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VPS24 (CHMP3) (NM_016079) Human Untagged Clone
CAT＃: SC111287
CHMP3 (untagged)-Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGI-149; NEDF; VPS24 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111287 sequence for NM_016079 edited (data generated by NextGen Sequencing)
ATGGGGCTGTTTGGAAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCA TTGAAGATAAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAA GAAGAAAAAGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGC ATAGTTCTGGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCC AAAGCCCACATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTG GCTGGTTCCCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATT CCAGAGATTCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATA GAGGAGATGTTAGAGGACACTTTTGAAAGCATGGACGATCAGGAAGAAATGGAGGAAGAA GCAGAAATGGAAATTGACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCA CCCAGTAAAGTGACTGATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCA GAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTC CGCAGCTAG Clone variation with respect to NM_016079.3 246 a=>c >OriGene 5' read for NM_016079 unedited
ATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGTTCAGTTCGTCATGGGGCT GTTTGGAAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCATTGAAGAT AAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAA AGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCT GGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCCAAAGCCCA CATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTC CCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGAT TCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATAGAGGAGAT GTTAGAGGACACTTTTGAAAGCATGGACGATCAGGAAGAAATGGAGGAAGAAGCAGAAAT GGAAATTGACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAA AGTGACTGATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCANACGATGA GGAGGAGGAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTA GGGGCTGCCTACCCCGCTGGGTGTGCACACACTCCTCTCAAGAGCTGCCATTNTATGTGT CTCTTGCACTACACCNTCTGTGTGAGGACTACCATTTTGGAGAAGGNTCTGTNTGNCTCT TTTCATTNCTCTGCCCAGTTNNTGGGATCGCANAAGGGATNGNTCTTATNAAAGTGGCAT AAATAAATGCATCATTTNNNTAGAGATAGACAGATTTATCTTATTNGTGGGGAGGGGAAA GAAATNCATCTGCTCATGAAGCCTTNCTGAAAAT >OriGene 3' read for NM_016079 unedited
NNTTTTAGCTTTGGACCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTGN GAGAGAAGGATCTTGCTATGTTGCCCAGGCTGGTCTTGAACTCCTATTCTCAAGAGAGCC TCCTGCCTCAGCCTTGTAAAGCACTGGGATTATAGGCATGAACCACCGCACCCAGCCAAG ATTGCCATTTTGTATGATGAGACTGGAAGGACCCCATTGTTTCAGGATTTTGCTACAATA TACAAAAAACAATCTGTGAGACAGTGGCTGGGCTTTTTTCCTGCCTGATTAGTTCAGTGC ACATACAACTTGGACCAGAGGATCTGGGTTTGAATCCCATCTCTGATACTTCCCAAACTG AGCTGTTTTCCTTATTTGTAAAGACTAAGATCGCGTATGTCAAAGAGCTCTGTAAACTCT CAACACATACAAAGTACTACTGCTGAAATGATTTATAAATGTATCTTAAGAATGTCCCAG ATGAGTGAATGTTGGTTATGATGCATGACCACACCTTAGTGTGCTCCTGGTCAGCTCCTG ACTTTGAGAAAACACAGATAGGAAGGCCCATGTCCCACGCACAAGACAACCAGCTCGAAG CAGGGACAGGCAATGAGAGGTGAAAGAGACCTAGTACTACAGCTGTCTCATCTGGGTGCA ACTTGCAATGGGAAGGAAGGGACAGAAGCTCTAAATAGACCCACATGCACACCCCAGGAC ATAATGCAGCCACCAGCCAACAGGACAAGCAGCACATTTCCCCAGCATCCTCTTGTTTAA ATAGAAACAGAATGGCTGCCATACCATGTCTGTGAAAGCCGGGAGCAGCAACCACAGAGC AAAGTCACTTCTGCTGAGCTCCCAAATCCATATGCCTGGCCTAGCAACTCTTTCCACACC AA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016079 |
| Insert Size | 2080 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016079.2, NP_057163.1 |
| RefSeq Size | 3174 bp |
| RefSeq ORF | 669 bp |
| Locus ID | 51652 |
| UniProt ID | Q9Y3E7 |
| Domains | DUF279 |
| Protein Pathways | Endocytosis |
| Gene Summary | This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as Vps24alpha). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220006 | CHMP3 (Myc-DDK-tagged)-Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1 |
CNY 2,400.00 |
|
| RC220006L1 | Lenti ORF clone of Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC220006L2 | Lenti ORF clone of Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC220006L3 | Lenti ORF clone of Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220006L4 | Lenti ORF clone of Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG220006 | CHMP3 (tGFP-tagged) - Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1 |
CNY 4,000.00 |
