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Rsafd1 (TYW1) (NM_018264) Human Untagged Clone
CAT＃: SC113583
TYW1 (untagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1)
CNY 5,368.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RSAFD1; TYW1A; YPL207W |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113583 sequence for NM_018264 edited (data generated by NextGen Sequencing)
ATGGATCCTTCTGCGGATACATGGGACCTCTTCTCACCTTTAATATCATTATGGATAAAC AGGTTTTACATTTATTTGGGCTTTGCTGTTAGCATTAGCCTTTGGATTTGTGTCCAGATT GTCATCAAGACGCAGGGCAAGAACTTACAGGAAAAATCTGTTCCAAAAGCAGCTCAGGAT TTGATGACAAATGGTTATGTCTCCCTTCAAGAGAAAGACATCTTTGTGTCTGGAGTGAAG ATTTTTTATGGTTCTCAGACTGGAACAGCGAAGGGATTCGCAACAGTTCTTGCTGAAGCA GTTACATCCCTGGATCTGCCTGTGGCCATTATTAATCTAAAAGAATATGATCCAGATGAT CATCTGATAGAAGAGGTGACTAGTAAAAATGTCTGTGTCTTCCTGGTTGCGACATACACT GACGGCCTACCAACTGAAAGTGCAGAGTGGTTCTGCAAATGGTTAGAGGAAGCATCCATT GATTTTCGATTTGGCAAAACTTACCTGAAGGGTATGAGATATGCGGTATTTGGCCTGGGA AATTCTGCCTATGCTAGCCACTTCAACAAGGTTGGCAAAAATGTTGACAAGTGGCTCTGG ATGCTTGGCGCGCATCGTGTGATGAGTCGAGGGGAGGGCGACTGCGACGTGGTTAAAAGC AAGCACGGCAGCATTGAGGCCGACTTCAGAGCATGGAAGACCAAGTTCATCTCCCAGCTG CAGGCACTTCAGAAAGGGGAGAGAAAGAAGTCCTGTGGCGGCCACTGCAAGAAAGGCAAA TGTGAATCTCACCAACATGGCTCAGAGGAGAGGGAGGAAGGATCTCATGAGCAGGATGAA TTGCATCATAGAGACACCGAGGAGGAAGAACCCTTTGAGAGCTCCAGTGAAGAAGAGTTT GGTGGTGAGGACCATCAGAGCCTAAATTCCATTGTTGATGTTGAAGATTTGGGCAAAATT ATGGATCATGTGAAGAAAGAAAAGAGAGAAAAGGAACAGCAGGAAGAGAAGTCTGGTTTG TTCAGGAACATGGGGAGGAATGAAGATGGTGAAAGAAGAGCTATGATAACTCCTGCTCTC CGAGAAGCCCTTACTAAACAAGGTTATCAGTTGATTGGGAGCCACTCGGGGGTGAAGCTT TGCAGGTGGACAAAGTCCATGCTCCGAGGGAGAGGAGGTTGTTACAAACACACATTCTAT GGAATTGAGAGCCATCGCTGCATGGAAACCACCCCGAGCTTGGCGTGTGCTAATAAATGT GTCTTCTGTTGGCGGCACCACACCAACCCCGTGGGCACTGAGTGGCGGTGGAAGATGGAC CAGCCTGAAATGATCTTGAAGGAAGCCATTGAAAACCATCAGAACATGATTAAGCAGTTT AAAGGAGTACCGGGCGTCAAAGCAGAACGCTTTGAAGAAGGAATGACGGTAAAGCACTGT GCATTGTCCCTCGTGGGAGAACCAATAATGTACCCAGAGATCAACAGGTTTTTGAAGCTA CTCCACCAGTGTAAAATTTCCAGCTTCCTGGTCACAAACGCACAATTTCCTGCGGAAATC AGGAACCTCGAGCCGGTTACTCAGCTGTATGTCAGTGTGGATGCCAGTACCAAAGACAGC CTGAAGAAAATCGACCGCCCACTCTTCAAGGATTTCTGGCAGAGATTCCTTGACAGTTTA AAAGCCTTGGCAGTCAAGCAACAACGAACTGTCTACAGACTGACGCTCGTGAAAGCATGG AACGTGGACGAGCTCCAGGCCTACGCGCAGCTCGTGTCCCTGGGGAATCCTGACTTCATC GAAGTGAAGGGCGTTACCTACTGCGGAGAAAGTTCAGCAAGCAGTCTTACCATGGCCCAC GTGCCCTGGCATGAGGAAGTGGTACAGTTTGTCCACGAGTTGGTGGATCTGATCCCCGAA TATGAAATTGCATGTGAACACGAACACTCTAATTGCCTCCTGATAGCACACAGAAAGTTT AAAATTGGTGGTGAATGGTGGACATGGATCGATTATAACCGCTTCCAGGAGCTCATCCAG GAATATGAAGATAGTGGTGGATCAAAAACGTTCAGCGCAAAGGATTATATGGCCAGAACT CCTCACTGGGCATTATTTGGTGCCAGTGAAAGAGGCTTTGATCCCAAGGACACAAGACAT CAGAGAAAGAACAAATCAAAGGCTATTTCTGGATGTTGA Clone variation with respect to NM_018264.2 >OriGene 5' read for NM_018264 unedited
GTTTGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGGTCATGGGC TGCCCACAGGTCTGCAGGCACTCGGTACGCCGCTAACGCGGCGAGGTAGCTCGGTGCGTC TCGCGGTACCAGTGCGAATCATCGGGCTATCCAGGTCCGAGATCCTAGTCTCCTGTCGGC TCTGAGGAGGATGGATCCTTCTGCGGATACATGGGACCTCTTCTCACCTTTAATATCATT ATGGATAAACAGGTTTTACATTTATTTGGGCTTTGCTGTTAGCATTAGCCTTTGGATTTG TGTCCAGATTGTCATCAAGACGCAGGGCAAGAACTTACAGGAAAAATCTGTTCCAAAAGC AGCTCAGGATTTGATGACAAATGGTTATGTCTCCCTTCAAGAGAAAGACATCTTTGTGTC TGGAGTGAAGATTTTTTATGGTTCTCAGACTGGAACAGCGAAGGGATTCGCAACAGTTCT TGCTGAAGCAGTTACATCCCTGGATCTGCCTGTGGCCATTATTAATCTAAAAGAATATGA TCCAGATGATCATCTGATAGAAGAGGTGACTAGTAAAAATGTCTGTGTCTTCCTGGTTGC GACATACACTGACGGCCTACCAACTGAAAGTGCAGAGTGGTTCTGCAAATGGTTAGAGGA AGCATCCATTGATTTTTCGATTTGGCAAAACTTACCCTGAGGGTATGAGATATGCGGCAT TTGGCCTGGGAAATTCTGCCTATGCTAGCCACTTCACCAAGGTTGGCAAAAATGTTGACC AGTGGGCTCTGGATGCTTTGCGCGCACTGTGTGATGACTCCAGGGAAGGGCGACTGCCAC CTGGTTAAAACCCGCCCGGCAGCATTGGAGCCGCTTTCAAACCTGGAAAACCAAGTTCAT CTCCAGCTGCAGCCCT >OriGene 3' read for NM_018264 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTACAAAAAATTATTTTATTA ATTCAATTTTTGCATAAAAGACTGTTAAAAGAACTTGCTAACTTGAAAACAATTTTGAGT ATTTATGATCTTTCCAACTTGAAAACACCTGAACCTTATAGAACAGATTGTGTAAAGCAG CCAGGGCCACAGGAGTGAAAGAAGATGGAGACCCGCCGTCTGGTCATGATATGAGAGGCC TGGACAGTGACCTCACGAACAAAAAGAAATGATCCTCTTCAGTCCAAAGTGTGTTTGTGA GACTAATGACTCCATGCCCTCACATGGCCACTCCTCTTACAGCAGACAGCTTCACTCCGC TCCAGCCCCGGTACTGCGGTCTGTGGTAATTTACATGGGAATGGGATGAGATCTAGTAGT TTTAGATCCAACGCAATTCTGGGAAGGGTTGGTAATAAACCAAAACTCAATCTATGCAGT ATTTAAAAAATAATTGAGAGTTGTGACAACTTCGATTCTTTTCAGGAGGTGCTGTCTTAA GATAGAAGAAAGGGATCAAGCTCTTATCTTAGAAAGCACAGACACGTTTAGCTCAGGGTA GTGCAATTCAATGCTAAGTGGCTGCTCCATGAAATCTAAGGGCCGGGTAAGGGGAAGAGG CCCAGGGACTTCTTCCTTGTCTGACACTCTCGGGACTAGCATTCTGGCAGGTCTTTACAA GTATAATACANAACCATCAGATAAATTCTAATCACGACTGTGTAGTTNCTTCCCAAAATA ATTTTCCTCTTTAGAAGTAAATCAGGAAAGGGGGCTGAGTCTGAAAAGAAACATCGGGCT GTGGCCCAGCCCTCTGAGTGNGACGCTGTCCCCGTGTCTCCAGTTTACTGGCTTATCGCT CCTTGNATGAAAGATAATTACGTAATTCGCCTTGGAGATACAGGGTTGTTCAGAACTTTT GAGGCCTTCAGTTTTTGNCTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018264 |
| Insert Size | 3300 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018264.2, NP_060734.2 |
| RefSeq Size | 3344 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 55253 |
| UniProt ID | Q9NV66 |
| Domains | flavodoxin, Radical_SAM |
| Protein Families | Transmembrane |
| Gene Summary | Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204851 | TYW1 (Myc-DDK-tagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) |
CNY 5,800.00 |
|
| RC204851L3 | Lenti-ORF clone of TYW1 (Myc-DDK-tagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) |
CNY 7,700.00 |
|
| RC204851L4 | Lenti-ORF clone of TYW1 (mGFP-tagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) |
CNY 7,700.00 |
|
| RG204851 | TYW1 (tGFP-tagged) - Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) |
CNY 6,370.00 |
