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TDP1 (NM_018319) Human Untagged Clone
CAT＃: SC111344
TDP1 (untagged)-Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1
CNY 4,536.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111344 sequence for NM_018319 edited (data generated by NextGen Sequencing)
ATGTCTCAGGAAGGCGATTATGGGAGGTGGACCATATCTAGTAGTGATGAAAGTGAGGAA GAAAAGCCAAAACCAGACAAGCCATCTACCTCTTCTCTTCTCTGTGCCAGGCAAGGAGCA GCAAATGAGCCCAGGTACACCTGTTCCGAGGCCCAGAAAGCTGCACACAAGAGGAAAATA TCACCTGTGAAATTCAGCAATACAGATTCAGTTTTACCTCCCAAAAGGCAGAAAAGCGGT TCCCAGGAGGACCTCGGCTGGTGTCTGTCCAGCAGTGATGATGAGCTGCAACCAGAAATG CCGCAGAAGCAGGCTGAGAAAGTGGTGATCAAAAAGGAGAAAGACATCTCTGCTCCCAAT GACGGCACTGCCCAAAGAACTGAAAATCATGGCGCTCCCGCCTGCCACAGGCTCAAAGAG GAGGAAGACGAGTATGAGACATCAGGGGAGGGCCAGGACATTTGGGACATGCTGGATAAA GGGAACCCCTTCCAGTTTTACCTCACTAGAGTCTCTGGAGTTAAGCCAAAGTATAACTCT GGAGCCCTCCACATCAAGGATATTTTATCTCCTTTATTTGGGACGCTTGTTTCTTCAGCT CAGTTTAACTACTGCTTTGACGTGGACTGGCTCGTAAAACAGTATCCACCAGAGTTCAGG AAGAAGCCAATCCTGCTTGTGCATGGTGATAAGCGAGAGGCTAAGGCTCACCTCCATGCC CAGGCCAAGCCTTACGAGAACATCTCTCTCTGCCAGGCAAAGTTGGATATTGCGTTTGGA ACACACCACACGAAAATGATGCTGCTGCTCTATGAAGAAGGCCTCCGGGTTGTCATACAC ACCTCCAACCTCATCCATGCTGACTGGCACCAGAAAACTCAAGGAATATGGTTGAGCCCC TTATACCCACGAATTGCTGATGGAACCCACAAATCTGGAGAGTCGCCAACACATTTTAAA GCTGATCTCATCAGTTACTTGATGGCTTATAATGCCCCTTCTCTCAAGGAGTGGATAGAT GTCATTCACAAGCACGATCTCTCTGAAACAAATGTTTATCTTATTGGTTCAACCCCAGGA CGCTTTCAAGGAAGTCAAAAAGATAATTGGGGACATTTTAGACTTAAGAAGCTTCTGAAA GACCATGCCTCATCCATGCCTAACGCAGAGTCCTGGCCTGTCGTAGGTCAGTTTTCAAGC GTTGGCTCCTTGGGAGCCGATGAATCAAAGTGGTTATGTTCTGAGTTTAAAGAGAGCATG CTGACACTGGGGAAGGAAAGCAAGACTCCAGGAAAAAGCTCTGTTCCTCTTTACTTGATC TATCCTTCTGTGGAAAATGTGCGGACCAGTTTAGAAGGATATCCTGCTGGGGGCTCTCTT CCCTATAGCATCCAGACAGCTGAAAAACAGAATTGGCTGCATTCCTATTTTCACAAATGG TCAGCTGAGACTTCTGGCCGCAGCAATGCCATGCCACATATTAAGACATATATGAGGCCT TCTCCAGACTTCAGTAAAATTGCTTGGTTCCTTGTCACAAGCGCAAATCTGTCCAAGGCT GCCTGGGGAGCATTGGAGAAGAATGGCACCCAGCTGATGATCCGCTCCTACGAGCTCGGG GTCCTTTTCCTCCCTTCAGCATTTGGTCTAGACAGTTTCAAAGTGAAACAGAAGTTCTTC GCTGGCAGCCAGGAGCCAATGGCCACCTTTCCTGTGCCATATGATTTGCCTCCAGAACTG TATGGAAGTAAAGATCGGCCATGGATATGGAACATTCCTTATGTCAAAGCACCGGATACG CATGGGAACATGTGGGTGCCCTCCTGA Clone variation with respect to NM_018319.3 >OriGene 5' read for NM_018319 unedited
NGGGTCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCGAGGCA AGCGTTGGTTCTGTGCGCCTCAGGAGTATAATGTCTCAGGAAGGCGATTATGGGAGGTGG ACCATATCTAGTAGTGATGAAAGTGAGGAAGAAAAGCCAAAACCAGACAAGCCATCTACC TCTTCTCTTCTCTGTGCCAGGCAAGGAGCAGCAAATGAGCCCAGGTACACCTGTTCCGAG GCCCAGAAAGCTGCACACAAGAGGAAAATATCACCTGTGAAATTCAGCAATACAGATTCA GTTTTACCTCCCAAAAGGCAGAAAAGCGGTTCCCAGGAGGACCTCGGCTGGTGTCTGTCC AGCAGTGATGATGAGCTGCAACCAGAAATGCCGCAGAAGCAGGCTGAGAAAGTGGTGATC AAAAAGGAGAAAGACATCTCTGCTCCCAATGACGGCACTGCCCAAAGAACTGAAAATCAT GGCGCTCCCGCCTGCCACAGGCTCAAAGAGGAGGAAGACGAGTATGAGACATCAGGGGAG GGCCAGGACATTTGGGACATGCTGGATAAAGGGAACCCCTTCCAGTTTTACCTCACTAGA GTCTCTGGAGTTAAGCCAAAGTATAACTCTGGAGCCCTCCACATCAAGGATATTTTATCT CCTTTATTTGGGACGCTTGTTTCTTCAGCTCAGTTTAACTACTGCTTTGACGTGGACTGG CTCGTAAAACAGTATCCACCAGAGTTCANGAAGAAGCCAATCCTGCTTGTGCATGGTGAT AAGCGAGAGGCTAAGGCTCACCTNCATGCCCCAGCCAAGCCTTACGAGAACATCTCTCTC TGCCAGNCAAAGNTGGGATATTGCGTTNGGAACACACCACACGAAAATGATGCTGCTGCT CTATGAAAANGGCCTCNCGGNNTGTCATACACACCTNNCAACTCATNCATGC >OriGene 3' read for NM_018319 unedited
AAATAGGGGTTNCGATTCCTTTTTAAAAAGACAGACACACAAAATACATACAACACCCCA TAAATGTCCTTTACAGAATCCCAGTATGTACAACAGATACAGCAGAGCCTGTCTGTTGGA AGGTGGGAGGCCCTGCAGCCCTGTCTCCCTGCCTCCTGCTCTATCGCCACACCCCCACTG CAGCCTACAAGGTTCCTAAGGTACTTTTGGAAAATCCTAGGCCTCTGAGGAACAGTTTGA AAGACTACTTTAGAAAATCTTTCAAGACTAACGTTCAACTCTATCCTAAATTATAAGATA AAGAGGTTGATGACAAACTGGAAATCAGCCTCATGCAGAAGAAAACAAACAGGGTCTAAA GCACCCTAGGGAGAACACAATGTACACAAAGCACAAAGACACTCAAGCTCTGAGCCCCGC TCACTGTGTGACCCTGGGGACATCCTTAACCTTCTAGGCCTTAGCTTCCTCACTTGTGAA TTTAAGGCATCAAATTAAGTCACCTTTTAGGATTTTTTCCCAAAATACTTGTGTGCTTTT GTATGTTTCTATGAGCTGCTTCCTCTGAAAGTTTCTGGGGATAGAAGAGACACCCATTTA CCCTAAGCAGGTGTTTGGGTTTGGTGGGACCTGTCTGTCAGCAGTGAGGCAGCCAGCATG GTGCCCGGGAGAGATGAGAAACCTCCTAAAAGCTAAGCTGAAAGCTATGAAAAGCCGCTG TGAGGACAGTCAATACAATTCTTAACTGCAGGGGGCTGAAACAAGCCATTGGTCTCAAAG ACAGATCCCTAGATTCACCTCCAGCACCCCAGAAAACTTACTTCAAAAACCTTAAAGAAG GCATCCGTGGAAAAAGAAAAGATCGTGTTCTTACCGGGAAAAATTTCTGCGGGCAAGAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018319 |
| Insert Size | 3780 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018319.3, NP_060789.2 |
| RefSeq Size | 3763 bp |
| RefSeq ORF | 1827 bp |
| Locus ID | 55775 |
| UniProt ID | Q9NUW8 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016] Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1 and 2 encode the same isoform. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Tyrosyl-DNA phosphodiesterase 1 and topoisomerase I activities as predictive indicators for Glioblastoma susceptibility to genotoxic agents
,Wang, W;Rodriguez-Silva, M;de la Rocha, AMA;,
bioRxiv
[TDP1]
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Other Versions
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|---|---|---|---|
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| RC214927L1 | Lenti ORF clone of Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1, Myc-DDK-tagged |
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| RC214927L2 | Lenti ORF clone of Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1, mGFP tagged |
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| RC214927L3 | Lenti ORF clone of Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214927L4 | Lenti ORF clone of Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
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| RG214927 | TDP1 (tGFP-tagged) - Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1 |
CNY 6,128.00 |
