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SMPD4 (NM_017751) Human Untagged Clone
CAT＃: SC114022
SMPD4 (untagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 1
CNY 5,856.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NEDMABA; NEDMEBA; NET13; NSMASE-3; NSMASE3; SKNY |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_017751, the custom clone sequence may differ by one or more nucleotides
ATGACGACTTTCGGCGCCGTGGCGGAATGGCGGCTTCCATCTCTGAGGCGAGCGACGCTATGGATCCCAC AGTGGTTTGCTAAGAAGGCCATTTTCAACTCTCCACTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCA GCCCAGCTTTCTACTGGCTAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAGAC TTGGTTAAAGTCATTGAGGACTTTCCAGCAAAGGAGCTGCACACCATCTTCCCATGGCTGGTAGAAAGCA TTTTTGGCAGCCTAGATGGTGTCCTCGTTGGCTGGAACCTCCGCTGCTTACAGGGGCGCGTGAATCCTGT GGAGTACAGCATCGTGATGGAATTTCTCGACCCTGGTGGCCCAATGATGAAGTTGGTTTATAAGCTTCAA GCTGAAGACTATAAGTTCGACTTTCCTGTCTCCTACTTGCCTGGTCCTGTGAAGGCGTCCATCCAGGAGT GCATCCTCCCTGACAGTCCTCTGTACCACAACAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAA CTTGGCCCTGAATCCGTTCGAGTATTACATATTCTTCTTTGCCTTGAGCCTCATCACTCAGAAGCCACTT CCTGTGTCCCTCCACGTCCGTACTTCAGACTGTGCCTATTTCATCCTGGTGGACAGGTACCTGTCATGGT TCCTGCCCACCGAAGGCAGTGTGCCCCCACCACTCTCCTCCAGCCCAGGGGGGACCAGCCCCTCACCACC TCCCAGGACACCAGCCATACCCTTTGCTTCCTATGGCCTCCACCACACTAGCCTCCTAAAGCGACACATC TCTCATCAGACGTCTGTGAATGCAGACCCCGCCTCCCACGAGATCTGGAGGTCAGAAACTCTGCTCCAGG TTTTTGTTGAAATGTGGCTTCATCACTATTCCTTGGAGATGTATCAAAAAATGCAGTCCCCTCATGCCAA GGAGTCGTTCACGCCTACTGAGGAGCATGTGTTGGTGGTGCGCCTGCTGCTGAAGCACCTGCACGCCTTT GCCAACAGCCTGAAGCCAGAGCAGGCCTCACCCTCCGCCCACTCCCACGCCACCAGCCCCCTGGAGGAGT TCAAACGGGCTGCTGTCCCGAGGTTCGTCCAGCAGAAACTCTACCTCTTCTTGCAGCATTGCTTTGGCCA CTGGCCCCTGGACGCATCGTTCAGAGCTGTCCTGGAGATGTGGCTGAGCTACCTGCAGCCGTGGCGGTAC GCGCCTGACAAGCAGGCTCCGGGCAGCGACTCCCAGCCCCGGTGTGTGTCGGAGAAATGGGCACCCTTTG TCCAGGAGAACCTGCTGATGTACACCAAGTTGTTTGTGGGCTTTCTGAACCGCGCGCTCCGCACAGACCT GGTCAGCCCCAAGCACGCGCTCATGGTGTTCCGAGTGGCCAAAGTCTTTGCCCAGCCCAACCTGGCTGAG ATGATTCAGAAAGGTGAGCAGCTATTCCTGGAGCCAGAGCTGGTCATCCCCCACCGCCAGCACCGACTCT TCACGGCCCCCACATTCACTGGGAGCTTCCTGTCACCCTGGCCACCAGCGGTCACTGATGCCTCCTTCAA GGTGAAGAGCCACGTCTACAGCCTGGAGGGCCAGGACTGCAAGTACACCCCGATGTTTGGGCCCGAGGCC CGCACCCTGGTCCTGCGCCTCGCTCAGCTCATCACACAGGCCAAACACACAGCCAAGTCCATCTCCGACC AGTGTGCGGAGAGCCCGGCTGGCCACTCCTTCCTCTCATGGCTGGGCTTTAGCTCCATGGACACCAATGG CTCCTACACAGCCAACGACCTGGACGAGATGGGGCAAGACAGTGTCCGGAAGACAGATGAATACCTGGAG AAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCGCAGCTCAGGCAGTTCACACTCGCCT TGGGCACCACCCAGGATGAGAATGGAAAAAAGCAACTCCCCGACTGCATCGTGGGTGAGGACGGACTCAT CCTTACGCCCCTGGGGCGGTACCAGATCATCAATGGGCTGCGAAGGTTTGAAATTGAGTACCAGGGGGAC CCGGAGCTGCAGCCCATCCGGAGCTATGAGATCGCCAGCTTGGTCCGCACACTCTTTAGGCTGTCGTCTG CCATCAACCACAGATTTGCAGGACAGATGGCGGCTCTGTGTTCCCGGGATGACTTCCTCGGCAGCTTCTG TCGCTACCACCTCACAGAACCTGGGCTGGCCAGCAGGCACCTGCTGAGCCCTGTGGGGCGGAGGCAGGTG GCCGGCCACACCCGCGGCCCCAGGCTCAGCCTGCGCTTCCTGGGCAGTTACCGGACGCTGGTCTCGCTGC TGCTGGCCTTCTTCGTGGCCTCTCTGTTCTGCGTCGGGCCCCTCCCATGCACGCTGCTGCTCACCCTGGG CTATGTCCTCTACGCCTCTGCCATGACACTGCTGACCGAGCGGGGGAAGCTGCACCAGCCCTGA >OriGene 5' read for NM_017751 unedited
CCATTATGATACGACTCACTTAGGCGGCCGCGAATCGCACGAGGCGGAATGGCGGCTTCC ACCTCTGAAGCGGGCGACGCTATGGGTCCCACAGTGGTTTGCTAAGAAGGCCATTTTCAA CTCTCCATTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCAGCCCAGCTTTCTACTGGC CAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAGACTTGATTAA AGCCATTGAGGATTTTCCAGCAAAGGAGCTGCACACCATCTTCCCATGGCTGGCAGAGAG CATTTTCGGCAGCCTAGATGGTGTCCTCGTTGGCTGGAACCTCCGCTGCTTACAGGGGCG TGTGAATCCTGTGGAGTACAGCATCGTGATGGAATTTCTCGACACTGGGTAGTGGCCCAA TGATGAAGTTGGTTTATAAGCTTCAAGCTGAAGACTATAAGTTCGACTTTCCTGTCTCCT ACCTGCCTGGTCCTGTGAAGGCGTCCATCCAGGAGTGCATCCTCCCTGACAGTCCTCTGT ACCACAGCAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAACCTGGCCCTGAATC CATTCGAGTATTACATACTCTTCTTTGCCCTGAGCCTCATCACTCAGAAGCCACTCCCTG TGTCCCTTCACGTCCGTACTTTAGACTGTGCCTATTTCATCCTGGCGGACAGGCACCTGT CATGGTTCCTGCCCACCGAAGCTAGTGTGCCCTGCCACTCTCCTCCAGCCAGGGGGGACC AGCCCCTCACCGCCTCCCAAAAATGTATATGCGGGCTGGGAGTGGCGGCTGACCCCTGTA ATTCCACCCTTGGGCGGGCCCAGCGGACGGACCCCCTGGGCCACGATTCCAAACCGCCTG GCCAAATGTGAAACCCCTTCCTCAATACACCAAGTTC >OriGene 3' read for NM_017751 unedited
GTCTCATGTNACACGACGGNCACGGCAATTCTTAGAATACGAAGGTTTTTTCTTTTTTTT CTTTTTTTCTTTTTTTCTTTTTTTCTTTTACAAAACCAGGGAACAGTGTTTTATTCAGAA CCCATGGGGCGTTACAAGCTTATTTTTTGGCCACAGGCCCTAAAAGGCCTTTATTCTAAG TTTACATAAAAATACAAAGCCCAACTTTTACAATTTAAAAAACATTAAAGCAAACCCCCA AAACCCCACACCGAAAAACAAAGGTTGGGTTGGGAAATAACCACTGAAATGCTGTTCTCC CTCGAATGTCCCCTGGGGGGGGAATGAAGGGGAAAACCCCGGGCTGGTTTTTGTAAAAAA ATCTGAAGTTGTATATTTGGAATACTTAAGTACCACTGAACGGTGGCCATGTTTTTGACC ACTTGATGTTACTGAAGGGACAATGCTTCTGACCATTTTTGGCTGCAAAGCAAAAACTGG AAAAATCCGACCCCATCGACCCAGAAGTGTCCCCAACGCCTGAGGACCGGCCCCCATTTG TGACAAGGCACAGGGGTCCGCGATGTTGGCCACTGCTTTGGGGGATGGGCTCAATGGTTT GGGGCCTGCGGGGAACCACCTGGGCTCTGGGAGGGTTTTAGGCCTCCTGAAAATGGAATT CACGGCCCATTCTTGGAACAGCTGGGAAAACATGAAAAACGCCCCCCAGAGGGAAGAATC AGGAAAGCCACTCTTTGGTTTTCTCACACCTTTAAAAATTTTGATTCTTTCCTGGAGGGG AGACTTAACCCACCGGCCCTGGTCCCAACAAAGAAAGAGGAGGCGCCAAAACCCACTCTG GCTTACTTAAGGCCACCCAGTGGTGAGGGCCGGCCCAGGCAATTTTTGGGAGGTTGGTCC CAAACTTTGGTGAACAAAATACCACATAGGCCCCTGGAAAAAGGCCCCGGAAGATGGCTC TGGGCATGAATCTTTGGCAAAGGAAGGGAAAAGGCCGCCTTTAACTCTGGCTTGTAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017751 |
| Insert Size | 3410 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017751.1, NP_060221.1 |
| RefSeq Size | 3833 bp |
| RefSeq ORF | 2397 bp |
| Locus ID | 55627 |
| UniProt ID | Q9NXE4 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (1) lacks an alternate in-frame exon in the central coding region, compared to variant 2, resulting in an isoform (1) that is shorter than isoform 2. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon. While this start codon is well-conserved, it should be noted that other than human and chimp, all other species have indels that follow this start codon. Other primates and rodents have frameshifting indels, and thus cannot use this start codon. However, some mammalian species, including cow, pig and dog, contain additional compensating indels that bring them back in frame; these species could therefore use the start codon represented in this CCDS. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 39 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214161 | SMPD4 (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 1 |
CNY 8,840.00 |
|
| RC214161L3 | Lenti-ORF clone of SMPD4 (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 1 |
CNY 10,740.00 |
|
| RC214161L4 | Lenti-ORF clone of SMPD4 (mGFP-tagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 1 |
CNY 10,740.00 |
|
| RG214161 | SMPD4 (tGFP-tagged) - Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 1 |
CNY 9,690.00 |
|
| SC318320 | SMPD4 (untagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 1 |
CNY 16,250.00 |
