United States
Germany
Japan
United Kingdom
China
TRIB3 (NM_021158) Human Untagged Clone
CAT＃: SC112991
TRIB3 (untagged)-Human tribbles homolog 3 (Drosophila) (TRIB3)
CNY 3,656.00
| Cited in 4 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C20orf97; NIPK; SINK; SKIP3; TRB3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_021158 edited
ATGCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGTCCAGGAAGAAGCGGTTGGAG TTGGATGACAACTTAGATACCGAGCGTCCCGTCCAGAAACGAGCTCGAAGTGGGCCCCAG CCCAGACTGCCCCCCTGCCTGTTGCCCCTGAGCCCACCTACTGCTCCAGATCGTGCAACT GCTGTGGCCACTGCCTCCCGTCTTGGGCCCTATGTCCTCCTGGAGCCCGAGGAGGGCGGG CGGGCCTACCAGGCCCTGCACTGCCCTACAGGCACTGAGTATACCTGCAAGGTGTACCCC GTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTACGCGCGGCTGCCCCCGCACAAGCATGTG GCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGCTCCTCTACGCCTTTTTCACTCGGACC CATGGGGACATGCACAGCCTGGTGCGAAGCCGCCACCGTATCCCTGAGCCTGAGGCTGCC GTGCTCTTCCGCCAGATGGCCACCGCCCTGGCGCACTGTCACCAGCACGGTCTGGTCCTG CGTGATCTCAAGCTGTGTCGCTTTGTCTTCGCTGACCGTGAGAGGAAGAAGCTGGTGCTG GAGAACCTGGAGGACTCCTGCGTGCTGACTGGGCCAGATGATTCCCTGTGGGACAAGCAC GCGTGCCCAGCCTACGTGGGACCTGAGATACTCAGCTCACGGGCCTCATACTCGGGCAAG GCAGCCGATGTCTGGAGCCTGGGCGTGGCGCTCTTCACCATGCTGGCCGGCCACTACCCC TTCCAGGACTCGGAGCCTGTCCTGCTCTTCGGCAAGATCCGCCGCGGGGCCTACGCCTTG CCTGCAGGCCTCTCGGCCCCTGCCCGCTGTCTGGTTCGCTGCCTCCTTCGTCGGGAGCCA GCTGAACGGCTCACAGCCACAGGCATCCTCCTGCACCCCTGGCTGCGACAGGACCCGATG CCCTTAGCTCCAACCCGATCCCATCTCTGGGAGGCTGCCCAGGTGGTCCCTGATGGACTG GGGCTGGACGAAGCCAGGGAAGAGGAGGGAGACAGAGAAGTGGTTCTGTATGGCTAG >OriGene 5' read for NM_021158 unedited
NGGTTCACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGCGAT CCCGGGCCCGAGGGCATCAGACGGCGGCTGATTAGCTCCGGTTTGCATCACCCGGACCGG GGGATTAGCTCCGGTTTGCATCACCCGAACCGGGGGATTAGCTCCGGTTTGCATCACCCG GACCGGGGGCCGGGCGCGCACGAGACTCGCAGCGGAAGTGGAGGCGGCTCCGCGTGCGTC CGCTGCTAGGACCCGGGCAGGGCTGGAGCTGGGCTGGGATCCCGAGCTCGGCAGCAGCGC AGCGGGCCGGCCCACCTGCTGGTGCCCTGGAGGCTCTGAGCCCCGGCGGCGCCCGGGCCC ACGCGGAACGACGGGGCGAGATGCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGT CCAGGAAGAAGCGGTTGGAGTTGGATGACAACTTAGATACCGAGCGTCCCGTCCAGAAAC GAGCTCGAAGTGGGCCCCAGCCCAGACTGCCCCCCTGCCTGTTGCCCCTGAGCCCACCTA CTGCTCCAGATCGTGCAACTGCTGTGGCCACTGCCTCCCGTCTTGGGCCCTATGTCCTCC TGGAGCCCGAGGAGGGGCGGGCGGGCCTACCAGGCCCTGCACTGCCCTACAGGCACTGAG TATACCTGCAAGGTGTACCCCGTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTACGCGCGG CTGCCCCCGCACAAGCATGTGGCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGCTCCTC TACGCCTTTTTCACTCGGACCCATGGNGACATGCACAGCCTGGTGCGAAGCCGCCACCGT ATCCCTGAGCCTGAGGCTGCCGTGCTCTTCCGCCAGATGCCACCGCCCTGGCGCACTGTA CCAGCACCGTT >OriGene 3' read for NM_021158 unedited
AAGGCCGCACGTGAGAGTCGAGTTTTTTTCTTTTTTTTTTATTTCATAATTCTCCTTTAT TAGGCACAGGTAAACATACATACTCATGGTATCCAAAACCTAGAGTATGGACCTGGGATT GTGGACCCCAAGTGTCCCCAGAAGAGTCCCACCTGGGACTTTCCAGGTGGCCACAGGACA GACCCTGCCTAATCCTGTCCCTCAACCTTGGTGCTCAGGTCAGAAGCCCCATGGTTGACA GGCCTGGACCCTCATTCCAGAACAGTCTTGAGTTAGACAAGAACTAGCCTCATAGTTTGG ATTCTTATCTCTGGCCCAAATCCCAGGCTTAGGCCTGGAAGGAGAATCTCTTAATCAAGA GGACAGAGATGCTGGGAACACAGTTCCCAGAGTGAGCTGGGCTTGGGTCCTGAGTTGCAG GGGAGAGGCCTAGAGCCTTTGGCACAGGGATACCTCCCTTTCTCTGAAAAGGTACAAGAT ACAAAGGCAGCTGGTTTGTTTGTGAAAGGGATTGTCCTTGGACAGAGGTGCCTGAGCACC AGCATCAGTGGAGGCCGACACTGGTACAAAGTGTACCCAGTGTAGACAGTGACCTATGGT CATGGAAAAGTGCTGTCACCTCCAGGATGCCAGCTTGTCCTCTCTGCGAGCAGGGAACAT TGGCTCCTTTGCTCACTGCTGGGACTCCCAGCACCGGAAACAGGCCTTGGCACCTGATAA CACCCCAAGCAAGAACTGGCTGTGGTGTGGGACAAACCAGATGGGTCACCGGACACCCCC ACCAAGGCTCCTGCCCTTNTCCCCTTCTGGAAGGCCCTCGAAGTTTCGCCTCACCTCAAA GCCCGGAGAAGGGCTGGAGCCTCCCCCCAACCTCACCCCCTGTGGCCCCTAGCCTGCACT ACGCGTGTCCTAACATAAAAACCCTTTCCNTGGCCCCTCCCCTTCCTGGTTCGGACCACC CCAAACATAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021158 |
| Insert Size | 2210 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021158.3, NP_066981.2 |
| RefSeq Size | 2469 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 57761 |
| UniProt ID | Q96RU7 |
| Domains | pkinase, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1, also known as 1A) represents the longest transcript and encode the predominant isoform (1). |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cellular stress induces TRB3/USP9x-dependent Notch activation in cancer
,Izrailit, J;Jaiswal, A;Zheng, W;Moran, MF;Reedijk, M;,
Oncogene
,PubMed ID 27593927
[TRIB3]
|
|
High throughput kinase inhibitor screens reveal TRB3 and MAPK-ERK/TGFß pathways as fundamental Notch regulators in breast cancer
,Julia Izrailit, Hal K. Berman, Alessandro Datti, Jeffrey L. Wrana, and Michael Reedijk,
PNAS, Jan 2013; 110: 1714 - 1719.
[TRIB3]
|
|
Molecular basis for antagonism between PDGF and the TGF? family of signalling pathways by control of miR-24 expression
,null,
The EMBO Journal
,PubMed ID 20019669
[TRIB3]
|
|
Molecular basis for antagonism between PDGF and the TGFß family of signalling pathways by control of miR-24 expression
,Mun Chun Chan, Aaron C Hilyard, Connie Wu, Brandi N Davis, Nicholas S Hill, Ashish Lal, Judy Lieberman, Giorgio Lagna, Akiko Hata,
The EMBO Journal 29, 559-573 (17 December 2009) doi:10.1038/emboj.2009.370 Article
[TRIB3]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206687 | TRIB3 (Myc-DDK-tagged)-Human tribbles homolog 3 (Drosophila) (TRIB3) |
CNY 3,656.00 |
|
| RC206687L1 | Lenti ORF clone of Human tribbles homolog 3 (Drosophila) (TRIB3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206687L2 | Lenti ORF clone of Human tribbles homolog 3 (Drosophila) (TRIB3), mGFP tagged |
CNY 6,056.00 |
|
| RC206687L3 | Lenti ORF clone of Human tribbles homolog 3 (Drosophila) (TRIB3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206687L4 | Lenti ORF clone of Human tribbles homolog 3 (Drosophila) (TRIB3), mGFP tagged |
CNY 6,056.00 |
|
| RG206687 | TRIB3 (tGFP-tagged) - Human tribbles homolog 3 (Drosophila) (TRIB3) |
CNY 5,256.00 |
