGTTGCAAGAG CTTGACAAGCAGATAAGTGACCTGAGACTGGATGTAGAAAAGACATCTGAAGAGCACCTG GAGACAGACAGTCGGCCTAGCTCAAGGTTTTATGAGCTGAGTGATGGGGCTTCANGATCC CTTTNCAATTCCTCTAACTCNGNGGTCAANGAGTGGTTATNCAGTTGTCATTTCAGCACC TGCTTTTGCAGCCCTTGGAGGCACCNTGAGTCTTTAAAAGGGTGCCCCAATCTGCAGATG >OriGene 3' read for NM_016651 unedited
NNNAAATCACTTGNACCGCGCCCATCTANGATCGATTTTTTTTTTTTTTTTTTACAGGTG TAAACATCTGGTTTTATACCCAAATAACAAACTTAACAGCAGTAAAACAAATGGTTTATT ACTGCATATGTTATATTAAATTTACACAATGATATATAAAAACACATACTGTTTATATTA TATAGTAATTTAACATCAACAGGAGTATCAACACAAGTACTACTCATGCACAAAACATGC ATATATTGGTATACAAAAAGCAATTTTACACAATACTGTTTACCAAAAATTTTTTCTTAA AAAACAGCCCTTCCACATAGGATCAAAGGTCCAATCTGGACTGGATTGCACTAATATGTT CAGGTCAACGCTTCGGTGGCATAGCGCTCAGTGAGCAATTCTGGGATTGGAGTCATGCCC AAGGGTTACTTCATTAATAGTGAATGTCTCTGTGCCCAAGGACCAAAATTTTATCCCAAA ACTAAATCCTCCAAGGAATGAAAGGTTGGACTGGCTACAAATTAGGGAGGGAAAAGAAGG CATCTAAATGTTGGATAAACGGCCCTAGAAGGGGTTATTAGGAACAGGGCCTAGGCACCA AGTGTGGCAAAAGTTTTTGTGAATCCTTTTAAGGTCCCTATCGAGGCTGCTATCTCTGGA TTTAAAGTTTGGCCTCCAAGTCATGAATCTAGTAACAGTTCTGAGAATAAAACCGAATGG GGGTACATCTGAGAAATGTTGACTTCCTAAAAAAACTTTTGAATACAATGACTGCCCCTC ATACCTACTTCACACTATTGTAAGGGGTGGCCCCTCACCGGGAAACACCCCTGGGAAACC TGCCCCGGAAACTCAGGAAAAAATTAAAATCCAAAATTCCCACCCCCCGGACCTGGGGAT CCAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016651 |
| Insert Size | 2511 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016651.4, NP_057735.2 |
| RefSeq Size | 3775 bp |
| RefSeq ORF | 2511 bp |
| Locus ID | 51339 |
| UniProt ID | Q9NYF0 |
| Gene Summary | The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212564 | DACT1 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1 |
CNY 7,130.00 |
|
| RC212564L3 | Lenti-ORF clone of DACT1 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1 |
CNY 9,030.00 |
|
| RC212564L4 | Lenti-ORF clone of DACT1 (mGFP-tagged)-Human dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1 |
CNY 9,030.00 |
|
| RG212564 | DACT1 (tGFP-tagged) - Human dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1 |
CNY 8,336.00 |
