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DAK (TKFC) (NM_015533) Human Untagged Clone
CAT＃: SC112650
DAK (untagged)-Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK)
CNY 4,288.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAK; NET45; TKFCD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112650 sequence for NM_015533 edited (data generated by NextGen Sequencing)
ATGACCTCCAAGAAGCTGGTGAACTCGGTGGCTGGCTGTGCTGATGACGCTCTTGCTGGC CTGGTGGCCTGCAACCCCAACCTGCAGCTCCTGCAGGGCCACCGCGTGGCCCTCCGTTCT GACCTGGACAGCCTCAAGGGCCGGGTGGCACTGCTGTCGGGTGGGGGCTCTGGCCATGAG CCTGCCCATGCTGGTTTCATAGGGAAGGGGATGCTGACTGGGGTCATCGCGGGAGCTGTG TTCACCTCCCCGGCAGTGGGCAGCATCCTGGCAGCCATCAGGGCCGTGGCCCAGGCCGGC ACAGTGGGGACGCTCCTTATCGTGAAGAACTACACTGGGGATCGGCTCAACTTCGGCCTG GCCCGGGAGCAGGCCCGGGCTGAAGGCATCCCGGTGGAGATGGTGGTGATTGGGGACGAC AGCGCCTTCACTGTCCTGAAGAAGGCAGGCCGGCGGGGGCTGTGCGGCACCGTGCTTATA CACAAGGTGGCAGGTGCTCTGGCTGAGGCTGGTGTGGGGCTGGAGGAGATCGCAAAGCAG GTGAACGTGGTCACCAAGGCCATGGGTACCCTGGGGGTGAGCTTATCCTCCTGCAGCGTC CCTGGTTCCAAACCCACCTTCGAGCTCTCAGCCGACGAGGTGGAGCTGGGCCTGGGGATC CACGGGGAAGCTGGTGTGCGCCGGATAAAGATGGCAACCGCCGATGAGATTGTGAAACTC ATGCTCGACCACATGACAAACACCACCAACGCGTCCCATGTGCCTGTGCAGCCCGGCTCC TCAGTTGTGATGATGGTCAACAACCTGGGTGGCCTGTCATTCCTGGAACTGGGCATCATA GCCGACGCTACCGTCCGCTCCCTGGAGGGCCGCGGGGTGAAGATTGCCCGTGCCCTGGTG GGCACCTTCATGTCAGCACTGGAGATGCCTGGCATTTCTCTCACCCTCCTGCTGGTGGAT GAGCCTCTCCTGAAACTGATAGATGCTGAAACCACTGCAGCAGCCTGGCCTAACGTGGCT GCAGTCTCCATTACTGGGCGGAAGCGGAGCCGGGTAGCCCCTGCCGAGCCCCAGGAGGCC CCTGATTCCACTGCTGCAGGAGGCTCAGCCTCGAAGCGGATGGCGCTGGTGCTGGAACGG GTGTGCAGCACTCTCCTGGGCCTGGAGGAACACCTGAATGCCCTGGACCGGGCTGCTGGT GACGGCGACTGTGGCACCACCCACAGCCGTGCGGCCAGAGCAATCCAGGAGTGGCTGAAG GAGGGCCCACCCCCTGCCAGCCCTGCCCAGCTGCTCTCCAAGTTGTCTGTTCTGCTCCTG GAGAAGATGGGAGGCTCATCTGGGGCGCTCTATGGCCTGTTCCTGACTGCGGCTGCACAG CCCCTGAAGGCCAAGACCAGCCTCCCAGCCTGGTCTGCTGCCATGGATGCCGGCCTGGAA GCCATGCAGAAGTATGGCAAGGCTGCTCCAGGGGACAGGACTATGCTGGATTCTCTGTGG GCAGCGGGGCAGGAGCTCCAAGCCTGGAAGAGCCCAGGAGCTGATCTGTTACAAGTCCTG ACCAAAGCAGTCAAGAGTGCCGAAGCTGCAGCCGAGGCCACCAAGAATATGGAAGCTGGA GCCGGAAGAGCCAGTTATATCAGCTCAGCACGGCTGGAGCAGCCAGACCCCGGGGCGGTG GCAGCTGCTGCCATCCTCCGGGCCATCTTGGAGGTCTTGCAGAGCTAG Clone variation with respect to NM_015533.3 471 g=>c;553 g=>a;1311 c=>t >OriGene 5' read for NM_015533 unedited
NTGTTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCGCACGCT TCGGGGTCTCCGGGNAGTCGCGGCGCCTTCGGATGTGGCGGATGCGGCCGTGAGCCGGCG GGGGAGGTGCTGCTGCTGCCTCCACTGTACTCAGACCCAGGTAGCACAGGATTGTCCATC CTCCAGCAGCTCAGTGCAACGGTGTGAACTCAGCCTGTTTCAGAGCCTCCACACCATGAC CTCCAAGAAGCTGGTGAACTCGGTGGCTGGCTGTGCTGATGACGCTCTTGCTGGCCTGGT GGCCTGCAACCCCAACCTGCAGCTCCTGCAGGGCCACCGCGTGGCCCTCCGTTCTGACCT GGACAGCCTCAAGGGCCGGGTGGCACTGCTGTCGGGTGGGGGCTCTGGCCATGAGCCTGC CCATGCTGGTTTCATAGGGAAGGGGATGCTGACTGGGGTCATCGCGGGAGCTGTGTTCAC CTCCCCGGCAGTGGGCAGCATCCTGGCAGCCATCAGGGCCGTGGCCCAGGCCGGCACAGT GGGGACGCTCCTTATCGTGAAGAACTACACTGGGGATCGGCTCAACTTCGGCCTGGCCCG GGAGCAGGCCCGGGCTGAAGGCATCCCGGTGGAGATGGTGGTGATTGGGGACGACAGCGC CTTCACTGTCCTGAAGAAGCAGGCCGGCGGNGGCTGTGCGGCACCGTGCTTATACACAAG GTGGCAGGTGCTCTGGCTGANNGCTGTGTGGNGCTGGNAGAGATCGCANAGCAGGTGAAC GTGGTCACCAAGCCATGGGTACCCTGNGGGTGAGCTNATTCTCCTGCAGCGTCCCTGGNT CCAAACCCACTTCGAGCTCTCAGCCGACGAGGGGGAGC >OriGene 3' read for NM_015533 unedited
CCGCGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTT CCATGTATTATAGTTTATTAACTTTCTTTTTCACGCTCACTGGGGTTAAGGGGCGGAAAG CATTTCACAGGGAGTCTTAAAACCAAATTAGGATTGCCAGGTAAAGGCTGCACAAGGAAA ATGCCTTATCTCTGAAGCCCAAAGCTGGCAGGGGAGGGCATGACCCAGGGACTCCACCCT CCTGCAAAGGGAAAGGGCATGCTGGAGAGGCATGGGGACCCACTCCAGCTCACTCTGGGT GCTGTCTGTAGTTCTGGAAGCTTGGTGGAGGATTTCCTGCTCAACTTAAAGGGTGGGCCA ATGGGGCCAGGCCGGGGGAAGGTGAGGGTTGGAAGGCAAAAGGAAGTGACAAAGGCCACC TCAACACAGCAGTGAGAGGAGCTGAGGCCAAGGGAGGCAGTCACACACCCTAGCTCTGCA AGACCTCCAAGATGGCCCGGAGGATGGCAGCAGCTGCCACCGCCCCGGGGTCTGGCTGCT CCAGCCGTGCTGAGCTGATATAACTGGCTCTTCCGGCTCCAGCTTCCATATTCTTGGTGG CCTCGGCTGCAGCTTCGGCACTCTTGACTGCTTTGGTCAGGACCTTGAACAGATCAGCTT CTGGGCTCTTCCAGCTTGGAGCTCCTGCCCCGCTGCCCCCAGAGAATCAGCATAATCCTG TCCCCTGGAGCAGCCCTGGCATATTTCTGCATGGGTTCCAAGCCCGCATTCCAGGCNAGC AGACCCGGCTGGGGAGGCTGGTCTTGGCCTTCAAGGGCTGTGCACCCGCATTCAGGAACA CGCCTAAAACGCCCCCATGAACCCTCCATTTTTTCCGGAGCAGAAAAAACCTTGGAACCA CCTGCCAAGCCTGGAAGGGGGGGCCCCTCTTCAACACTCTGGG |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_015533 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015533.2, NP_056348.2 |
| RefSeq Size | 2210 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 26007 |
| UniProt ID | Q3LXA3 |
| Domains | Dak1, Dak2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways, RIG-I-like receptor signaling pathway |
| Gene Summary | This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2017] Transcript Variant: This variant (2), as well as variant 3, encodes isoform b. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201724 | DAK (Myc-DDK-tagged)-Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK) |
CNY 4,280.00 |
|
| RC201724L3 | Lenti ORF clone of Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201724L4 | Lenti ORF clone of Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK), mGFP tagged |
CNY 5,890.00 |
|
| RG201724 | DAK (tGFP-tagged) - Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK) |
CNY 5,880.00 |
|
| SC324128 | DAK (untagged)-Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK) |
CNY 4,288.00 |
