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Cryptochrome I (CRY1) (NM_004075) Human Untagged Clone
CAT＃: SC112662
CRY1 (untagged)-Human cryptochrome 1 (photolyase-like) (CRY1)
CNY 6,552.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DSPD; PHLL1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112662 sequence for NM_004075 edited (data generated by NextGen Sequencing)
ATGGGGGTGAACGCCGTGCACTGGTTCCGAAAGGGGCTCCGGCTCCACGACAACCCCGCC CTGAAGGNGTGCATTCAGGGCGCCGACACCATCCGCTGCGTCTACATCCTGGACCCCTGG TTCGCCGGCTCCTCCAATGTGGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTTGAG GATCTTGATGCCAATCTACGAAAATTAAACTCCCGTCTGTTTGTGATTCGTGGACAACCA GCAGATGTGTTTCCCAGGCTTTTCAAGGAATGGAACATTACTAAACTTTCAATTGAGTAT GATTCTGAGCCCTTTGGAAAGGAACGAGACGCAGCTATTAAGAAACTGGCAACTGAAGCT GGAGTAGAAGTCATTGTAAGAATTTCACATACATTATATGACCTAGACAAGATCATAGAA CTCAATGGTGGACAACCGCCTCTAACTTATAAAAGATTCCAGACTCTCATCAGCAAAATG GAACCACTAGAGATACCAGTAGAGACAATTACTTCAGAAGTGATAGAAAAGTGCACAACT CCTCTGTCTGATGACCATGATGAGAAATATGGAGTCCCTTCACTGGAAGAGCTAGGTTTT GATACAGATGGCTTATCCTCTGCAGTGTGGCCAGGCGGAGAAACTGAAGCACTTACTCGT TTGGAAAGGCATTTGGAAAGAAAAGCTTGGGTGGCAAATTTTGAAAGACCTCGAATGAAT GCGAATTCTCTGCTTGCAAGCCCTACTGGACTTAGTCCTTATCTCCGATTTGGTTGTTTG TCATGTCGACTGTTTTACTTCAAACTAACAGATCTCTACAAAAAGGTAAAGAAGAACAGT TCCCCTCCCCTTTCCCTTTATGGGCAACTGTTATGGCGTGAATTTTTCTATACAGCAGCA ACAAATAATCCACGCTTTGATAAAATGGAAGGAAACCCTATCTGTGTTCAGATTCCTTGG GATAAAAATCCTGAGGCTTTAGCCAAATGGGCGGAAGGCCGGACAGGCTTTCCATGGATT GATGCCATCATGACACAGCTTCGTCAGGAGGGTTGGATTCATCATCTAGCCAGGCATGCA GTTGCTTGCTTCCTGACACGAGGGGACCTGTGGATTAGTTGGGAAGAAGGAATGAAGGTA TTTGAAGAATTATTGCTTGATGCAGATTGGAGCATAAATGCTGGAAGTTGGATGTGGCTG TCTTGTAGTTCCTTTTTTCAACAGTTTTTTCACTGCTATTGCCCTGTTGGTTTTGGTAGG AGAACAGATCCCAATGGAGACTATATCAGGCGTTATTTGCCTGTCCTAAGAGGCTTCCCT GCAAAATATATCTATGATCCCTGGAATGCACCAGAAGGTATCCAAAAGGTAGCCAAATGT TTGATAGGAGTTAATTATCCTAAACCAATGGTGAACCATGCTGAGGCAAGCCGTTTGAAT ATCGAAAGGATGAAACAGATCTATCAGCAGCTTTCACGATATAGAGGACTAGGTCTTCTG GCATCAGTACCTTCTAATCCTAATGGGAATGGAGGCTTCATGGGATATTCTGCAGAAAAT ATCCCAGGTTGTAGCAGCAGTGGAAGTTGCTCTCAAGGGAGTGGTATTTTACACTATGCT CATGGCGACAGTCAGCAAACTCACCTGTTGAAGCAAGGAAGAAGCTCCATGGGCACTGGT CTCAGTGGTGGGAAACGTCCTAGTCAGGAAGAGGACACACAGAGTATTGGTCCTAAAGTC CAGAGACAGAGCACTAATTAG Clone variation with respect to NM_004075.3 68 a=>n;636 t=>c >OriGene 5' read for NM_004075 unedited
ATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTTTTTCCTGTTC CCCCTCTGCCGCCCTTTCCTCACGCCCCGGGTGAGGCAATTCTCTTGGAAGCGAAGGTGT CGGCTATGAGCCGGAGCCTCCTTCCTTGAATTTCTCCGTGGAGGACCCGCCGCGCCCCCC GGCATGGGGGTGAACGCCGTGCACTGGTTCCGAAAGGGGCTCCGGCTCCACGACAACCCC GCCCTGAAGGAGTGCATTCAGGGCGCCGACACCATCCGCTGCGTCTACATCCTGGACCCC TGGTTCGCCGGCTCCTCCAATGTGGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTT GAGGATCTTGATGCCAATCTACGAAAATTAAACTCCCGTCTGTTTGTGATTCGTGGACAA CCAGCAGATGTGTTTCCCAGGCTTTTCAAGGAATGGAACATTACTAAACTTTCAATTGAG TATGATTCTGAGCCCTTTGGAAAGGAACGAGACGCAGCTATTAAGAAACTGGCAACTGAA GCTGGAGTAGAAGTCATTGTAAGAATTTCACATACATTATATGACCTAGACAAGATCATA GAACTCAATGGTGGACAACCGCCTCTAACTTATAAAAGATTCCAGACTCTCATCAGCAAA ATGGAACCACTAGAGATACCAGTAGAGACAATTACTTCAGAAGTGATAGAAAAGTGCACA ACTCCTCTGTCTGATGACCATGATGAGAATATGGGAGTCCCTTCACTGGAGAGCTANGTT NTGAACAGATGGCTTNATCTCTGCAGTGTGGCCCAGCGGAGAACTGNAGCACTTACTCGT TTGGAAAAGCATTTGAAAGAAAGCTCGGTGGCAATTTTGAC >OriGene 3' read for NM_004075 unedited
GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATATTTGCATATACTTTAATAACAA GTTCATTTTAGTAATAAATGAACAAAGAAACAGAGACAGAGAACTTAACACATGATACTT CCACAAGTTTGGGTCTAAAAAGGTTTACATCAAAGTTTAAAACATATACTGTCTGTGTTA ACAGAGGCTACAATACCAATTTGGATTACGCACATTATCTAAAACATTATTATCAAAGAG TGCAAAGTTAGATCAAACAACATCAAGAAAATTCTGTCCTAAAATTTTAACGTCTTTTAA GAAAGTCTAAAAACCTCCTTTTTTCCCACTGACTTCAAAACTTTGAGTTTTATATCAAAA TATCAATTTGTCATTTTAACTTTACCATACATGAAAAATTATCAAAAATATATCGATGGG TCTATACTAATTGTGACTGTTTATATTTACATTAAGCAAATTCTCTTGCCAAGTTCTTTA TATACAAATTACCAATTGTACTCTGTGAAATTCATTAAAAAGTTAAAAACCACAGAAATG TCATTAGAAACAACATATTTCAATATGTAACTGCTTTCCATCAATGAAGAATATTTTTAA ATAACTTAATTGAAAAGTATTGAACTCCCCACCAATTTCAGCTGCAACAGTATTCCTCCT GAATGTTTTCTAATTAGTGCTCTGTCTCTGGACTTTANGACCAATACTCTGTGTGTCCTC TTCCTGACTAGGACGTTTCCCACCACTGAGACCAGTGCCCATGGAGCTTCTTNCTTGCTT CACANNGTGAGTTGCTGACTGTCCCATGAGCATAGTGAAAAACCACTCCCTTGAGAGCAC TTCCACTGCTGCTACACCTGGGATATTTCTGCAGATATCCCATGAGCCTCATTCCATTAG ATANAGGTACTGAGCCAAAGACTATCCTTTATTCGGAACTGTGATGATTGTTATCTTTCA TTAACGCTGCTACATGTACATGGTAGAAATACTCTAAACTTGCTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004075 |
| Insert Size | 2590 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004075.2, NP_004066.1 |
| RefSeq Size | 2999 bp |
| RefSeq ORF | 1761 bp |
| Locus ID | 1407 |
| UniProt ID | Q16526 |
| Domains | FAD_binding_7, DNA_photolyase |
| Protein Families | Druggable Genome |
| Protein Pathways | Circadian rhythm - mammal |
| Gene Summary | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207152 | CRY1 (Myc-DDK-tagged)-Human cryptochrome 1 (photolyase-like) (CRY1) |
CNY 4,360.00 |
|
| RC207152L1 | Lenti ORF clone of Human cryptochrome 1 (photolyase-like) (CRY1), Myc-DDK-tagged |
CNY 6,760.00 |
|
| RC207152L2 | Lenti ORF clone of Human cryptochrome 1 (photolyase-like) (CRY1), mGFP tagged |
CNY 6,460.00 |
|
| RC207152L3 | Lenti ORF clone of Human cryptochrome 1 (photolyase-like) (CRY1), Myc-DDK-tagged |
CNY 6,760.00 |
|
| RC207152L4 | Lenti ORF clone of Human cryptochrome 1 (photolyase-like) (CRY1), mGFP tagged |
CNY 6,760.00 |
|
| RG207152 | CRY1 (tGFP-tagged) - Human cryptochrome 1 (photolyase-like) (CRY1) |
CNY 5,960.00 |
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