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GLS2 (NM_013267) Human Untagged Clone
CAT＃: SC111793
GLS2 (untagged)-Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein
CNY 6,736.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GA; GLS; hLGA; LGA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111793 sequence for NM_013267 edited (data generated by NextGen Sequencing)
ATGCGCTCCATGAAGGCTCTGCAGAAGGCCCTGAGCCGGGCTGGCAGTCACTGCGGGCGA GGAGGCTGGGGTCACCCGAGCCGGAGCCCCCTCCTTGGCGGGGGCGTCCGGCACCACCTC AGTGAGGCCGCGGCGCAGGGCAGAGAGACGCCACACAGCCACCAGCCGCAGCACCAGGAT CATGATTCATCAGAAAGTGGCATGCTGTCCCGCCTGGGTGATTTGCTCTTTTACACTATT GCTGAAGGACAGGAACGAATCCCTATCCACAAGTTCACCACTGCACTAAAGGCCACTGGA CTGCAGACATCAGATCCTCGGCTCCGAGACTGCATGAGCGAGATGCACCACGTGGTCCAA GAGTCCAGTAGTGGTGGCCTCTTGGACCGAGATCTCTTCCGAAAGTGTGTGAGCAGCAAC ATTGTGCTCCTGACCCAGGCATTCCGAAAGAAGTTTGTCATTCCTGATTTTGAGGAGTTC ACGGGCCATGTGGATCGCATCTTTGAGGATGTCAAAGAGCTCACTGGAGGCAAAGTGGCA GCCTACATCCCTCAGCTGGCCAAGTCAAACCCAGACCTGTGGGGTGTCTCCCTGTGCACT GTGGATGGTCAACGGCACTCTGTGGGCCACACAAAGATCCCCTTCTGCCTGCAGTCCTGT GTGAAGCCCCTCACCTATGCCATCTCCATAAGCACCCTAGGCACTGACTACGTGCACAAG TTTGTGGGCAAAGAGCCAAGTGGCCTGCGCTACAACAAGCTCTCCCTCAATGAGGAAGGA ATCCCCCATAACCCCATGGTCAATGCTGGTGCCATTGTTGTCAGCTCCCTGATCAAGATG GACTGTAACAAAGCAGAGAAGTTTGATTTTGTGTTGCAGTATCTCAACAAAATGGCTGGG AATGAATACATGGGTTTCAGCAATGCCACATTCCAGTCAGAGAAGGAAACAGGGGATCGG AATTATGCCATCGGCTATTATCTCAAGGAAAAGAAGTGCTTTCCTAAGGGGGTGGACATG ATGGCTGCCCTTGATCTCTACTTCCAGCTGTGTTCTGTGGAGGTCACTTGTGAATCAGGC AGTGTCATGGCAGCCACCCTCGCCAACGGTGGGATCTGCCCCATCACAGGCGAGAGTGTG CTGAGTGCTGAAGCAGTGCGCAACACCCTCAGCCTCATGCATTCCTGCGGCATGTATGAC TTCTCTGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTATCAGGAGCC ATCCTCCTGGTGGTACCCAATGTCATGGGAATGATGTGCCTGTCACCCCCATTGGACAAG CTGGGGAACAGCCATAGGGGGACCAGCTTCTGCCAGAAGTTGGTGTCTCTCTTCAATTTC CACAACTATGACAACCTGAGGCACTGTGCTCGGAAGTTAGACCCACGGCGTGAAGGGGCA GAAATTCGGAACAAGACTGTGGTCAACCTGTTATTTGCTGCCTATAGTGGCGATGTCTCA GCTCTTCGAAGGTTTGCCTTGTCAGCCATGGATATGGAACAGAAAGACTATGACTCGCGC ACAGCTCTGCATGTTGCTGCAGCTGAAGGACACATCGAAGTTGTTAAATTCCTGATCGAG GCTTGCAAAGTGAATCCTTTTGCCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGCT GTGCAGTTCAACCATCTGGAGGTGGTCAAACTGCTTCAAGATTACCAGGACTCCTACACA CTCTCTGAAACTCAGGCTGAGGCAGCAGCTGAGGCCCTGTCCAAAGAGAACTTAGAAAGC ATGGTATGA Clone variation with respect to NM_013267.2 350 g=>a >OriGene 5' read for NM_013267 unedited
CCCCCGTTCAAAATTGTAAACGACTCACTATAGGCGGCCTGCGAATCGGCACGAGGCTTG CTGGGGCATGCGCTCCATGAAGGCTCTGCAGAAGGCCCTGAGCCGGGCTGGCAGTCACTG CGGGCGAGGAGGCTGGGGTCACCCGAGCCGGAGCCCCCTCCTTGGCGGGGGCGTCCGGCA CCACCTCAGTGAGGCCGCGGCGCAGGGCAGAGAGACGCCACACAGCCACCAGCCGCAGCA CCAGGATCATGATTCATCAGAAAGTGGCATGCTGTCCCGCCTGGGTGATTTGCTCTTTTA CACTATTGCTGAAGGACAGGAACGAATCCCTATCCACAAGTTCACCACTGCACTAAAGGC CACTGGACTGCAGACATCAGATCCTCGGCTCCGAGACTGCATGAGCGAGATGCACCACGT GGTCCAAGAGTCCAGTAGTGGTGGCCTCTTGGACCGAGATCTCTTCCGAAAGTGTGTGAG CAGCAACATTGTGCTCCTGACCCAGGCATTCCGAAAGAAGTTTGTCATTCCTGATTTTGA GGAGTTCACGGGCCATGTGGATCGCATCTTTGAGGATGTCAAAGAGCTCACTGGAGGCAA AGTGGCAGCCTACATCCCTCAGCTGGCCAAGTCAAACCCAGACCTGTGGGGTGTCTCCCT GTGCACTGTGGATGGTCAACGGCACTCTGTGGGCCACACAAAGATCCCCTTCTGCCTGCA GTCCTGTGTGAAGCCCCTCACCTATGCCATCTNCATAAGCACCCTAGGCACTGACTACGT GCACAAGTTTGTGGGCACAGAGCCAAGTGGCCTGCGCTACAAACACGCTCTCCTCATGAG GCAGGAATTCCCCATAACCCATGGTCAAGCTGGTGCCATTGTTGTCAGCTCCTGATCAGA TGGACTG >OriGene 3' read for NM_013267 unedited
TTTTTTTTTTTTTTTTTGATTTGGTAATTAAAGTTTATTTGAACACAAAATACTTNTCTC TGTCTATAAAATTGGCTGTTAGCAGTAGCAGCAGCATGTCCTGGCCAAGGGGAGTAGATT TCTCCAGACTACTAAAGCCATGTATATAGCCATTCCCACTTCCCATATTCTGTGGATATG TACATGTGCATGGTAGCTAGAGTCCCTCCACCAGAGTATCTCTCTCATGATGGTTTCTTC AGGGAGAGGAAGAGGAGACCTGGGTGAAGAGCTGGGATGGTTTTGAGTGGGGCAAGCCAT TAGGCTGTACCTTGAAGN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013267 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013267.2, NP_037399.2 |
| RefSeq Size | 2636 bp |
| RefSeq ORF | 1809 bp |
| Locus ID | 27165 |
| UniProt ID | Q9UI32 |
| Domains | ANK, Glutaminase |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism |
| Gene Summary | The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212650 | GLS2 (Myc-DDK-tagged)-Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein |
CNY 6,720.00 |
|
| RC212650L1 | Lenti ORF clone of Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 9,120.00 |
|
| RC212650L2 | Lenti ORF clone of Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 7,130.00 |
|
| RC212650L3 | Lenti ORF clone of Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,130.00 |
|
| RC212650L4 | Lenti ORF clone of Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 7,130.00 |
|
| RG212650 | GLS2 (tGFP-tagged) - Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein |
CNY 8,320.00 |
