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DUSP6 (NM_022652) Human Untagged Clone
CAT＃: SC112460
DUSP6 (untagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 2
CNY 2,400.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HH19; MKP3; PYST1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112460 sequence for NM_022652 edited (data generated by NextGen Sequencing)
ATGATAGATACGCTCAGACCCGTGCCCTTCGCGTCGGAAATGGCGATCAGCAAGACGGTG GCGTGGCTCAACGAGCAGCTGGAGCTGGGCAACGAGCGGCTGCTGCTGATGGACTGCCGG CCGCAGGAGCTATACGAGTCGTCGCACATCGAGTCGGCCATCAACGTGGCCATCCCGGGC ATCATGCTGCGGCGCCTGCAGAAGGGTAACCTGCCGGTGCGCGCGCTCTTCACGCGCGGC GAGGACCGGGACCGCTTCACCCGGCGCTGTGGCACCGACACAGTGGTGCTCTACGACGAG AGCAGCAGCGACTGGAACGAGAATACGGGCGGCGAGTCGTTGCTCGGGCTGCTGCTCAAG AAGCTCAAGGACGAGGGCTGCCGGGCGTTCTACCTGGAAGATGAAGCCCGGGGCAAGAAC TGTGGTGTCTTGGTACATTGCTTGGCTGGCATTAGCCGCTCAGTCACTGTGACTGTGGCT TACCTTATGCAGAAGCTCAATCTGTCGATGAACGATGCCTATGACATTGTCAAAATGAAA AAATCCAACATATCCCCTAACTTCAACTTCATGGGTCAGCTGCTGGACTTCGAGAGGACG CTGGGACTCAGCAGCCCATGTGACAACAGGGTTCCAGCACAGCAGCTGTATTTTACCACC CCTTCCAACCAGAATGTATACCAGGTGGACTCTCTGCAATCTACGTGA Clone variation with respect to NM_022652.2 340 g=>t >OriGene 5' read for NM_022652 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGAGGCTGAGTCC AAGAGATAGCAAATCGAGTCTTAAATAATCCGGGGAGAAAGACGCCCGGGTAGATTTGAG GTGCAGCCTTGGAGGGAGGGATTAGAAGCCGCTAGACTTTTTTTCCTCCCCTCTCAGTAG CACGGAGTCCGAATTAATTGGATTTCATTCACTGGGGAGGAACAAAAACTATCTGGGCAG CTTCATTGAGAGAGATTCATTGACACTAAGAGCCAGCGGCTGCAGCTGGGTGCAGAGAGA ACCTCCGGCTTTACTTCTGTCTCGTCTGCCCCAACCGCTAGCCTCGGCTTGGGTAAGGCG AGGCGGAATTAAACCCCGCTCCGAGAGCGGCAGCTTCGCGCGCGGTGCGCTCGGCCTATG CCTGCCCCGAGGGGCGTCTGGTAGGCACCCCGCCCTCTCCCGCAGCTCGACCCCCATGAT AGATACGCTCAGACCCGTGCCCTTCGCGTCGGAAATGGCGATCAGCAAGACGGTGGCGTG GCTCAACGAGCAGCTGGAGCTGGGCAACGAGCGGCTGCTGCTGATGGACTGCCGGCCGCA GGAGCTATACGAGTCGTCGCACATCGAGTCGGCCATCAACGTGGCCATCCCGGGCATCAT GCTGCGGCGCCTGCAGAAGGGTAAACCTGCCGTGCGCGCGCTCTTCACGCGCGGCGAGGG ACCGGACCGCTTCACCCGGCGCTGTGGCACCGACACAGTGGTGCTCTACGACGAGAGCAG CAGCGACTGGAACGAGAATACGGNNCGCGAGTCGTTGCTCGGGCTGCTGCTCAAAANCTC AAGACGAGGCTGCCGGCCGCTCTACCTGAAGGTGGGCTCAGTAAGTCCAAGCGAGTCTCC TGCATGCGAGACN >OriGene 3' read for NM_022652 unedited
GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTAGCCAAAATAGTTATT TATTAATAATTTAAGGTTTTACATTCTTATAATAAATTCCAGCTCTAAACTTTACACCAC GAACATAATGGAGAATTTAACTCTCCCTTCTTCACAATCAAATGCATTTCTCTTATCAAA TGGGTACCCATGCTCACACACACACACTTCCAGTTTTATACAAATTTTTTTAAAGGAAAG AAACCAACCCAAAGTATTGCATTTGAGGTGACACTCCCTGAAGAATTTTATACAGAGGTA ATATACTGTTTAGCACAGCTGAAGGTTTTTTTATTCTTTCTTTTTTTAAAGTGAGCCCAT GATTTGGTGTCTTTCCCAGATTATCCCCTTTGCGACAACACAAGCAAAAATATTTACAAA GGTAAGGCATAGTCAAACTACATAAAGAGAAAAAATCATGAGGAAAATACATGAAAAAGA CTAAAGACTTCGCCATAACAAGGTCTTAGTGATAATAGTGTCCGTAAAGATGTCATCAGA ATTGGTAAAGTCAAGCATGCTGCAAATATACCCCTTTGGATTAAAAAAGAGCACAATCTT CTTTTTTTGTTNTGTTCTGTCTTAAGAAGTGGCATACTGCTCTTTCTCCCTTTGGATAAT TTCTTTTAACCCCATCAAAGGAAAAACAAACCCATTCAAACAACCTGTCAAGTGATCCAA GATCAAAATTTACCATAATCAATGGAGGATCCCATTTTTTTTCCAACCGATCCCCAAATA CAAACTAAATCTCCTTTTGCCTCCCTCTTTCCAAACTGGACTATCTCTCTTGAAAACATT TATTGCCGGTCCAAATCCAAAGGAACCCCTTCAAAGCCCTAACAACAACGGCTCCACCTT CATTCGGGCCCACCCTAGCCCTGGGAGCTCCAATCCCACCCCTCCCGGTTACCTCACCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022652 |
| Insert Size | 2930 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022652.2, NP_073143.2 |
| RefSeq Size | 2404 bp |
| RefSeq ORF | 708 bp |
| Locus ID | 1848 |
| UniProt ID | Q16828 |
| Domains | DSPc, RHOD |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting shorter isoform (b) lacks an internal protein segment compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216245 | DUSP6 (Myc-DDK-tagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 2 |
CNY 3,990.00 |
|
| RC216245L3 | Lenti-ORF clone of DUSP6 (Myc-DDK-tagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 2 |
CNY 5,890.00 |
|
| RC216245L4 | Lenti-ORF clone of DUSP6 (mGFP-tagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 2 |
CNY 5,890.00 |
|
| RG216245 | DUSP6 (tGFP-tagged) - Human dual specificity phosphatase 6 (DUSP6), transcript variant 2 |
CNY 4,240.00 |
