ATP6V1G2 (NM_130463) Human Untagged Clone
CAT#: SC111594
ATP6V1G2 (untagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2 (ATP6V1G2), transcript variant 1
CNY 1,200.00
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ATP6G; ATP6G2; NG38; VMA10 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC111594 sequence for NM_130463 edited (data generated by NextGen Sequencing)
ATGGCCAGTCAGTCCCAAGGTATCCAGCAGCTTCTGCAAGCTGAGAAGCGGGCAGCTGAG AAGGTGGCAGATGCCAGAAAGAGGAAGGCCCGGCGACTGAAGCAGGCAAAGGAGGAGGCA CAGATGGAGGTGGAGCAATACCGCAGAGAGCGAGAGCACGAATTCCAGAGCAAGCAGCAG GCGGCCATGGGCTCCCAGGGGAACCTGTCTGCTGAGGTGGAGCAGGCTACAAGGCGCCAG GTGCAGGGCATGCAGAGCTCCCAGCAGAGAAACCGAGAGCGTGTCCTGGCCCAGCTTCTT GGCATGGTCTGCGACGTCAGGCCCCAGGTCCACCCCAACTACCGGATTTCTGCCTAG Clone variation with respect to NM_130463.3 >OriGene 5' read for NM_130463 unedited
GTAACGTCAAATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCAGA AATGGCCAGTCAGTCCCAAGTATCCAGCAGCTTCTGCAAGCTGAGAAGCGGGCAGCTGAG AAGGTGGCAGATGCCAGAAAGAGGAAGGCCCGGCGACTGAAGCAGGCAAAGGAGGAGGCA CAGATGGAGGTGGAGCAATACCGCAGAGAGCGAGAGCACGAATTCCAGAGCAAGCAGCAG GCGGCCATGGGCTCCCAGGGGAACCTGTCTGCTGAGGTGGAGCAGGCTACAAGGCGCCAG GTGCAGGGCATGCAGAGCTCCCAGCAGAGAAACCGAGAGCGTGTCCTGGCCCAGCTTCTT GGCATGGTCTGCGACGTCAGGCCCCAGGTCCACCCCAACTACCGGATTTCTGCCTAGGGC CACCGTAGGGCCTGACTCCTTCTGCCAGTTCCCTCCCTCAAAGAAATCCTCCAATCAAAA TCACCTCCCACCATAATCCCTGTCTTCTTTCCATCCCCTAGAAATCCTGGGAGGCAGGAT CCAATAATTTTCCTGTGACACTTATAAATATCCTGCTCACATCTGAATCTCCTTGTTGTT CTTTAACCCTCACTGGGACTTTGTAAACTTCCAAGTCATTCTCACCTAAACCCTCTGTGA AATTTGTAATATGGGGAAGTAGGAATGTGGAAAACATCCTGACTTCAGTGTCTGGCCGAT GTGGGTCCCTCTCTTGACCCTGTCACTTTGCTGCTGTGAAACCAGGACAAGCTACTTAAC TTGGTAGCCTCGATGTCCTCCTTTTGTGAAACTGGGGATGATTATAATGCCTACCTTGTG GAGGGTTGCTTCAATGATTTAGGATCATCCTGTAAAGTCTAACACAG >OriGene 3' read for NM_130463 unedited
TTCAACTATGNNACCGCGGCCGCATGCTAGNGATCGATTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCACCTCCCCAATTTTTTTGCC CAATAATCATTTTATTGATCCTTTTTTTATTGTTCCAAGGGGAAAAGTAAGGGGTCAAGG ATTTAAAAAGGAAGGGCTTCCTTTTAAGGGAACCCAGTTTCTTTTTAAATTTTAAAAAGC AATGACCTTTTGATAATTTTTTGGGGAAATAGGAAAGGGGGAAAATTTTTTAAAGCCAAA TTTCACCAAAAAAAAAACAAAGGGGGGCTCCAAAATGCCTTTTTTTCCCTGGAATTATAA AAAAGGGGCTAAATGTAGCCATCTCCTTTTTTTGGAAATCAAAAGGTCTTTGGAAAAAAA AAAAAACCAATTTTTCAAAAAATAACTGGGGTCACAAAATGAACAATTGGAATTAAAAAC CCCTTGATGGACTTGAGAAAACACCTTTTTAGGTTTTGACCATTGACCAGGTGGGGGTAA TAGTATCACAGGGTTGCTACTTACTGAATCACTGCTACAACATGCAAGGAACTGTGCTAA ACTTTACAAAATGATTCCTAATCATTGAACCAACCCTCACAAGGTAGGCTTTATTATCAT CCCAGTTTCACAAAGGAGGACATCGGGGCTACCAAGTTAAGTAGCTTGTCCTGGTTTCCA GCCACCAAGTGACAGGGTCAAAAGAGGGACCCCAATGGGCCAGACACTGAATCAGGATGT TTTCCACATCCTACTTCCCCTTTTTACAATTTTCACAAAGGTTTTAAGTGAAAAAGACTT GGAAATTTACAAAGTCCCGTGGGGGTTAAAGAAACACAGGAAATTCCATTGTGACCGGGT TTTTTTAAGTTCCCAGG |
Restriction Sites | NotI-NotI |
ACCN | NM_130463 |
Insert Size | 1250 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_130463.2, NP_569730.1 |
RefSeq Size | 1594 bp |
RefSeq ORF | 357 bp |
Locus ID | 534 |
UniProt ID | O95670 |
Domains | V-ATPase_G |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215424 | ATP6V1G2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2 (ATP6V1G2), transcript variant 1 |
CNY 3,990.00 |
|
RC215424L3 | Lenti-ORF clone of ATP6V1G2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2 (ATP6V1G2), transcript variant 1 |
CNY 5,890.00 |
|
RC215424L4 | Lenti-ORF clone of ATP6V1G2 (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2 (ATP6V1G2), transcript variant 1 |
CNY 5,890.00 |
|
RG215424 | ATP6V1G2 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2 (ATP6V1G2), transcript variant 1 |
CNY 4,370.00 |
其它ATP6V1G2产品