Macro H2A.2 (H2AFY2) (NM_018649) Human Untagged Clone
CAT#: SC113407
H2AFY2 (untagged)-Human H2A histone family, member Y2 (H2AFY2)
CNY 3,656.00
Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | H2AFY2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC113407 sequence for NM_018649 edited (data generated by NextGen Sequencing)
ATGTCGGGCCGGAGTGGGAAGAAGAAAATGTCCAAGCTGTCCCGTTCAGCTAGGGCAGGT GTCATCTTTCCAGTGGGGAGGCTGATGCGTTATCTGAAGAAAGGGACGTTCAAGTACCGG ATCAGCGTGGGCGCCCCTGTCTACATGGCGGCAGTCATTGAGTACCTGGCAGCGGAAATT CTAGAATTGGCCGGCAATGCCGCGAGGGACAACAAGAAGGCCCGGATAGCCCCGAGACAC ATCTTGCTGGCAGTTGCCAATGACGAGGAGCTCAACCAGCTGCTAAAAGGAGTGACCATC GCCAGTGGAGGCGTCCTGCCCAGAATTCACCCCGAACTGCTGGCCAAAAAGCGAGGGACC AAAGGCAAGTCGGAAACGATCCTCTCCCCACCCCCAGAGAAAAGAGGCAGGAAGGCCACG TCAGGCAAGAAGGGGGGGAAGAAATCCAAGGCTGCCAAACCACGGACGTCCAAAAAGTCC AAACCAAAGGACAGCGATAAAGAAGGAACTTCAAATTCCACCTCTGAAGATGGGCCAGGG GATGGATTCACCATTCTGTCTTCTAAGAGCCTTGTTCTGGGACAGAAGCTGTCCTTAACC CAGAGTGACATCAGCCATATTGGCTCCATGAGAGTGGAGGGCATTGTCCACCCAACCACA GCCGAAATTGACCTCAAAGAAGATATAGGTAAAGCCTTGGAAAAGGCTGGGGGAAAAGAG TTCTTGGAAACGGTAAAGGAGCTTCGCAAATCCCAAGGCCCTTTGGAAGTCGCCGAAGCC GCCGTCAGCCAATCCAGTGGACTCGCAGCCAAATTTGTCATCCACTGTCACATCCCTCAG TGGGGCTCCGACAAATGTGAAGAACAGCTTGAAGAGACCATCAAAAACTGCCTGTCAGCG GCGGAGGACAAGAAGCTAAAGTCCGTCGCGTTCCCGCCTTTCCCCAGCGGCAGAAACTGC TTTCCCAAACAGACTGCGGCCCAGGTGACCCTCAAAGCCATCTCAGCCCACTTTGATGAC TCGAGCGCGTCCTCGCTGAAGAACGTGTACTTCCTGCTCTTCGACAGCGAGAGCATCGGC ATCTACGTGCAGGAGATGGCCAAGCTCGACGCCAAGTAG Clone variation with respect to NM_018649.2 >OriGene 5' read for NM_018649 unedited
CATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGCTTGCCGCCA TTGACACGCACAGATAGAACCCAAAGAAAGGCAAAGAGTCCTGCCCGGCACCGGCGCCGC GTGGGCCAAACCTGCGCCCGTGGAGGGGCGCGCAGAGGGCACCGGGCGCCGGGAGCAGGC GGCGCAGCACCAGCATTGTGTTAGTGCCGGGAGGCCACTGTGTCAGCAAGCTGAGAGGGA AACTGAAGCAAGATGTCGGGCCGGAGTGGGAAGAAGAAAATGTCCAAGCTGTCCCGTTCA GCTAGGGCAGGTGTCATCTTTCCAGTGGGGAGGCTGATGCGTTATCTGAAGAAAGGGACG TTCAAGTACCGGATCAGCGTGGGCGCCCCTGTCTACATGGCGGCAGTCATTGAGTACCTG GCAGCGGAAATTCTAGAATTGGCCGGCAATGCCGCGAGGGACAACAAGAAGGCCCGGATA GCCCCGAGACACATCTTGCTGGCAGTTGCCAATGACGAGGAGCTCAACCAGCTGCTAAAA GGAGTGACCATCGCCAGTGGAGGCGTCCTGCCCAGAATTCACCCCGAACTGCTGGCCAAA AAGCGAGGGACCAAAGGCAAGTCGGAAACGATCCTCTCCCCACCCCCAGAGAAAAGAGGC AGGAAGGCCACGTCAGGCAAGAAGGGGGGGAAGAAATCCAAGGCTGCCAAACCACGGACG TCCAAAAAGTCCAAACCAAAGGACAGCGATAAAGAAGGAACTTCAAATTCCACCTCTGAA GATGGGCCAGGGGATGGNATCACCATTCTGTCTTCTAAGAGCTTGTTCTGGGACAGAAGC TGTCCTTACCCAGAGTGACATCAGCCATATGGNCTNCATGAGAGTGGNAGGGCATTGTNC ACCCAACCACAGCCCGAAATGACCTCCAAGAAGATATAAGTAAGCCTTGGAAAGGCTGNG GGAAAAGAGTCCTGGAACG >OriGene 3' read for NM_018649 unedited
GCAATCTAGAGTCGAGTCCCNCCCCTTTCCTTTCTTCTCTTTTTTTTTTTTTCTTTTCTT CCCCTTTTTTTTTTTTTCCTTAAAAAAACCACGGGATCTTTTATTCTTTTCCTTTCATTT TCTTTTCCTAAAAAAACAAAATGAAAAAAAAAAAACGCCTGAATCAAAACCTTTTTAGGA GTATTTACAGATTTTTTAGGGATGGGGGCGGGGGGCACTAAAACAAAAAAAAAAACCCCC AGGGAAATGTTTTTCCCATTTTTTTTTTTCCCCCCGGGAACACCCCCACCAACAAACCCC AGGCCCTTTTTTGCCCTTTTCCCTTGGAAAAAGGGGGAACAAAAAATTTAAAAATTTTGA AAGGATTTTTTTCTTGGGGAAAGGGGGGAAAAAGCAACTGCTATAAATCTAAACCGAGGT CTGTTTTTTTAAAAAAGTTCTAAACCACCGGGGGGAAACCCCCTGGAAAAAAACCCCTTG GGGAAACCTGTTTTTTTATAAATGGAGGTTTTTTTGAAGAAAAGTGGAAAGGGAAAAGGT CTCCTTGCCCCGGAAGGAACTGCCGGGACACCCTTCACTCCCTGGCAATAACCCCTTCTT TTTTTTTTTAAAAACAAAACCCCCCTTTTTGGAACTCCGGGCCCTCTCCCAACCCCTTGC TGAAAAAGGGGGGGGGCTTCCTTCGGCCGAAAACCTTGCCCTCTCCCTCGCCGAAAATGT CCAAGCTTTCCCCTTGTATAAAAAGAAAGAATCACACGTTTCTTTAGCAGGGACCCCGCT CAAATAATTAAAAGGGGCTTAAAATGGCTTTTAGGGTCACCCTGGCCCATATTTTGTTTG AGAAAACCATTTCTTCTCTTGTGGAAAGGCGAAACCGCACAGATTTTATTTTTTGCCCTC CCCGTACAAGAGATTTTTAGAGGTTCTCTTAACGTTTTCTACAATTGGCGGACCCCCCTT GGGGAGGGAACAGGTGAACACATTGCTTCAAACACCGTTTTGG |
Restriction Sites | NotI-NotI |
ACCN | NM_018649 |
Insert Size | 2000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018649.1, NP_061119.1 |
RefSeq Size | 1932 bp |
RefSeq ORF | 1119 bp |
Locus ID | 55506 |
UniProt ID | Q9P0M6 |
Domains | H2A, A1pp, histone |
Protein Pathways | Systemic lupus erythematosus |
Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation. [provided by RefSeq, Oct 2015] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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X Chromosome Inactivation and Differentiation Occur Readily in ES Cells Doubly-Deficient for MacroH2A1 and MacroH2A2
,null,
PLoS ONE
,PubMed ID 21738686
[MACROH2A2]
|
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