MARCHF5 (NM_017824) Human Untagged Clone
CAT#: SC113977
41338 (untagged)-Human membrane-associated ring finger (C3HC4) 5 (MARCH5)
CNY 2,400.00
CNY 6,270.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | MARCH-V; MARCH5; MITOL; RNF153 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC113977 sequence for NM_017824 edited (data generated by NextGen Sequencing)
ATGCCGGACCAAGCCCTACAGCAGATGCTGGACAGAAGTTGCTGGGTTTGTTTTGCTACT GATGAAGATGATAGAACAGCTGAATGGGTGAGACCATGCAGGTGCAGAGGATCTACAAAA TGGGTTCACCAGGCCTGTCTACAACGCTGGGTGGATGAAAAGCAAAGAGGAAACAGTACA GCCAGAGTGGCATGTCCTCAGTGCAATGCTGAATACCTAATAGTTTTTCCAAAATTGGGT CCAGTGGTTTACGTCTTGGATCTTGCAGATAGACTGATCTCAAAAGCCTGTCCATTTGCT GCAGCAGGAATAATGGTCGGCTCTATCTATTGGACAGCTGTGACTTATGGAGCAGTGACA GTGATGCAGGTTGTAGGTCATAAAGAAGGTCTGGATGTTATGGAGAGAGCTGATCCTTTA TTCCTTTTAATTGGACTTCCTACTATTCCTGTCATGCTGATATTAGGCAAGATGATTCGC TGGGAGGACTATGTGCTTAGACTGTGGCGCAAATACTCGAATAAACTACAAATTTTAAAT AGTATATTTCCAGGGATAGGTTGTCCTGTTCCTCGAATTCCAGCTGAGGCCAATCCTTTA GCAGATCATGTCTCTGCTACTCGAATCTTGTGTGGAGCCCTTGTCTTTCCTACTATTGCT ACAATAGTTGGTAAATTGATGTTCAGTAGTGTTAACTCTAATTTACAAAGGACAATCTTG GGTGGAATTGCGTTTGTTGCCATAAAAGGAGCATTTAAAGTTTACTTCAAACAGCAGCAA TATTTACGACAGGCACACCGCAAAATTCTGAATTATCCAGAACAAGAAGAAGCATAA Clone variation with respect to NM_017824.4 >OriGene 5' read for NM_017824 unedited
AACCGCGATTTTGTAAACCCCGACTTCACTATNAGGGCGGCACGCGCAATTCGGCACGAG GCCGGCAGCAACTCGGCGCCCGCGGTCCATGGACCGGAACCTCGGGCCGACGGACGGGAA CCCGGGCCGCGATCGCCGCCTCCCCGCCTCAGGCTCCTCCTCCTCGCTCTCCGCCGCCTC CGCCGGACTCCCGCAGGCCCTGCACCGCCGCCGCCAGGCTAGCTTTATCTGCCCCGGGAA GCTGGGTGACGGGTTCGCGGCTGCCGCCGGACTGCGGCCTACTCCGCCGCCTCTCAGTGC TATTGTCCCTGGGCCTGGCCTTGAGCGGGTCCACTGGGGAAGGCCGTGTGCGCCGGCTCC GCGGAAGATGCCGGACCAAGCCCTACAGCAGATGCTGGACAGAAGTTGCTGGGTTTGTTT TGCTACTGATGAAGATGATAGAACAGCTGAATGGGTGAGACCATGCAGGTGCAGAGGATC TACAAAATGGGTTCACCAGGCCTGTCTACAACGCTGGGTGGATGAAAAGCANAGAGGAAA CAGTACAGCCAGAGTGGCATGTCCTCAGTGCAATGCTGAATACCTAATAGTTTTTCCAAA ATTGGGTCCAGTGGTTTACGTCTTGGATCTTGCAGATAGACTGATCTCAAAAGCCTGTCC ATTTGCTGCAGCAGGAATAATGGTCGGCTCTATCTATTGGACAGCTGTGACTTATGGAGC AGTGACAGTGATGCAGGTTGTAAGTCATAAAAAAGGTCTGGATGTTATGGAGAGAGCTGA TCCTTTATTCCTTTTAATTGGACTTCCTACTATTCCTGTCATGCTGATATTAAGCAAGAT GATTCGCTGGGAGGACTATGTGCTTAGACTGTGGCGCNATACTCCGAATAACTACAAATT TTAATAGTATATTTCCAGGGATAGGTTGTCCCGGTCCTCGAATCN >OriGene 3' read for NM_017824 unedited
CCGCGGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGAGAGTAAAATAACTTT ATTTTTATTTTTGCAAATCATCCTCTGCTCAAACTACTATAAACTGGCAGCTGTAATTTC TAGAAATATGGGTTGAAAAAACTCCCAGAGATGGAAGGACCTGATGAAACACTGAAACAC ACAAATACACACACACACACACACACACACACACACACACACGACTGCATGAGTCAAAAT TACTTCATGAAGTATGAGATGTCAACATTATGTAAATAAAATTTAATATTGAAAAAGTGA TCTGGAAGCCCTGTGAATTTTTACCCTACACATTTTGGAGGACATCACATGGGTTAAAGA AAACACCACAAAATCTCATTTGTTCAGATAATCATTTGAATGTTTACATACATTTTTGGT TATTTATTTATAACTAATTTAATTTACATTTCCTCATATTAACATATCCTCTTATTTAGT ATGTAATCAAATTATGTCAGTGAGAAAAATCCTCATAGTACCTTGAGTTGCTGATTCTGA GAAAACACTAAACATGAACATGTACAAAAGAGGCATTTGAATAAAACAGCATGTGCATTT TTTTTAATATTTGCACAGTTTGATCTTTGCATTTCTGTCTGGAGAAAATATGAACAAAAC CCAGGTTTACCATGCTAAGATAATGGTTCATATGACTGCCATANNAGTAGTACATACAAA CAATGAANNACAATCACATCCTTAAGTTCCATGATTACCGAGATGATTTCCCCTGCCCTA AGTGCTTTATTATATTTCTTAAAAATATANGAGCAGCAGCTTATCACAAGTCTNCACAAG TGCATTTATGATGGNATCANNNACACACACAGATTCTAGGATGAGAGACTGGCGACACCA GAANCAGTTTATGCTTCTCCTGTTCTGATAATCAGAATTTGCGGGTGCC |
Restriction Sites | NotI-NotI |
ACCN | NM_017824 |
Insert Size | 1960 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_017824.4, NP_060294.1 |
RefSeq Size | 3941 bp |
RefSeq ORF | 837 bp |
Locus ID | 54708 |
UniProt ID | Q9NX47 |
Domains | RING |
Protein Families | Transmembrane |
Gene Summary | MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215645 | MARCH5 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 5 (MARCH5) |
CNY 2,400.00 |
|
RC215645L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 5 (MARCH5), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215645L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 5 (MARCH5), mGFP tagged |
CNY 5,890.00 |
|
RG215645 | 41338 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 5 (MARCH5) |
CNY 4,000.00 |
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