United States
Germany
Japan
United Kingdom
China
DGKB (NM_004080) Human Untagged Clone
CAT＃: SC111619
DGKB (untagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 1
CNY 5,896.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAGK2; DGK; DGK-BETA |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004080, the custom clone sequence may differ by one or more nucleotides
ATGACAAACCAGGAAAAATGGGCCCACCTCAGCCCTTCGGAATTTTCCCAACTTCAGAAATATGCTGAGT ATTCTACAAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAATCC TGAAGGGAAACAAGACATTCTTAACCAAACAATAGATTTTGAAGGTTTCAAACTATTCATGAAGACATTC CTGGAAGCCGAGCTTCCTGATGATTTCACTGCACACCTTTTCATGTCATTTAGCAACAAGTTTCCTCATT CTAGTCCAATGGTAAAAAGTAAGCCTGCTCTCCTATCAGGCGGTCTGAGAATGAATAAAGGTGCCATCAC CCCTCCCCGGACTACTTCTCCTGCAAATACGTGTTCCCCAGAAGTAATCCATCTGAAGGACATTGTCTGT TACCTGTCTCTGCTTGAAAGAGGAAGACCTGAGGATAAGCTTGAGTTTATGTTTCGCCTTTATGACACGG ATGGGAATGGCTTCCTGGACAGCTCGGAGCTAGAAAATATCATCAGTCAGATGATGCATGTTGCAGAATA CCTTGAGTGGGATGTCACTGAACTTAATCCAATCCTCCATGAAATGATGGAAGAAATTGACTATGATCAT GATGGAACCGTGTCTCTGGAGGAATGGATTCAAGGAGGAATGACAACGATTCCACTTCTTGTGCTCCTGG GCTTAGAAAATAACGTGAAGGATGATGGACAGCACGTGTGGCGACTGAAGCACTTTAACAAACCTGCCTA TTGCAACCTTTGCCTGAACATGCTGATTGGCGTGGGGAAGCAGGGCCTCTGCTGTTCCTTCTGCAAGTAC ACAGTCCATGAGCGCTGTGTGGCTCGAGCACCTCCCTCTTGCATCAAGACCTATGTGAAGTCCAAAAGGA ACACTGATGTCATGCACCATTACTGGGTTGAAGGTAACTGCCCAACCAAGTGTGATAAGTGCCACAAAAC TGTTAAATGTTACCAGGGCCTGACAGGACTGCATTGTGTTTGGTGTCAGATCACACTGCATAATAAATGT GCTTCTCATCTAAAACCTGAATGTGACTGTGGACCTTTGAAGGACCATATTTTACCACCCACAACAATCT GTCCAGTGGTACTGCAGACTCTGCCCACTTCAGGAGTTTCAGTTCCTGAGGAAAGACAATCAACAGTGAA AAAGGAAAAGAGTGGTTCCCAGCAGCCAAACAAAGTGATTGACAAGAATAAAATGCAAAGAGCCAACTCT GTTACTGTAGATGGACAAGGCCTGCAGGTCACTCCTGTGCCTGGTACTCACCCACTTTTAGTTTTTGTGA ACCCCAAAAGTGGTGGAAAACAAGGAGAACGAATTTACAGAAAATTCCAGTATCTATTAAATCCTCGTCA GGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTAAACTTTTTCCGTGATGTTCCTGACTTCAGA GTGTTAGCCTGTGGTGGAGATGGAACCGTGGGCTGGGTTTTGGATTGCATAGAAAAGGCCAATGTAGGCA AGCATCCTCCAGTTGCGATTCTGCCTCTTGGGACTGGCAATGATCTAGCAAGATGCCTGCGATGGGGAGG AGGTTACGAAGGTGAGAATCTGATGAAAATTCTAAAAGACATTGAAAACAGCACAGAAATCATGTTGGAC AGGTGGAAGTTTGAAGTCATACCTAATGACAAAGATGAGAAAGGAGACCCAGTGCCTTACAGTATCATCA ATAATTACTTTTCCATTGGCGTGGATGCCTCCATTGCACACAGATTCCACATCATGAGAGAAAAACACCC AGAGAAATTCAACAGTAGAATGAAGAACAAATTTTGGTATTTTGAGTTTGGCACATCTGAAACTTTCTCA GCCACCTGCAAGAAGCTACATGAATCTGTAGAAATAGAATGTGATGGAGTACAGATAGATTTAATAAACA TCTCTCTGGAAGGAATTGCTATTTTGAATATACCAAGCATGCATGGAGGATCCAATCTTTGGGGAGAGTC TAAGAAAAGACGAAGCCATCGACGAATAGAGAAAAAAGGGTCTGACAAAAGGACCACCGTCACAGATGCC AAAGAGTTGAAGTTTGCAAGTCAAGATCTCAGTGACCAGCTGCTGGAGGTGGTCGGCTTGGAAGGAGCCA TGGAGATGGGGCAAATATACACAGGCCTGAAAAGTGCTGGCCGGCGGCTGGCTCAGTGCTCCTGCGTGGT CATCAGGACGAGCAAGTCTCTGCCAATGCAAATTGATGGGGAGCCATGGATGCAGACCCCATGCACAATA AAAATTACACACAAGAACCAAGCCCCAATGCTGATGGGCCCGCCTCCAAAAACCGGTTTATTCTGCTCCC TCGTCAAAAGGACAAGAAACCGAAGCAAGGAATAA >OriGene 5' read for NM_004080 unedited
CCATATCCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTCCGGTGGGAGGTCTATATAA GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGCAGACTTCCAATATCAACGCGTCTTGACATAAATCAAGGACTAA GAGAGACTGAAGAAGAGCGTTAGTCATGGACAACAGTTCTTTAACACATTATTGAAATTA CAAGCATCCAAAGCAGTTTCATGTGGACAGATTGCATATTTTGAAAGCCTGAGGTATTTT ATCATGAAACATGCCATGTGGAATCTTTGAAGCATAGACCTCTGCGCAACACCTGAATAA AGAATCTTTTACCTGGTATGTGACAGAGCTTCTCACCACCACCATGACAAACCAGGAAAA ATGGGCCCACCTCAGCCCTTCGGAATTTTCCCAACTTCAGAAATATGCTGAGTATTCTAC AAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAA TCCTGAAGGGACAATAGATTTTGAAGGTTTCAAACTATTCATGAAGACATTCCTGGAAGC CGAGCTTCCTGATGATTTCACTGCACACCTTTTCATGTCATTTAGCAACAAGTTTCCTCA TTCTAGTCCAATGGTAAAAAGTAAGCCTGCTCTCCTATCAGGCGGTCTGAGAATGAATAA AGGTGCCATCACCCCTCCCCGGACTACTTCTCCTGCACATACGTGTTCCCCAGAAGTAAT CCATCTGAAGGACATTGTCTGTTACCTGTCTCTGCTTGAAAGAGGAAGACCTGAGGATAC GCTTGAGTTTATGTTTCGCCTTTAGACACGATGGGCATGGGCTCCTGGACAGCTCGGAGC TAGAGATATCATCAGTCAGATGATGCCATGTGCAAATCA >OriGene 3' read for NM_004080 unedited
NGGCCCTACTATGNACCGCGGCACGCATTCTAAGATCGATTTTTTTTTTTTTTTTTTGAG TGGTTAACGTTTTTATTTCAAAAAACAGATATTGTATATATCATCTTCTGGAAAAAATAA TCTAAGAGTACTACTAATCTAAGCTTCCATGGTTTTTAAACACAATAAGGTTATACAGAA TCTCTCAAATGTGAGTTTACTCAAACTTTATTTAAATATAGCAGAATTTAATTGGGAGTT TTAAGTGGTGATTTCTGCCTCTCACAAAATAAAAGCTCTTTTACAGGGTTTTATTTATTT ATTTTCTTAAAGTTTACAGCTTTAATATATGTTACAAGGGTTTAATCTTTTAATAGAGAT ATTATTCAGTTATTTGAAAAATAACGGAGAACATGATTCAATATTATTCAATTATTATTT AATTGTTCAGTATTGAATAATCCTTTGGGGGTTATACGTGCCATGAAAAGTACATTTTCC TTTTTTTTTTTTGAGACAGAGTCTGGCTCTGTCACCAGCTGGGGAAGTCCNTGGNGTTAT CTTGGCTCACTGGCCCCGCAGCCTTCCGAGTTCAAACCATTCTTCTGCCCTAACCTCCCG AATAGCTGGGATTACCAGGCTGTGCCACCCTGCCCGGGCAAATTTTGGATTTTTAGGAGA GACCAAGGTTCACCCATATTGGCCACGCTGGTCCTTGACCTCCTGACCCTTGGGATTCTG CCGGCCTTGGCCTCCCAAATGCCGGGATTACAAGGGGTAACCCTTGGGCCTGGCCCATTT TCCCTTTTATACCCATGGACAAAAAATTACCGTGGACCCCAAAAGGAAAGAATGGTTCTC CAAATCCTTGCTCCTTCCAGAATTACCGGTGAGATTTAAGGGAAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004080 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004080.1, NP_004071.1 |
| RefSeq Size | 3926 bp |
| RefSeq ORF | 2415 bp |
| Locus ID | 1607 |
| UniProt ID | Q9Y6T7 |
| Domains | DAGKa, DAGKc, EFh, DAG_PE-bind |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217461 | DGKB (Myc-DDK-tagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 1 |
CNY 5,800.00 |
|
| RC217461L3 | Lenti ORF clone of Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC217461L4 | Lenti ORF clone of Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 1, mGFP tagged |
CNY 8,880.00 |
|
| RG217461 | DGKB (tGFP-tagged) - Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 1 |
CNY 6,370.00 |
