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DNMT3B (NM_006892) Human Untagged Clone
CAT＃: SC111622
DNMT3B (untagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 1
CNY 9,384.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ICF; ICF1; M.HsaIIIB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_006892 edited
ATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCG ATCCTCGTCAACGGGGCCTGCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCT ATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTG TCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGAT GGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGC CCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCC CCACGTTCCACCCGAGGCCGGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTC CCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCC CCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACG CCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATG GAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGG AAGGAGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCC GCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTGG GTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTG GGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAA GCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGC CCTGGAGACTCATTGGAGGACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTC AAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCGA AGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAG ACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAACAAATG GCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGG AAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGAT CGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTG TGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGT GTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAG CCCTGGAGCTGCTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAG GACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGAATACGAAGCC CCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTG TTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAG TACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGG AATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGC CCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCC AGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAAT TACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTA GCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATG ATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCC GGGATGAACAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGT GGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTC GCCCCTCTGAAGGACTACTTTGCATGTGAATAG >OriGene 5' read for NM_006892 unedited
GCATATGTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCGACCCGCGGC TCCGCCGCCCAGCCGCGCCCCAGCCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCA GGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGG CCTGCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGA TCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTGTCCAGTCTGCTAAGCT ACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAG TCATGCCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTC GAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAG GCCGGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCC TGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCTCTTACC TTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTA CCCCCTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGG AGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAG GGGACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCATGGTGGTGTCTTG GAAGGCCACCTNCAGCGACAGGCTATGTCTGCATGNCGTGGTTCAGTGGTTTGGCGATGG GCAGTCTNCGAGGTCTCTGAGACAACTGGTGACTTGGGGCTGTTCACCAGCACTTTATTN GGCCACTTTCATAGCT >OriGene 3' read for NM_006892 unedited
ACGACTNTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAGACAA ATACTGATTTTAATTAAACATAAGGTAAACTCTAGGCATCCGTCATCTTTCAGCCTAAAA ATTAGCAAAAACTGTTGAAACAAGGCACAGTTTTTTCCCCATATTTGTTACGTCGTGGCT CCAGTTACAAAAAAATTTTAATGAAAACGTTAAACATAAAAATAGAAGTTTGAGATTTTA AAAAGTGTATAAAAAGCCCCACAAAACTTGTCAACGTTGTTCCTTATTCTACAAAATAGC ACCAGTAAGAAGAGTAAAAGGTGTTAAAAACCATTATGACAGCATTTCTGAAATGCAGCT TGTCTGAATTCCCGTTCTCCCTAAAAACGACTTCTTATGGAATAAAAAAGGATTAAAAAA TCTCCAAAGGGAGCACCGAGCTTTGCAGTTTTCCCTGTCATCTCTCAGATGTGGGGAAGG TATGAGAAATGTATGTCTGTCCCTGACTGCTGTCACTGCCTCTGAGTTTAGTAAAAAGAT GAGAAATGAGGGTAGCAGACTTCTCATCTGGGGACCTGTGCCTGTGGAGGGTAGGTCTCC TGGAGAGGGAATGGGTTTGAGGGGGTGTCTTATCTAAGCTCCTTGCTTCACACTCCTCTC CTAGGGAAGAGGGAGATACGGAGGAGCCATACTTGTCTACAAAATATATGGCAGCTTATA TTATAGAACTCAGCACACCCCTTCCTGAGCTCCCCAGAGGGTTCTATTGCAGTCACGGGG AGGGATTTAGCTAACAGTGGCCCCTGCCTTAGGGTTTGTGGGGGAAAAAATAGCCCTGAT GTGATGATGACGTCATCCCTGCTGAAATATCATCATTGTGGAAATACTCTATAAGTCCCG TGCAGACTCCAGCCCTGNATTTATGCANGNGCAATGTGCCTGGTAAAGACAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006892 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006892.3, NP_008823.1 |
| RefSeq Size | 4353 bp |
| RefSeq ORF | 2562 bp |
| Locus ID | 1789 |
| UniProt ID | Q9UBC3 |
| Domains | PWWP, DNA_methylase |
| Protein Families | Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| Gene Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PML-RARA requires DNA methytransferase 3A to initiate acute promyelocytic leukemia
,Cole, CB;Verdoni, AM;Ketkar, S;Leight, ER;Russler-Germain, DA;Lamprecht, TL;Demeter, RT;Magrini, V;Ley, TJ;,
J. Clin. Invest.
,PubMed ID 26595813
[DNMT3B]
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| RC223206L2 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 1, mGFP tagged |
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| RC223206L4 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 1, mGFP tagged |
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| RG223206 | DNMT3B (tGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 1 |
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