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MBD1 (NM_002384) Human Untagged Clone
CAT＃: SC111970
MBD1 (untagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 4
CNY 3,752.00
CNY 8,080.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CXXC3; PCM1; RFT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002384, the custom clone sequence may differ by one or more nucleotides
ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAGT CAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAGT TGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTGTGC TATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCAGCCA AGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAGACCAA GGCTGACACTGACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTC TCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGAATTGCCT TCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTAACAGAAGA CTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGTTCTGCAAGTGT GAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAGTATGCCGGGGCTGTCAGACCC AAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGCCAGTGGAAATG TGTCCAGCGACGTTGCCTACGGGGTAAACATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGGCTGCC AGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCCACAGAGCCGC ACCCCAGAGCCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGTGTAGACGAGGACGAGCTAAA GCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCGCCCCCACCTTACCGTCGTCGA AAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACCTTGAAGCCCACCTTGGCTACACGCACAG CCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAGCAGGTGGTGGCTTTGTGCTGCCCCCGCCTGG CACTGACCTTGTGTTTTTACGGGAAGGCGCAAGCAGTCCTGTGCAGGTGCCGGGCCCTGTTGCAGCTTCC ACAGAAGCCCTGTTGCAGGCAGTAGACCCAGGCCTGCCTTCTGTGAAGCAAGAGCCACCTGACCCAGAGG AGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCCCCAGAGGAAGAGGCAGGAGGGGCTGG CACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCTTCCGAGATACAGCAGTCTGGTTGCCA AGGTCCAAAGACCTTAAAAAACCTGGAGCTAGAAAGCAGTAG >OriGene 5' read for NM_002384 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTACCGCTTCAGAGG AGGCGGCCGCGGAGGAGGAGGAAGGGGAGGAGGGCGAGGCGGGAGGTGCAGGAGGGACCC TCGCCATGGGTCCACGGGCCTAGAGTGGCGGAAGATACCGGCCTGGTGCCAAACTGGCTA CTGCTGCTTCCTGTGGCCTCCATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCT GGCTGGAAGCGCCGCGAAGTCTTTCGCAAGTCAGGGGCCACCTGTGGACGCTCAGACACC TATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAGTTGAGCTGACTCGATACCTG NGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTGTGCTATCCAGCC CCCAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCAGCCA AGACTTCGAAACGTCAGGTTGGACCCCACAGTGGTGAGGTCANGAAAGAGGCCCCCGAGG ATGAGACCAAGGCTGACACTGACACAGCCCCAGCTTCATTTCCTGCTCCTGGGTGCTGTG AGAACTGTGGAATCACCTTCTCAGGGGATGGCACCCCAAAGCAGCGGCTCAAAACGTTGT GCAAAGACTGTTCAGCACAGAGAATTGCCTTCACCGGGAACAGAGAATGTTAAACCCTGT GGCCTGTGGGGAGTGTGCACCCTGCCAGTAACAAAAGACTGGGGGGCCTGTTTCACTTGC CTCCTTGANTTTGCCCATGAGGTGGGATCGGGGCTGTTTTTCCAGTGGAAACGGAAACCC TCCCTCCCCATTGGGGAAAGAACCCCGGTTGTGGAAAATTCCCGGGCCTTCACAACCAAA AGGAATGTGCCCATTCCCCCCCN >OriGene 3' read for NM_002384 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAATACATATTGATGCATTTAA TAAAATTGCATGGGGCCGTATCTTTTGCATTTCTGTATCCTAGTATTAAGTACAGTGCCT ACCACAGGCCAGGTTCTCAATACTTGAATAAATGACACAAAAATAGCCAGGACCAGCAAG TTTTTTCTGGGTGGGCTTCATAGAATCTCTGTACTTGCTGGAATCACCATGAAATCCATG GTCTTCAGCTTTGCATTTTCAGCAACATAGTTATATTGAGTTATCCTCAGGTGAGCAGTG AGACCCTTGGGAGCGGATTCATGGTAGGGTGGCTTTGTAATGTGTCACCTTGGTGAGGCT ATGTTTCCCGGAACTCTCTTCCAGTAAGATGGGCCACAAGAGACATTTTGCTTGATAACC GGAGGGCGGAAGTGAAGCAGCAGCACATTGTTTTTACACTTGGAAGGTTGGTGCTGGGGC ACCAGGCGTTGTTGCAGTTCACACGTTGCCATGTATCTGCTAGATCACCTCGTTGGTGTG GGCAGTAGTCAGGCCTGCAGCCAGGCCTGCAGCTGCTCCACCTTCCCCAGGATCATCCTT TCAGCTTCTCTAATTCCTGNGCCAGATGCATATTTAACTCTGTGAGGAAGGGCACCCAGC TTCTTCTGCAGGACACCCACATCATCGAAGCTTGAGGTGGTGAGAGACTGACATGGGTTC CAGGCCATCCTNGTGGGTTCCAACTCGTGCTCGTGGGCTTCACTGGGTCCTCGGCCTCCA CACTTTACATACCTCTTCCCTTCCCGAAGCCTGCCCTGGAGACTTGATCCCACATCCGGA GAAACCTCCACCTACGCCTTTTACCCCAAGTTTTTAAGGGCTTGGACCTGGAACCAACCG CTGTATCTGGACCGGTTCAACCCGCCTGAAACTCCGGACACGGGGGGCAACCTCCTGCTC TCTTGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002384 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002384.1, NP_002375.1 |
| RefSeq Size | 2978 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 4152 |
| UniProt ID | Q9UIS9 |
| Domains | MBD, zf-CXXC |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (4) lacks 2 in-frame non-consecutive coding exons compared to variant 1, resulting in a shorter isoform (4) missing two internal protein segments compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224224 | MBD1 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 4 |
CNY 4,560.00 |
|
| RC224224L3 | Lenti-ORF clone of MBD1 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 4 |
CNY 6,460.00 |
|
| RC224224L4 | Lenti-ORF clone of MBD1 (mGFP-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 4 |
CNY 6,460.00 |
|
| RG224224 | MBD1 (tGFP-tagged) - Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 4 |
CNY 5,040.00 |
