Mitofusin 1 (MFN1) (NM_017927) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp762F247; FLJ20693; hfzo1; hfzo1, hfzo2, FLJ20693, MGC41806, DKFZp762F247; hfzo2; MGC41806; mitochondrial transmembrane GTPase Fzo-1; mitochondrial transmembrane GTPase FZO-2; mitofusin 1; putative transmembrane GTPase
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_017927, the custom clone sequence may differ by one or more nucleotides ATGGCAGAACCTGTTTCTCCACTGAAGCACTTTGTGCTGGCTAAGAAGGCGATTACTGCAATCTTTGACC AGTTACTGGAGTTTGTTACTGAAGGATCACATTTTGTTGAAGCAACATATAAGAATCCGGAACTTGATCG AATAGCCACTGAAGATGATCTGGTAGAAATGCAAGGATATAAAGACAAGCTTTCCATCATTGGTGAGGTG CTATCTCGGAGACACATGAAGGTGGCATTTTTTGGCAGGACAAGCAGTGGGAAGAGCTCTGTTATCAATG CAATGTTGTGGGATAAAGTTCTCCCTAGTGGGATTGGCCATATAACCAATTGCTTCCTAAGTGTTGAAGG AACTGATGGAGATAAAGCCTATCTTATGACAGAAGGATCAGATGAAAAAAAGAGTGTGAAGACAGTTAAT CAACTGGCCCATGCCCTTCACATGGACAAAGATTTGAAAGCTGGCTGTCTTGTACGTGTGTTTTGGCCAA AAGCAAAATGTGCCCTCTTGAGAGATGACCTGGTGTTAGTAGACAGTCCAGGCACAGATGTCACTACAGA GCTGGATAGCTGGATTGATAAGTTTTGCCTAGATGCTGATGTCTTTGTTTTGGTCGCAAACTCTGAATCA ACACTAATGAATACGGAAAAACACTTTTTTCACAAGGTGAATGAGCGGCTTTCCAAGCCTAATATTTTCA TTCTCAATAATCGTTGGGATGCCTCTGCATCAGAGCCAGAATATATGGAAGACGTACGCAGACAGCACAT GGAAAGATGCCTGCATTTCTTGGTGGAGGAGCTCAAAGTTGTAAATGCTTTAGAAGCACAGAATCGTATC TTCTTTGTTTCAGCAAAGGAAGTTCTTAGTGCTAGAAAGCAAAAAGCACAGGGGATGCCAGAAAGTGGTG TGGCACTTGCTGAAGGATTTCATGCAAGATTACAGGAATTTCAGAATTTTGAACAAATCTTTGAGGAGTG TATCTCGCAGTCAGCAGTGAAAACAAAGTTCGAACAGCACACTATCAGAGCTAAACAGATACTAGCTACT GTGAAAAACATAATGGATTCAGTAAACCTGGCAGCTGAAGATAAAAGGTTTCATGTGCAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_017927 unedited NGTAACGTCAAAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTTTA ACTTCTCGGGAAGATGAGGCAGTTTGGCATCTGTGGCCGAGTTGCTGTTGCCGGGTGATA GTTGGAGCGGAGACTTAGCATAATGGCAGAACCTGTTTCTCCACTGAAGCACTTTGTGCT GGCTAAGAAGGCGATTACTGCAATCTTTGACCAGTTACTGGAGTTTGTTACTGAAGGATC ACATTTTGTTGAAGCAACATATAAGAATCCGGAACTTGATCGAATAGCCACTGAAGATGA TCTGGTAGAAATGCAAGGATATAAAGACAAGCTTTCCATCATTGGTGAGGTGCTATCTCG GAGACACATGAAGGTGGCATTTTTTGGCAGGACAAGCAGTGGGAAGAGCTCTGTTATCAA TGCAATGTTGTGGGATAAAGTTCTCCCTAGTGGGATTGGCCATATAACCAATTGCTTCCT AAGTGTTGAAGGAACTGATGGAGATAAAGCCTATCTTATGACAGAAGGATCAGATGAAAA AAAGAGTGTGAAGACAGTTAATCAACTGGCCCATGCCCTTCACATGGACAAAGATTTGAA AGCTGGCTGTCTTGTACGTGTGTTTTGGCCAAAAGCAAAATGTGCCCTCTTGAGAGATGA CCTGGTGTTAGTAGACAGTCCAGGCACAGATGTCACTACAGAGCTGGATAGCTGGATTGA TAAGTTTTGCCTAGATGCTGATGTCTTTGTTTTGGTCGCAAACTCTGAATCAACACTAAT GAATACNGAAAAACACTTTTTTCACAAGGTGAATNGAGCGCTTTCCAAGCCCTATATTTT CATTCTCATAATCGTTGGGATGCCTCTGCATCAGAGCCAGATATATC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_017927 unedited GCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCATTAAGGTAAAAAAACAATAAT AATTCTTTAATGGAAATCCTGGAAAAATTCATACAAATAAAATTAAAAGCCTCCAATGGG GTATAATCCAGCAATATCCTAGGCAAATGCCTCCTGAAGAACAACAGCCTTTTTTAAAAC ATCACTGTTTATCATTCAAAATTCAAACGTCTCCTATCTTTGGCTATTTTTATCTCTTCA AACTTTACTCCTAAAATTGCACTAGGAAAGCGTTCCTTATTGGAATCATAATGGGAGCAA AGCTGAAAATGTTTCTTTAGAAACATCTAAGGAAATTACTTAGACCCAGATACTTATAAG TGACTATAACAAGCCTTTATCCTGTTTTACATCAGTTTTAACGGGAATAAAAAGTAAAAA CACATTTATTAGTGTTTCACATAGCTGCCATGATGGCAACGTGACTGTGACTTGCATAAA TAATACACTCAAGGACATTCCACACTCCTTGGTGGTTCCAGCAATGCGATTTACTAAATA AAAAAGTGACTATTCCCATGAAAGAAAGCATCAAAAACTAGTTTTGAGAAAGAAGATGAA AGGCTCAATCTCTAACTCCTGGATGCCAGGAAGCAGAGTTTTTCAGAATCAAAAACAAAT TAAGAGCTCACAGCCCCACCCCTCAGGGGTCACATAAGTTGGTACTGTTGCTTGTAACTA ATTCATGATTTTAGCCTTTTCTTTACTCAAAATTAAAACTAAGGACATAACACTCTTCAA AATACACATACCCTGAGACAGATCATTACCAGAATCTACACAGGGCTTTGCTATTTAGGT ACAGTTAGTACAATTCATATTTAAAGTAATTTCATAAAAATAACAACTGGTCCAATNNCT ACAGTTNCGNTNCCTCCTATCATGGCACCAAGCATCTCTATGGTAGGATNCTNCATGCTC GAAGTAGAAACTGCTATAAAATCTCACTCATTTCAAGTGACAGCTTT
Restriction Sites	NotI-NotI
ACCN	NM_017927
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017927.2, NP_060397.2
RefSeq Size	3400 bp
RefSeq ORF	1113 bp
Locus ID	55669
Gene Summary	The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks a 127 nt exon as compared to variant 1, resulting in a frameshift and use of an earlier stop codon. Isoform 2 is 371 aa shorter than isoform 1.

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