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C20orf77 (RPRD1B) (NM_021215) Human Untagged Clone
CAT＃: SC112934
RPRD1B (untagged)-Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C20orf77; CREPT; dJ1057B20.2; K-H; Kub5-Hera; NET60 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112934 sequence for NM_021215 edited (data generated by NextGen Sequencing)
ATGTCCTCCTTCTCTGAGTCGGCGCTGGAGAAGAAGCTCTCGGAGCTGAGCAACTCTCAG CAGAGCGTGCAGACCCTGTCCCTTTGGCTCATCCACCACCGCAAGCACGCGGGACCCATC GTCTCCGTGTGGCACCGCGAGCTCCGCAAAGCCAAATCAAATAGAAAGCTTACTTTTCTG TATTTAGCGAATGATGTCATCCAAAACAGTAAAAGGAAAGGACCTGAATTCACTAGAGAA TTTGAATCTGTCCTTGTGGATGCTTTTTCTCATGTTGCCAGAGAGGCAGATGAAGGCTGT AAAAAACCTTTAGAAAGATTGCTGAACATCTGGCAAGAACGAAGTGTGTATGGCGGCGAG TTCATACAGCAGCTGAAGCTGTCTATGGAGGACTCCAAGAGCCCTCCCCCCAAAGCAACA GAAGAGAAGAAATCTCTGAAACGAACTTTTCAGCAAATTCAGGAGGAGGAGGATGACGAC TACCCTGGCAGCTACTCTCCTCAGGATCCTTCTGCAGGACCCCTCTTGACTGAGGAACTA ATCAAAGCTTTGCAGGATCTGGAAAATGCCGCATCAGGGGATGCTACTGTCCGACAGAAA ATTGCTTCTCTGCCCCAGGAAGTGCAAGATGTTTCTCTATTGGAAAAAATAACAGACAAA GAGGCAGCTGAACGTCTTTCAAAAACAGTAGATGAAGCATGTCTGTTACTAGCAGAATAT AACGGGCGCCTGGCAGCAGAACTGGAGGACCGTCGCCAGCTGGCTCGGATGTTGGTGGAG TATACCCAGAATCAGAAAGATGTTTTGTCGGAGAAGGAGAAAAAACTAGAGGAATACAAA CAGAAGCTTGCACGAGTAACCCAGGTCCGCAAGGAACTGAAATCCCATATTCAGAGCTTG CCAGACCTCTCACTGCTGCCCAACGTCACAGGGGGCTTAGCCCCCCTGCCCTCTGCTGGG GACCTGTTTTCAACTGACTAG Clone variation with respect to NM_021215.3 >OriGene 5' read for NM_021215 unedited
GGGTGTTCAAATATTTGTATACGACTTCACTATAGGGCGGCCGCGAATCGGCACGAGGCG GGGAACTACTCTCCTTGCCTCGCTCTGTCTCCTTCGAAGTGCTCTGCGCGAGGTTCAGAG CGGCCGCCGCCTCCAAAGGGACGGTTTTCTAGAGCTCCGACGCCTCTCGGTGCCCCTCTG CTCCGGCCCTTGCCCTTTGACCTCGCTCTCGCGGCAGGGTGAGAGGTCGGGTGGCCATCT TGTGGCGGCGGCGCGGGCGGCTGTTACTGCGGAGACCCATCCCCTCCCCCTTCTCGCACC CCTGGCAGTCTGTCAGTCGGTAAAAAGTCCCGCAGCCTGTCAGGTGAGGCCCCGGCCTCG TGCCGTCGCTCTTCCCGCCGCACTGGGCGGCCCAGGCCGCTCCCTGCCGGGCCTCACTGC CGCCACCATGTCCTCCTTCTCTGAGTCGGCGCTGGAGAAGAAGCTCTCGGAGCTGAGCAA CTCTCAGCAGAGCGTGCAGACCCTGTCCCTTTGGCTCATCCACCACCGCAAGCACGCGGG ACCCATCGTCTCCGTGTGGCACCGCGAGCTCCGCAAAGCCAAATCAAATAGAAAGCTTAC TTTTCTGTATTTAGCGAATGATGTCATCCAAAACAGTAAAAGGAAAGGACCTGAATTCAC TAGAGAATTTGAATCTGTCCTTGTGGATGCTTTTTCTCATGTTGCCAGAGAGGCAGATGA AAGCTGTAAAAAACCTTTAGAAAGATTGCTGAACATCTGGCAAGAACGAAGTGTGTATGG CCGGCGAGTTCATACAGCAGCTGAAGCTGTCTATTGCAGGACTCCAAGAGCCCTCCCCCC ANAGCACCAGAAAGAGAAGAAATCTCTGAAACGAACTTTTAGCCAATCAGGAGGGAGGAG GATGACGACTACCCTGCCAGCTACTCTCCT >OriGene 3' read for NM_021215 unedited
CGCGGCACGCTATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTAAACAACACCAT CTTTATTCAAAAATATATATCTATGAAACATTTCAAAATATACTGCTGCCGCCAACGACA GCCTATACTTCTAAATAGTCCTAAATTAATATACAAATTTAAGCTTTGAAAATATTCTTT AAGAAAAACAAACAAACAAAAACAAGGGAAGATTGAAGACTTTCTGAGGAATATGACCTA AAGGAAAGGTCCTTTGAGGCCTACAGATGCCCTAAGAAAGAAAAAACACAAAACCAAAAA CCACCCACCCCACCCACCAAGTCACCTCATACATACAAGGAGGAAAGTTCATTTAAATTA AACAAATGAAGTAAGCACACTTTGGGAGGATGTGGACAGCAGATTTAAGATTTAAAAAAC GAAAGCATTTCATCAAAACAAAAAACTAACTTCCTCACAGGAAGTCCACTATTAACAGGT ATTAAGAGGCCAACACATTCATTTACATAATTGCTACAGTTTCATTTCTTAACTTTTGCT GAATAATTATGCACCATTTGCTGGCAAAAAACTCAGTGGGATAAAACAGAGCAGTGGGCT AAATCCATCCGGCTTTATTTTCATCTAATTGATCAGAGCAAATATCTACAATCCTTTTTT GGCTGGATTGGGGCGGCTAATTCGCACTTCCTTGGAGAAGCCTTAAAGGTCCAAAAGGGC CTGCTCCAAACACCCTCCTCTAAAAGCCCCACCCCCGGCGGGCCGGGGATAGGTGCCGCC TCACCCTCCATTTCATTCAACACCTCCTCGCCTTTTTAAGTATCCACAGTACCAGAGACG TACACCTCTCGCCCAATCTACCCCGATGGCGACATACATCGCTACTTCACTATCCACCAC GAGCACTNTACTTCCATCCATATCTTTAAACACTTATTCTGATCTTTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021215 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021215.2, NP_067038.1 |
| RefSeq Size | 4219 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 58490 |
| UniProt ID | Q9NQG5 |
| Domains | RPR, DUF618 |
| Gene Summary | Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207481 | RPRD1B (Myc-DDK-tagged)-Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B) |
CNY 2,400.00 |
|
| RC207481L1 | Lenti ORF clone of Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207481L2 | Lenti ORF clone of Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B), mGFP tagged |
CNY 5,890.00 |
|
| RC207481L3 | Lenti ORF clone of Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207481L4 | Lenti ORF clone of Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B), mGFP tagged |
CNY 5,890.00 |
|
| RG207481 | RPRD1B (tGFP-tagged) - Human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B) |
CNY 4,000.00 |
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