APPD (PLEKHF1) (NM_024310) Human Untagged Clone

Name: APPD (PLEKHF1) (NM_024310) Human Untagged Clone
SKU: SC112275
Price: 2400.00

CAT＃: SC112275

PLEKHF1 (untagged)-Human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1)

Datasheet

SDS

"NM_024310" in other vectors (6)

CNY 2,400.00

CNY 2,950.00

货期*

现货

规格

Product images

经常一起买 (4)

TA363812

APPD Antibody - C-terminal region

CNY 5,250.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APPD; LAPF; PHAFIN1; ZFYVE15
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC112275 sequence for NM_024310 edited (data generated by NextGen Sequencing) ATGGTGGACCACTTGGCCAACACGGAGATCAACAGCCAGCGCATCGCGGCAGTGGAGAGC TGCTTCGGGGCCTCGGGGCAGCCGCTGGCGCTGCCAGGCCGAGTGCTGCTGGGCGAGGGC GTGCTGACCAAAGAGTGCCGCAAGAAGGCCAAGCCGCGCATCTTCTTCCTCTTTAACGAC ATCCTGGTGTATGGCAGCATCGTGCTCAACAAGCGCAAGTACCGCAGCCAGCACATCATC CCCCTGGAGGAGGTCACACTGGAGCTGTTGCCGGAGACGCTGCAGGCCAAGAACCGCTGG ATGATCAAGACGGCCAAGAAGTCCTTTGTGGTGTCGGCCGCCTCCGCTACGGAGCGCCAG GAATGGATTAGCCACATCGAGGAGTGCGTGCGGCGGCAACTGAGGGCCACGGGCCGCCCG CCCAGCACGGAGCACGCGGCACCCTGGATCCCCGACAAGGCCACGGACATCTGCATGCGC TGCACGCAGACGCGCTTCTCTGCCCTCACGAGGCGCCACCACTGCCGCAAGTGCGGCTTC GTGGTCTGCGCTGAGTGCTCGCGCCAGCGCTTCCTGCTCCCGCGCCTGTCCCCCAAGCCC GTGCGCGTCTGCAGCCTCTGCTACCGCGAACTGGCCGCCCAGCAGCGGCAGGAGGAGGCG GAGGAGCAGGGCGCGGGGTCCCCAGGGCAGCCAGCCCACCTGGCCCGGCCCATCTGCGGA GCGTCCAGTGGAGATGACGATGACTCCGACGAGGACAAGGAGGGCAGCAGGGACGGCGAC TGGCCCAGCAGCGTGGAGTTCTACGCCTCGGGGGTGGCCTGGTCTGCCTTCCACAGCTGA Clone variation with respect to NM_024310.4 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_024310 unedited NGGGTGCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGGACT CGAGGGCTGGGCGCGGGGCCGGCGCAGAAGCCGCCAGCTGGAGACGATGGTGGACCACTT GGCCAACACGGAGATCAACAGCCAGCGCATCGCGGCAGTGGAGAGCTGCTTCGGGGCCTC GGGGCAGCCGCTGGCGCTGCCAGGCCGAGTGCTGCTGGGCGAGGGCGTGCTGACCAAAGA GTGCCGCAAGAAGGCCAAGCCGCGCATCTTCTTCCTCTTTAACGACATCCTGGTGTATGG CAGCATCGTGCTCAACAAGCGCAAGTACCGCAGCCAGCACATCATCCCCCTGGAGGAGGT CACACTGGAGCTGTTGCCGGAGACGCTGCAGGCCAAGAACCGCTGGATGATCAAGACGGC CAAGAAGTCCTTTGTGGTGTCGGCCGCCTCCGCTACGGAGCGCCAGGAATGGATTAGCCA CATCGAGGAGTGCGTGCGGCGGCAACTGAGGGCCACGGGCCGCCCGCCCAGCACGGAGCA CGCGGCACCCTGGATCCCCGACAAGGCCACGGACATCTGCATGCGCTGCACGCAGACGCG CTTCTCTGCCCTCACGAGGCGCCACCACTGCCGCAAGTGCGGCTTCGTGGTCTGCGCTGA GTGCTCGCGCCAGCGCTTCCTGCTCCCGCGCCTGTCCCCCAAGCCCGTGCGCGTCTGCAG CCTCTGCTACCGCGAACTGGCCGCCCANCANCGGCANNGAGAGGGCGGAGANCAGGNCGC GGNGTCCCCAGGGCAGCCAGCCCACCTGGCCCCGGCCCATCTGCNNGAGCGTCCAGTGGA GATGACGATGACTCCGACGAGGGACAGGAGGGCAGCAGGGACGGCGACTGGGCCANCAGC G 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_024310 unedited ATGTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTTCAATAAACTT TCTTTTTTATTATTAAGTGGGGGCAGGGTTTCTGTTCTTGCAACTGAGTCCTAACAAAAA ACAATGGTTTCGCTGACCACACGGAGAGCTGAGGACAGGACAAAAAGGCATGATACAGCT GGACACCTGGAGAGAGGTGACACAGGACAGAGTCCTCACCCGGCTGCCCTGGGCCTCGGG GAGCTCAGGCTGCAGTCCCTGGGCCTGGTGGCACCCACAGCACGGCAGTCTCTGGCTGGG CTTCGGGGAGCCCACATGTCTGATCGGCAGGGCTTGGCTGGCCAGGCGGTGGGCACCAGC GTGGTGGGTGGGCAGTCCTGGGCTTCCAGGAGGCCTCCTGGGACTCAGGCTCTTCAGTGG GGTGTGCCTGTCAGGGTGCAGGTGGGTTCTGTGGCTACACTCCCATCCTGTCTTCCCTCT GTGTTCCCAAACCTGCTCTGGTCCCTATATGTCTGGAGACCTGGGGTCGTCCAGCTCAGG AGGCCATCTGGAGCTGCCGCTGCATCCTGCATAGTGTAAGGTGGNCGTTTGTTACCAAGA CCTGTAGGGTGTTCTGGGGGCTTCCTGAAGGGAAAGGCATTACCTGCAGTGAAGCCATAA GGACACATGTGTCCAGCATAAAGGCACTGGGGAGTCCAGAAAGAGCCACTCCCCACCGCT GCACCTGCCACATGGNAATGGGGGTCCGGNAGCCCTGGGCAGCTTCTGGAGCTGCCTCTT TGGAGCCTGGGCATGGGAACTGGCTGGAAACANATGTCTGAGACCCGCGGGTTAACTTTG GGAAAGCAAAACAGCCACCCCCAGGGGTAAACTCCAGCTGCTGGGCCAATTGCGGCCCTC TGGCCTCTGTCTTTNGGGGAATATGGAATCTCATGGACCTCCAATATGCGGCCCAAATGG GGGGGGTGTCTTGGAAACCCGCCCTTTCTTCCCTC
Restriction Sites	NotI-NotI
ACCN	NM_024310
Insert Size	1850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024310.2, NP_077286.2
RefSeq Size	2094 bp
RefSeq ORF	840 bp
Locus ID	79156
UniProt ID	Q96S99
Domains	FYVE, PH
Gene Summary	May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC201282	PLEKHF1 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,990.00
RC201282L1	Lenti ORF clone of Human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC201282L2	Lenti ORF clone of Human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC201282L3	Lenti ORF clone of Human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC201282L4	Lenti ORF clone of Human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG201282	PLEKHF1 (tGFP-tagged) - Human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,000.00 CNY 4,370.00

其它APPD产品

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Proteins

Antibodies