XLF (NHEJ1) (NM_024782) Human Untagged Clone

CAT#: SC112059

NHEJ1 (untagged)-Human nonhomologous end-joining factor 1 (NHEJ1)



  "NM_024782" in other vectors (6)

CNY 6,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
NHEJ1 (XLF) mouse monoclonal antibody, clone OTI3D6 (formerly 3D6)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms XLF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_024782, the custom clone sequence may differ by one or more nucleotides


ATGGAAGAACTGGAGCAAGGCCTGTTGATGCAGCCATGGGCGTGGCTACAGCTTGCAGAGAACTCCCTCT
TGGCCAAGGTTTTTATCACCAAGCAGGGCTATGCCTTGTTGGTTTCAGATCTTCAACAGGTGTGGCATGA
ACAGGTGGACACTAGTGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGGCTCACTGCTCCTCCTGCA
GCTTTCCTCTGTCATTTGGATAATCTCCTTCGCCCATTGTTGAAGGACGCTGCTCACCCTAGCGAAGCTA
CCTTCTCCTGTGATTGTGTGGCAGATGCACTGATTCTACGGGTGCGAAGTGAGCTCTCTGGCCTCCCCTT
CTATTGGAATTTCCACTGCATGCTAGCTAGTCCTTCCCTGGTCTCCCAACATTTGATTCGTCCTCTGATG
GGCATGAGTCTGGCATTACAGTGCCAAGTGAGGGAGCTAGCAACGTTACTTCATATGAAAGACCTAGAGA
TCCAAGACTACCAGGAGAGTGGGGCTACGCTGATTCGAGATCGATTGAAGACAGAACCATTTGAAGAAAA
TTCCTTCTTGGAACAATTTATGATAGAGAAACTGCCAGAGGCATGCAGCATTGGTGATGGAAAGCCCTTT
GTCATGAATCTGCAGGATCTGTATATGGCAGTCACCACACAAGAGGTCCAAGTGGGACAGAAGCATCAAG
GCGCTGGAGATCCTCATACCTCAAACAGTGCTTCCCTGCAAGGAATCGATAGCCAATGTGTAAACCAGCC
AGAACAACTGGTCTCCTCAGCCCCAACCCTCTCAGCACCTGAGAAAGAGTCCACGGGTACTTCAGGCCCT
CTGCAGAGACCTCAGCTGTCAAAGGTCAAGAGGAAGAAGCCAAGGGGTCTCTTCAGTTAA


>OriGene 5' read for NM_024782 unedited
NGGTGCGGAATAGTATACGATTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGATGAT
GGAAGAACTGGAGCAAGGCCTGTTGATGCAGCCATGGGCGTGGCTACAGCTTGCAGAGAA
CTCCCTCTTGGCCAAGGTTTTTATCACCAAGCAGGGCTATGCCTTGTTGGTTTCAGATCT
TCAACAGGTGTGGCATGAACAGGTGGACACTAGTGTGGTCAGCCAGCGAGCCAAGGAGCT
GAACAAGCGGCTCACTGCTCCTCCTGCAGCTTTCCTCTGTCATTTGGATAATCTCCTTCG
CCCATTGCTGAAGGACGCTGCTCACCCTAGCGAAGCTACCTTCTCCTGTGATTGTGTGGC
AGATGCACTGATTCTACGGGTGCGAAGTGAGCTCTCTGGCCTCCCCTTCTATTGGAATTT
CCACTGCATGCTAGCTAGTCCTTCCCTGGTCTCCCAACATTTGATTCGTCCTCTGATGGG
CATGAGTCTGGCATTACAGTGCCAAGTGAGGGAGCTAGCAACGTTACCTTATATGAAAGA
CCTAGAGATCCAAGACTACCAGGAGAGTGGCGCTACGCTGATTCGCGATCGATTGCAGAC
AGAACCCTTTGAAGAAAATTCCTTCTTGGAACCAATTATGATACAAGAACCTGCCCGAGG
CCATGCAGCATTTGTTGATGGAAAGCCCCTTTGTCATGAATCTGCAGGATCCTGCCTACG
GCAGTCACCCCCACAAGAGGCCCAAGTGGGACAGAAGCCTCAAGGCGGCTGGAGACCCCA
TACCTCAACCAGGCTTTCCCTGCCGGATCCCTCGCCACTGGGAAACCAGCCGAAACACTG
TCTCCTTATTCCACCCTTTAATACCTGATAAAGAGCCCCGGTACTTCAGCCTTCTGCCAA
ACCCCACTGCCAAGGCCAGAGG
Restriction Sites NotI-NotI     
ACCN NM_024782
Insert Size 2770 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024782.1, NP_079058.1
RefSeq Size 2037 bp
RefSeq ORF 900 bp
Locus ID 79840
UniProt ID Q9H9Q4
Protein Pathways Non-homologous end-joining
Gene Summary Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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