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ALG12 (NM_024105) Human Untagged Clone
CAT＃: SC112248
ALG12 (untagged)-Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDG1G; ECM39; hALG12; PP14673 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112248 sequence for NM_024105 edited (data generated by NextGen Sequencing)
ATGGCTGGAAAGGGGTCATCAGGCAGGCGGCCCCTGCTGCTGGGGCTGCTGGTGGCCGTA GCCACTGTCCACCTGGTCATCTGTCCCTACACCAAAGTGGAGGAGAGCTTCAACCTGCAG GCCACACATGACCTGCTCTACCACTGGCAAGACCTGGAGCAGTACGACCATCTTGAGTTC CCCGGAGTCGTCCCCAGGACGTTCCTCGGGCCAGTGGTGATCGCAGTGTTCTCCAGCCCC GCGGTTTACGTGCTTTCGCTGTTAGAAATGTCCAAGTTTTACTCTCAGCTAATAGTTAGA GGAGTGCTTGGACTCGGCGTGATTTTTGGACTCTGGACGTTACAAAAGGAAGTGAGACGG CACTTCGGGGCCATGGTGGCCACCATGTTCTGCTGGGTGACGGCCATGCAGTTCCACCTG ATGTTCTACTGCACGCGGACACTGCCCAATGTGCTGGCCCTGCCTGTAGTCCTGCTGGCC CTCGCGGCCTGGCTGCGGCACGAGTGGGCCCGCTTCATCTGGCTGTCAGCCTTCGCCATC ATCGTGTTCAGGGTGGAGCTGTGCCTGTTCCTGGGCCTCCTGCTGCTGCTGGCCTTGGGC AACCGAAAGGTTTCTGTAGTCAGAGCCCTTCGCCACGCCGTCCCGGCAGGGATCCTCTGT TTAGGACTGACGGTTGCTGTGGACTCTTATTTTTGGCGGCAGCTCACTTGGCCGGAAGGA AAGGTGCTTTGGTACAACACTGTCCTGAACAAAAGCTCCAACTGGGGGACCTCCCCGCTG CTGTGGTACTTCTACTCAGCCCTGCCCCGCGGCCTGGGCTGCAGCCTGCTCTTCATCCCC CTGGGCTTGGTAGACAGAAGGACGCACGCGCCGACGGTGCTGGCGCTGGGCTTCATGGCA CTCTACTCCCTCCTGCCACACAAGGAGCTACGCTTCATCATCTATGCCTTCCCCATGCTC AACATCACGGCTGCCAGAGGCTGCTCCTACCTGCTGAATAACTATAAAAAGTCTTGGCTG TACAAAGCGGGGTCTCTGCTTGTGATCGGACACCTCGTGGTGAATGCCGCCTACTCAGCC ACGGCCCTGTATGTGTCCCATTTCAACTACCCAGGTGGCGTCGCAATGCAGAGGCTGCAC CAGCTGGTGCCCCCCCAGACAGACGTCCTTCTGCACGTTGACGTGGCAGCCGCCCAGACA GGTGTGTCTCGGTTTCTCCAAGTCAACAGCGCCTGGAGGTACGACAAGAGGGAGGATGTG CAGCCGGGGACAGGCATGCTGGCATACACACACATCCTCATGGAGGCGGCCCCTGGGCTC CTGGCCCTCTACAGGGACACACACCGGGTCCTGGCCAGCGTCGTGGGGACCACAGGTGTG AGTCTGAACCTGACCCAACTGCCCCCCTTCAACGTCCACCTGCAGACAAAGCTGGTGCTT CTGGAGAGGCTCCCCCGGCCGTCCTGA Clone variation with respect to NM_024105.3 885 a=>g;1177 a=>g >OriGene 5' read for NM_024105 unedited
GCATTTTGTATCCGACTCATATAGGCGGCCGCGCAATTCGCACGAGGCCTCGTGCCGAAT TCGGCACGAGGACAGAGGGTCTCTGCGGCCGTAGCGGCCGGGGCTGCAGTAGCCACTTTA GATTTGGGCAAGGACTTTAGATTCGGGCTCTTCTGTTTCCGCCGTCCCGCTTCCTGCCGA GGCTGGCCCAGGCAGCCGCGCTTCGAAGGACGCCGCCGGGAGCTGCGGAGCATGCGTGGA GTGGCAGTGCTAACGGCTGGTGTCTCGCACTGTTGGCCTGTGAAGGTACGTGAAGCTGAA AGCCTGGAATGGCTGGAAAGGGGTCATCAGGCAGGCGGCCCCTGCTGCTGGGGCTGCTGG TGGCCGTAGCCACTGTCCACCTGGTCATCTGTCCCTACACCAAAGTGGAGGAGAGCTTCA ACCTGCAGGCCACACATGACCTGCTCTACCACTGGCAAGACCTGGAGCAGTACGACCATC TTGAGTTCCCCCGGAGTCGTCCCCAGGACGTTCCTCGGGCCAGTGGCGATCGCAGTGTTC TCCAGCCCCGCGGTTTACGTGCCTTTCGCTGTTAGAAATGTCCAAGTTTTACTCTCAGCT AATAGGTACAGGAGTGCTTGGACTCGGCGTGATTTTTTGGACTCTGGACGCTACACAAGG AAGTGAGACGGCACTTCGGGGCCCTTGGTGGCCACCATGTTTCTGCTGGGTGACTGCCAT GCAGTTCCACCTGATGGTCTACTGCACGCGGCCACTGCCCAATGGGCCTGGCCCTGCCTG TAGTCCTGCTGCCCCTCGCGGCCTGGTTGTGGCAAGAATGGGCCCCGCTTAATCTGGCTG TCAACCCTCCGCCATCATCGGGTCAGGGTGGCACCTGGCCTTGTTCCTGGCCCTCCTGCT GTTGTTGCTCTTGGGCAACCCAAAGGTGTCTGTAGTCAGAACCCTTCTCCACCGCGTCCC GGTAGGGATCCTCT >OriGene 3' read for NM_024105 unedited
ATAGCTATGNAACGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGAGAGG TTCCAATCAACATTTATTGCCTTATTCTTTTTATCTCATTCCTTTTTGAATGTGTTTATC TCCTAAGATTTTATCTGTGATGGAGATGGGATGCCTGTGAATACAAAAGTTGCAGTGGTG GCACCAGGGTGGGGGGGTGCGGCCGGGGCCACCATGGTCTCCCGAGAGGGGGTGCTGTCT TAAGTGCCCCAAGAGGCCCTCGGGCAGCAAGCGTGGGGTGCTGCCAAAATACAGCTCCCC CTGGGTGGGCAGGACACACGTGGCCTCCTGGCAGACAGGTGCCTGGGTGAGCCCGCTGCT CCTGATTAGTCATGAATGGCACCTGGTCTGGGCGACAGTCACCCGCAGGAAGCCCTGAGC TGGCCACCATCACCCTGGGCAGTGGCTCCCGGGGTGCCAACAAGACCTGGGCCCCTCGTT CTTTGGTGCTGAGAGCCCCAGCTGAAGCTGTGGAGGAAGCCCTGGACCTGGTCTGGTGTC TGTCAGAGGCAGGTGCCCAGTCCTTTGACTTGCTTCTCTGAATTGTCATAATTGTGCTGG AATTGTGCCAGAAACTGGTAGCGATAACAGCTCCTGGAAAGCCTGTGGCTGCTGAAGGCT GCCTGGTCCCCCTCAGGACGGCCGGGGGAGCCTCTCCAGAGCACCAGCTTTGTCTGCAGG TGGACGTTGAAGGGGGGCAGTTGGGTCAAGTTCAAACTCACACCTGTGGTCCCCACGACG CTGGCCAGGACCCGGTGTGTGTCCCTGTATAGGCCATGAGCCCAGGGCCGCCTCATGAAG ATTGTGTGTATGCANCATGCTGTNCCCGCTGCCATNCTCCTCTGTCGTACCTCAGCGCTG TGACTGGAAAACCAGAACACTGTCTGGGCGTGCCAGTAACGGCAAAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_024105 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024105.3, NP_077010.1 |
| RefSeq Size | 2387 bp |
| RefSeq ORF | 1467 bp |
| Locus ID | 79087 |
| UniProt ID | Q9BV10 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215501 | ALG12 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12) |
CNY 3,656.00 |
|
| RC215501L3 | Lenti ORF clone of Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215501L4 | Lenti ORF clone of Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12), mGFP tagged |
CNY 5,890.00 |
|
| RG215501 | ALG12 (tGFP-tagged) - Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12) |
CNY 5,256.00 |
