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VPS16 (NM_022575) Human Untagged Clone
CAT＃: SC112452
VPS16 (untagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 1
CNY 6,152.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DYT30; hVPS16 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112452 sequence for NM_022575 edited (data generated by NextGen Sequencing)
ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATAT GAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCT GCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCT AGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTG TGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGCTGCTCTGTGTG CAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGC ATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACTGAGTTT GGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGT GACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGG ACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTAC CTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGC AGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCACTCTTCACAGACACA GGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAAC ATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCC GTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATCCAG TTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGGGTCCGCATCTTCTCC CGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGCGGCCAGCGAGGAAATCTTCAAAATT GCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAGTATGAGAAAGAGAGC CAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAGCTGACCCAGGCCGTG CAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATGCAGAAGAGTCTGCTC AGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCCGACAGCTTCGTGCAC ATGTGTCAGGACCTGCGTGTGCTCAATGCTGTTCGGGACTATCACATCGGGATCCCGCTC ACCTATAGCCAATATAAGCAGCTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGG AGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGC GTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGGATGTCTCAGAT GAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCC GACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTGGAG TATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTG GCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCAC CTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCC CTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTAC AATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCA GAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAG GCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAG CGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTT ACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGC ATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGG GAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTG GAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGT CCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTG GCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCC ACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAAGCCCAGAAGAAGTGA Clone variation with respect to NM_022575.2 >OriGene 5' read for NM_022575 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACCAGCCATGGACTGCTA CACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAG CATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGG GGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCC AGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGG ACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGG TGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGA AGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGT GGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACT CCGCCGGATGCCAGAGGTGCCAGGTCTGCANAGTGCACCCTCCTGCTGGACTGTGCTGTG CCANGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCA TGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACA GATGGCTGTCTNCTTCACCTACCGACACCTGNCACTCTTCACAGACACAGGCTACATCTG GATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAACATCCGGGCACC TTCCAAGCAGATGGTCTGGTGCACCCGTTCTCGTANCAAGGAAAAGGCCCGTGGTGGTTG CCC >OriGene 3' read for NM_022575 unedited
TTATTNTGCTACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAGTGTT CCAACTTTATTTAAAACCAGACAGCCCTGCTACCATGCCCCCGCTCCTCAGCATTGCTCT AGGGGAGGAGCTGGATGAACTATGGCCCTTCTGCCAAGCCCAGGTGCTAGGGGAGGAACC CCTTGCTTGAGATGTACAGGATGGACTCCTCACTTCTTCTGGGCTTGTGCCCCGGCCCGT TGAATCTTGTCAGCTGTGGCCCCATCTGTGGCTCCCGTGCAGTGGGCCAATACGAGGCTC AGCTCAGCCTCATTCCGGTGTTCGATGGCCACATCTGCAGCCTGAGCCACATCGCCAACA AGAAGCAAAGCCTTGACCTTCTGCTCGGGACCCACGCGGGAAGCATACTTCTTGGCTTCG TATTTGTTATGTTGTTTCATGCAGATCTCCACAAAAGGCAGGTAGCCAATGGGTGATTTC TTGCTCTTGGAAAACTTCTCTAGCTCTTCCCCATCTTCCAAATCTGCCAGGGCAGTCAGC TTCAGCCACCAGAGCCTCTTGTCAGGGATGCGGAAGTCCCGTGCCAGCTGCTCTGCACGC TTGTTGTGACCGCCAAGAATGAGGGTGGTAACTGTGTCATGTAGAGACAGGTCTATGAAC TGGCCCCCCAGCTCGTCTTCTAAGCGCCGCTGCAACCGTAGGAGCCGCATTTGATCCTCT GTAGCCTTGGCTGCAAACTCATTCTTGGCCTTGTANAAGGCATCGGCCGCTGTTTGCAAA GCTGCTACTTGCCCCTCAATACGCTCTTTTGCAGCATATCTGGCTTCCGATTGTGAAGCC TCGCCAATTCCCTGGGGATCGCAATCCTGATTGTAAAGGTCCTTCACAGCCTCTAACTCC TGAAGCTTACGAACTGTCGGACCAAACTGAGGGCCATGGCCTGATTCGCAGGATCATGAA AAAATCTCCTGGTTCAACTCGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022575 |
| Insert Size | 2840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022575.2, NP_072097.2 |
| RefSeq Size | 2769 bp |
| RefSeq ORF | 2520 bp |
| Locus ID | 64601 |
| UniProt ID | Q9H269 |
| Domains | Vps16_C, Vps16_N |
| Protein Families | Druggable Genome |
| Gene Summary | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216121 | VPS16 (Myc-DDK-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 1 |
CNY 6,144.00 |
|
| RC216121L3 | Lenti ORF clone of Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 1, Myc-DDK-tagged |
CNY 10,740.00 |
|
| RC216121L4 | Lenti ORF clone of Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 1, mGFP tagged |
CNY 10,740.00 |
|
| RG216121 | VPS16 (tGFP-tagged) - Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 1 |
CNY 9,690.00 |
