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DENND1A (NM_024820) Human Untagged Clone
CAT＃: SC111995
DENND1A (untagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2
CNY 4,168.00
CNY 7,220.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FAM31A; KIAA1608 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_024820 edited
ATGGGCTCCAGGATCAAGCAGAATCCAGAGACCACATTTGAAGTATATGTTGAAGTGGCC TATCCCAGGACAGGTGGCACTCTTTCAGATCCTGAGGTGCAGAGGCAATTCCCGGAGGAC TACAGTGACCAGGAAGTTCTACAGACTTTGACCAAGTTTTGTTTCCCCTTCTATGTGGAC AGCCTCACAGTTAGCCAAGTTGGCCAGAACTTCACATTCGTGCTCACTGACATTGACAGC AAACAGAGATTCGGGTTCTGCCGCTTATCTTCAGGAGCGAAGAGCTGCTTCTGTATCTTA AGCT:ATCTCCCCTGGTTCGAGGTATTTTATAAGCTGCTTAACATCCTGGCAGATTACAC GACAAAAAGACAGGAAAATCAGTGGAATGAGCTTCTTGAAACTCTGCACAAACTTCCCAT CCCTGACCCAGGAGTGTCTGTCCATCTCAGCGTGCATTCTTATTTTACTGTGCCTGATAC CAGAGAACTTCCCAGCATACCTGAGAATAGAAATCTGACAGAATATTTTGTGGCTGTGGA TGTTAACAACATGTTGCATCTGTACGCCAGTATGCTGTACGAACGCCGGATACTCATCAT TTGCAGCAAACTCAGCACTCTGACTGCCTGCATCCACGGGTCTGCGGCGATGCTCTACCC CATGTACTGGCAGCACGTGTACATCCCCGTGCTGCCGCCGCATCTGCTGGACTACTGCTG TGCTCCCATGCCCTACCTCATAGGAATCCATTTAAGTTTAATGGAGAAAGTCAGAAACAT GGCCCTGGATGATGTCGTGATCCTGAATGTGGACACCAACACCCTGGAAACCCCCTTCGA TGACCTCCAGAGCCTCCCAAACGACGTGATCTCTTCCCTGAAGAACAGGCTGAAAAAGGT CTCCACAACCACTGGGGATGGTGTGGCCAGAGCGTTCCTCAAGGCCCAGGCTGCTTTCTT CGGTAGCTACCGAAACGCTCTGAAAATCGAGCCGGAGGAGCCGATCACTTTCTGTGAGGA AGCCTTCGTGTCCCACTACCGCTCCGGAGCCATGAGGCAGTTCCTGCAGAACGCCACACA GCTGCAGCTCTTCAAGCAGTTTATTGATGGTCGATTAGATCTTCTCAATTCCGGCGAAGG TTTCAGTGATGTTTTTGAAGAGGAAATCAACATGGGCGAGTACGCTGGCAGTGACAAACT GTACCATCAGTGGCTCTCCACTGTCCGGAAAGGAAGTGGAGCAATTCTGAATACTGTAAA GACCAAAGCAAATCCGGCCATGAAGACTGTCTACAAGTTCGCAAAAGATCATGCAAAAAT GGGAATAAAAGAGGTGAAAAACCGCTTGAAGCAAAAGGACATTGCCGAGAATGGCTGCGC CCCCACCCCAGAAGAGCAGCTGCCAAAGACTGCACCGTCCCCACTGGTGGAGGCCAAGGA CCCCAAGCTCCGAGAAGACCGGCGGCCAATCACAGTCCACTTTGGACAGGTGCGCCCACC TCGTCCACATGTTGTTAAGAGACCAAAGAGCAACATCGCAGTGGAAGGCCGGAGGACGTC TGTGCCGAGCCCTGAGCAAAACACCATTGCAACACCAGCTACACTCCACATCCTACAGAA AAGCATTACCCATTTTGCGGCCAAGTTCCCGACGAGAGGCTGGACCTCTTCATCACATTG A |
| Restriction Sites | NotI-NotI |
| ACCN | NM_024820 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_024820.2. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024820.2, NP_079096.2 |
| RefSeq Size | 2166 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 57706 |
| UniProt ID | Q8TEH3 |
| Domains | DENN, dDENN, uDENN |
| Gene Summary | Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010] Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1, that causes a frameshift. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Human DENND1A.V2 Drives Cyp17a1 Expression and Androgen Production in Mouse Ovaries and Adrenals
,Teves, ME;Modi, BP;Kulkarni, R;Han, AX;Marks, JS;Subler, MA;Windle, J;Newall, JM;McAllister, JM;Strauss, JF;,
Int J Mol Sci
,PubMed ID 32268539
[DENND1A]
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Co-Localization of Polycystic Ovary Syndrome Candidate Gene Products in Theca Cells Suggests Novel Signaling Pathways
,Kulkarni, R;Teves, M;Han, A;McAllister, J;Strauss, J;,
Journal of the Endocrine Society
[DENND1A]
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Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype
,McAllister, JM;Modi, B;Miller, BA;Biegler, J;Bruggeman, R;Legro, RS;Strauss, JF;,
Proc. Natl. Acad. Sci. U.S.A. E1519-27 111 15. April 2014
,PubMed ID 24706793
[DENND1A]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207881 | DENND1A (Myc-DDK-tagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2 |
CNY 4,160.00 |
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| RC207881L1 | Lenti ORF clone of Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2, Myc-DDK-tagged |
CNY 6,560.00 |
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| RC207881L2 | Lenti ORF clone of Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC207881L3 | Lenti ORF clone of Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207881L4 | Lenti ORF clone of Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
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| RG207881 | DENND1A (tGFP-tagged) - Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2 |
CNY 5,760.00 |
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