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RECK (NM_021111) Human Untagged Clone
CAT＃: SC112572
RECK (untagged)-Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK)
CNY 10,680.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ST15 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112572 sequence for NM_021111 edited (data generated by NextGen Sequencing)
ATGGCGACCGTCCGGGCCTCTCTGCGAGGTGCGCTGCTCCTTCTGCTGGCCGTGGCGGGG GTCGCGGAGGTGGCAGGGGGCCTGGCTCCGGGCAGTGCGGGTGCATTGTGTTGTAATCAT TCAAAGGATAACCAAATGTGCCGTGATGTATGTGAACAGATTTTCTCCTCAAAAAGTGAA TCCCGACTAAAACATCTGTTGCAGCGAGCCCCAGATTATTGCCCAGAGACAATGGTTGAA ATTTGGAATTGTATGAATTCATCTTTGCCAGGTGTGTTTAAGAAGTCTGATGGCTGGGTT GGCTTAGGCTGCTGTGAACTGGCTATTGCCTTGGAGTGTCGACAGGCATGCAAGCAGGCA TCTTCAAAGAATGATATTTCCAAAGTTTGCAGAAAAGAATATGAGAATGCTCTTTTCAGT TGCATTAGCAGAAATGAAATGGGCTCGGTTTGTTGCAGTTATGCAGGTCATCACACAAAC TGCCGAGAATACTGTCAAGCCATTTTTCGAACAGACTCTTCTCCTGGTCCATCTCAGATA AAAGCAGTGGAAAATTATTGCGCCTCTATTAGTCCACAATTAATACATTGTGTGAACAAT TATACTCAATCTTATCCAATGAGGAACCCAACGGATAGTTTATATTGCTGTGACAGAGCT GAAGACCATGCTTGCCAAAATGCCTGCAAGAGAATCCTGATGTCCAAGAAAACGGAAATG GAGATTGTTGATGGTCTCATCGAGGGTTGTAAGACCCAGCCCTTGCCTCAAGATCCTCTT TGGCAATGTTTTCTTGAAAGCTCACAATCTGTTCACCCTGGAGTCACTGTACACCCTCCT CCCTCTACAGGCCTCGATGGGGCTAAATTGCATTGTTGTTCTAAAGCAAACACTTCAACA TGTAGGGAACTCTGCACTAAACTTTACAGCATGAGCTGGGGCAATACACAGAGTTGGCAA GAGTTTGATCGCTTTTGTGAATATAATCCAGTGGAAGTGTCCATGTTGACCTGTTTAGCG GATGTCCGGGAACCTTGCCAGTTGGGCTGTAGAAACCTTACTTACTGTACTAATTTTAAC AACAGGCCAACAGAACTTTTCAGGAGTTGTAATGCACAGTCAGATCAAGGAGCCATGAAT GACATGAAGTTGTGGGAGAAAGGAAGCATAAAGATGCCATTTATCAATATACCTGTTCTT GATATTAAAAAGTGCCAGCCAGAGATGTGGAAAGCAATAGCTTGTTCACTGCAGATTAAA CCTTGTCATAGTAAATCTCGGGGAAGTATTATTTGCAAATCAGATTGTGTGGAGATTCTT AAAAAATGTGGAGACCAGAACAAATTCCCTGAAGACCACACAGCTGAAAGTATTTGTGAG CTTCTGTCACCTACAGATGATCTGAAGAATTGTATACCTTTGGATACATACCTCAGGCCA AGTACTTTAGGTAACATTGTAGAAGAAGTGACTCATCCCTGTAACCCAAATCCTTGCCCT GCCAATGAGCTCTGTGAAGTAAACCGAAAAGGATGTCCATCTGGAGATCCCTGTCTTCCA TACTTTTGTGTTCAAGGTTGCAAACTGGGAGAAGCTTCTGATTTCATTGTCCGTCAAGGG ACACTAATCCAGGTGCCATCATCTGCAGGGGAAGTTGGTTGTTATAAAATCTGTTCATGT GGACAAAGTGGACTCTTAGAAAACTGTATGGAAATGCACTGTATAGACCTCCAGAAGTCT TGTATTGTTGGAGGAAAAAGAAAAAGTCATGGAACATCCTTTAGTATTGACTGCAATGTC TGTTCTTGTTTTGCTGGCAATTTGGTGTGCTCTACCCGCCTTTGCCTCAGTGAGCACAGT TCAGAAGATGACCGTCGTACCTTCACAGGTCTGCCCTGTAACTGTGCAGATCAGTTTGTC CCTGTATGTGGGCAGAATGGGCGCACTTACCCCAGTGCCTGCATTGCTCGCTGTGTGGGC CTCCAAGACCATCAGTTTGAGTTTGGATCATGCATGTCAAAGGATCCATGTAATCCTAAT CCCTGCCAAAAAAACCAAAGGTGCATACCCAAACCACAGGTCTGCCTGACGACTTTTGAT AAATTTGGATGTAGCCAGTATGAGTGTGTACCAAGACAGCTCGCGTGTGACCAGGTCCAA GATCCTGTTTGTGACACAGACCACATGGAGCACAACAATCTCTGCACTTTATACCAAAGA GGAAAAAGCCTCTCTTACAAAGGTCCCTGCCAGCCCTTTTGCAGAGCAACCGAGCCCGTA TGTGGGCACAATGGTGAGACCTACAGCAGTGTGTGTGCTGCCTACTCGGATCGCGTGGCA GTCGATTACTATGGGGACTGCCAGGCCGTCGGAGTCCTCTCAGAGCACAGCTCCGTCGCC GAGTGTGCTTCTGTCAAGTGTCCTTCGCTCTTGGCAGCTGGATGCAAACCCATCATCCCA CCGGGTGCTTGTTGCCCATTATGTGCTGGGATGTTAAGAGTTTTATTTGACAAAGAAAAA CTGGATACTATTGCTAAGGTAACAAATAAAAAGCCAATAACAGTTCTGGAAATACTTCAG AAAATCCGCATGCACGTGTCTGTCCCACAGTGTGATGTGTTTGGATACTTCAGCATTGAA TCAGAAATTGTGATCCTGATCATTCCCGTCGATCACTATCCAAAAGCTCTGCAGATTGAA GCCTGCAATAAAGAAGCAGAGAAGATTGAGTCCCTTATCAACTCTGACAGCCCGACTTTG GCGTCCCATGTCCCTCTCTCTGCCCTCATCATTTCCCAGGTACAGGTCTCCAGCAGTGTG CCATCGGCCGGTGTCAGGGCCAGGCCTTCTTGCCACTCCCTCCTCCTTCCCCTCAGCTTG GGCCTTGCCTTGCACTTGCTCTGGACATATAACTGA Clone variation with respect to NM_021111.2 >OriGene 5' read for NM_021111 unedited
GGGTTCACAAATTATGTATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGG CGGCAGCGGCTGCGGCCAAGCTGGGTCCGAGCATCCCGCGGCTCTGGAGCCGCCCGGCCC GGACATGGCGACCGTCCGGGCCTCTCTGCGAGGTGCGCTGCTCCTTCTGCTGGCCGTGGC GGGGGTCGCGGAGGTGGCAGGGGGCCTGGCTCCGGGCAGTGCGGGTGCATTGTGTTGTAA TCATTCAAAGGATAACCAAATGTGCCGTGATGTATGTGAACAGATTTTCTCCTCAAAAAG TGAATCCCGACTAAAACATCTGTTGCAGCGAGCCCCAGATTATTGCCCAGAGACAATGGT TGAAATTTGGAATTGTATGAATTCATCTTTGCCAGGTGTGTTTAAGAAGTCTGATGGCTG GGTTGGCTTAGGCTGCTGTGAACTGGCTATTGCCTTGGAGTGTCGACAGGCATGCAAGCA GGCATCTTCAAAGAATGATATTTCCAAAGTTTGCAGAAAAGAATATGAGAATGCTCTTTT CAGTTGCATTAGCAGAAATGAAATGGGCTCGGTTTGTTGCAGTTATGCAGGTCATCACAC AAACTGCCGAGAATACTGTCAAGCCATTTTTCGAACAGACTCTTCTCCTGGTCCATCTCA GATAAAAGCAGTGGAAAATTATTGCGCCTCTATTAGTCCACAATTAATACATTGTGTGAA CAATTATACTCAATCTTATCCAATGANGAACCCAACGGATAGTTTATATTGCTGTGACAG AGCTGAAGACATGCTTGCCAAATGCCTGCAAGAGATCCTGATGTCCAAGAAACGGAATGA AGATTGTGAGGGCTCATCGAGGTTGTAAGACCAGCCCTTGCCTCAGACCTCTTTTGCATG TTTTCTGAAGCTCCCATCTGTCACCCTGTAT >OriGene 3' read for NM_021111 unedited
TGTACGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTTGATCATATAAAAATTA TTTTTATTAAAATAGAAAAGCTTTCCAGTATATTGCATTAAAGACATTTATACAGAAAAA AGGCAATTGCAACAAGCATGCTACATCAGCACTGACATATTCTGAAAGCTTTAAGTGCCC ATTTTTCAGCAACATTTTGGTATAATACGCATATCCCTATGTATTTCAGAGGGTACATAT AATACTGAAAAAATACTTCATGTAAAATCTAAGTTCATGTGCATACACACAAGAAGTATT ACATCAAATAGCTTATTTCAAACTGTGAAACACATTCAAAGTATTCAATTTCTTAGACAA CATGCATACATTTTAATACTCAGCTTGACACTTTTAAATATATAAACCACAATTCTTTAT ACTTCTATATCAAAAAAGAAAGTCAGACAGAACACTTATCTACATTGTGCATATCACAAA AAGCAAATGTGTTTTCACGAATGGAAAGCACTGGGTTAACACACATGATTGTCATTTTAC AAATATGGCTATTCACCTTCTTCATAATAAAATAGAAATATAATGTATTATTTACATGAA CTACACATTATTTCACTCTTTGGCAATTATATAAGTAGCAGCAATTGGTTTAAAGATTGA TGTTTAAAAACTCTACATTTAACTTGGATGAATACCGATGAATATATATCTGGCTAATAT AAAAATTGCATAATGCAAGATGCTTACTATGCATGTCACTTCTTCCTTTCACAAAAGTCA CAGACACCAATCCGTGACCAGTGAGCCCTGTGCCTACACATATAATATATGAGTGTCATT TATCAGCCTGCTTTGCATACACAGTAGTTGGGGTCTGAAATAATTCCTGTATCTAAGCAA ATTCGCTGATAATTACTATTTACATATTTCAAAGATGTTATCTATGGTCATATCCTTATG CCAAAGCCCTTACCTGGAACCTACT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021111 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021111.1, NP_066934.1 |
| RefSeq Size | 4414 bp |
| RefSeq ORF | 2916 bp |
| Locus ID | 8434 |
| UniProt ID | O95980 |
| Domains | kazal |
| Protein Families | Transmembrane |
| Gene Summary | The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
RECK regulated endoplasmic reticulum stress response and enhanced cisplatin-induced cell death in neuroblastoma cells
,Chen, Y;Tsai, YH;Tseng, SH;,
Surgery. 2013 Nov;154(5):968-79
,PubMed ID 24084596
[RECK]
|
|
MicroRNA-15a promotes neuroblastoma migration by targeting reversion-inducing cysteine-rich protein with Kazal motifs (RECK) and regulating matrix metalloproteinase-9 expression.
,null,
The FEBS journal
,PubMed ID 23176145
[RECK]
|
|
Keratinization-associated miR-7 and miR-21 Regulate Tumor Suppressor Reversion-inducing Cysteine-rich Protein with Kazal Motifs (RECK) in Oral Cancer
,Hyun Min Jung, Brittany L. Phillips, Rushi S. Patel, Donald M. Cohen, Andrew Jakymiw, William W. Kong, Jin Q. Cheng, and Edward K. L. Chan,
J. Biol. Chem., Aug 2012; 287: 29261 - 29272.
[RECK]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212174 | RECK (Myc-DDK-tagged)-Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK) |
CNY 10,664.00 |
|
| RC212174L1 | Lenti-ORF clone of RECK (Myc-DDK-tagged)-Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK) |
CNY 13,064.00 |
|
| RC212174L2 | Lenti-ORF clone of RECK (mGFP-tagged)-Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK) |
CNY 9,030.00 |
|
| RC212174L3 | Lenti-ORF clone of RECK (Myc-DDK-tagged)-Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK) |
CNY 9,030.00 |
|
| RC212174L4 | Lenti-ORF clone of RECK (mGFP-tagged)-Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK) |
CNY 9,030.00 |
|
| RG212174 | RECK (tGFP-tagged) - Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK) |
CNY 12,264.00 |
