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PDGF beta (PDGFB) (NM_002608) Human Untagged Clone
CAT＃: SC111665
PDGFB (untagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1
CNY 3,600.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111665 sequence for NM_002608 edited (data generated by NextGen Sequencing)
ATGAATCGCTGCTGGGCGCTCTTCCTGTCTCTCTGCTGCTACCTGCGTCTGGTCAGCGCC GAGGGGGACCCCATTCCCGAGGAGCTTTATGAGATGCTGAGTGACCACTCGATCCGCTCC TTTGATGATCTCCAACGCCTGCTGCACGGAGACCCCGGAGAGGAAGATGGGGCCGAGTTG GACCTGAACATGACCCGCTCCCACTCTGGAGGCGAGCTGGAGAGCTTGGCTCGTGGAAGA AGGAGCCTGGGTTCCCTGACCATTGCTGAGCCGGCCATGATCGCCGAGTGCAAGACGCGC ACCGAGGTGTTCGAGATCTCCCGGCGCCTCATAGACCGCACCAACGCCAACTTCCTGGTG TGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAACAACCGCAACGTGCAG TGCCGCCCCACCCAGGTGCAGCTGCGACCTGTCCAGGTGAGAAAGATCGAGATTGTGCGG AAGAAGCCAATCTTTAAGAAGGCCACGGTGACGCTGGAAGACCACCTGGCATGCAAGTGT GAGACAGTGGCAGCTGCACGGCCTGTGACCCGAAGCCCGGGGGGTTCCCAGGAGCAGCGA GCCAAAACGCCCCAAACTCGGGTGACCATTCGGACGGTGCGAGTCCGCCGGCCCCCCAAG GGCAAGCACCGGAAATTCAAGCACACGCATGACAAGACGGCACTGAAGGAGACCCTTGGA GCCTAG Clone variation with respect to NM_002608.2 >OriGene 5' read for NM_002608 unedited
NGGGTAATACCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG GCCGCGAATTCGGCACGAGGGCGGACCCGAGCCGAGCCCACCCCCCTCCCCAGCCCCCCA CCCTGGCCGCGGGGGCGGCGCGCTCGATCTACGCGTCCGGGGCCCCGCGGGGCCGGGCCC GGAGTCGGCATGAATCGCTGCTGGGCGCTCTTCCTGTCTCTCTGCTGCTACCTGCGTCTG GTCAGCGCCGAGGGGGACCCCATTCCCGAGGAGCTTTATGAGATGCTGAGTGACCACTCG ATCCGCTCCTTTGATGATCTCCAACGCCTGCTGCACGGAGACCCCGGAGAGGAAGATGGG GCCGAGTTGGACCTGAACATGACCCGCTCCCACTCTGGAGGCGAGCTGGAGAGCTTGGCT CGTGGAAGAAGGAGCCTGGGTTCCCTGACCATTGCTGAGCCGGCCATGATCGCCGAGTGC AAGACGCGCACCGAGGTGTTCGAGATCTCCCGGCGCCTCATAGACCGCACCAACGCCAAC TTCCTGGTGTGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAACAACCGC AACGTGCAGTGCCGCCCCACCCAGGTGCAGCTGCGACCTGTCCAGGTGAGAAAGATCGAG ATTGTGCGGAAGAAGCCAATCTTTAAGAAGGCCACGGTGACGCTGGAAGACCACCTGGNC ATGCAGTGTGAGACAGTGGCAGCTGCACGGCCTGTGACCCGAGCCCGGNGGGGTCCCAGG ACAGCGAGCCAAACGCCCCCAACTCGGTGACCATTCGACGGTGCGAGTCCGCCGGCCC >OriGene 3' read for NM_002608 unedited
NGGGAATACTATGTACCCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTCCGGCGGATTCATTTGGTTTTGTTTTGGGGTTATTTTTTTTT ACAACTTTAAATACGGAATATAAATAAATTTTACATTTAAAAAATAAAAGGAAAGCCCCC AAAAATATAATCACCGACTTTACAAACTGAAGGAAGCAGGTTTTTTAAAGCGGGAAGGGG GAAAGTGCAGTAGGTGGGAAGGGAGGTGGGAGCTCAAATCCCACCCCAGGGGGTCTTGGG GATCTTTTCCCTTTTTTCCCCCATTCCCAGCCCACGAGCTGGTTTCCTAGGAGGAGCCAC CAGAGGTGGAGCTCAAAAGGATCAAAGATGGGGAAGTGTGCAAGCAGGGAGGGGTGCGGG CAAAAAGGCTGCACCCTCCCTTGGAGACCTTCTCCCAGGTGTCCCACACCCACCTGGAAG GGCAGTTGCTGGAGCAAAGGACTTTGGGAAATGGAGGTCATGTGGACAGCAGTGTTGCCT CCCTGCCCCTACCACAGTCTCCCTCCTATTTGGCCACTCCAAGCCACAGGCTGGATCAGG GGCACAGCCCGTCCACAAGGAGTCTTTGTGTGAATTAAGAAAAGGATCCTTTTCCTCTTT ACCTACATCTGCCAGAAAATGGCATAATAAATATACAAATCAGCTTTATTTATGATGTGG ACAGTGCTCCCTGGATACAAAGTGCTTGGGGAGTACACACCCTGGACAAACTTACATGGG AATCGTGGGTGGGTTGGAATATAAAGAGGAAGTCCCTTGCTGGCCTGGCCTCTTATTTTT TGCCCTAAAAAGGAAGGCTGGGATCCATTGGGGGCCTTAAAAAGGGGTCAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002608 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002608.1, NP_002599.1 |
| RefSeq Size | 3373 bp |
| RefSeq ORF | 726 bp |
| Locus ID | 5155 |
| UniProt ID | P01127 |
| Domains | PDGF, PDGF_N |
| Protein Families | Druggable Genome |
| Protein Pathways | Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma |
| Gene Summary | This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1), which contains a signal peptide. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor
,Elangovan, S;D'Mello, SR;Hong, L;Ross, RD;Allamargot, C;Dawson, DV;Stanford, CM;Johnson, GK;Sumner, DR;Salem, AK;,
Mol. Cell. Biol., Aug 2013; 33: 3214 - 3226.
,PubMed ID 24161167
[PDGFB]
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Defective N-sulfation of heparan sulfate proteoglycans limits PDGF-BB binding and pericyte recruitment in vascular development
,A Abramsson, S Kurup, M Busse, S Yamada, P Lindblom, E Schallmeiner, D Stenzel, D Sauvaget, J Ledin, M Ringvall, U Landegren, L Kjellen, G Bondjers, JP Li, U Lindahl, D Spillmann, C Betsholtz, and H Gerhardt,
Genes Dev. 2007 Feb 1;21(3):316-31.
[PDGFB]
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Other Versions
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| RC206587L2 | Lenti ORF clone of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, mGFP tagged |
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| RC206587L3 | Lenti ORF clone of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, Myc-DDK-tagged |
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| RC206587L4 | Lenti ORF clone of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, mGFP tagged |
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| RG206587 | PDGFB (tGFP-tagged) - Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1 |
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| SC324287 | PDGFB (untagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1 |
CNY 3,600.00 |
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