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LIPT1 (NM_145199) Human Untagged Clone
CAT＃: SC111284
LIPT1 (untagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 5
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LIPT1D |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111284 sequence for NM_145199 edited (data generated by NextGen Sequencing)
ATGCTGATCCCATTTTCAATGAAGAATTGCTTCCAGTTACTTTGTAACTGCCAGGTCCCA GCAGCTGGCTTTAAAAAAACAGTAAAAAATGGGCTCATTTTACAGTCAATTTCCAATGAT GTCTATCAAAATCTGGCTGTGGAAGACTGGATCCATGACCATATGAATCTAGAAGGCAAA CCAATTCTATTCTTTTGGCAGAATTCTCCCTCTGTTGTAATTGGTAGGCATCAAAATCCT TGGCAGGAATGTAACCTGAATCTAATGAGAGAAGAAGGTATAAAACTGGCTCGGAGAAGA AGTGGAGGAGGAACAGTCTACCATGATATGGGTAATATCAATTTGACTTTCTTTACAACC AAAAAAAAGTATGATAGAATGGAAAATCTGAAATTAATTGTGAGAGCTCTGAATGCTGTC CAACCCCAGCTGGATGTGCAGGCTACCAAAAGATTTGACCTTTTACTTGATGGACAGTTT AAAATCTCAGGAACAGCTTCTAAGATCGGCCGGACTACTGCCTATCACCATTGCACTTTA TTATGTAGTACTGATGGGACGTTCCTGTCTTCTTTGCTAAAGAGCCCTTACCAAGGGATC AGGAGCAATGCCACTGCTAGCATACCTTCCTTAGTGAAAAATCTTTTGGAAAAGGATCCC ACTCTGACCTGTGAAGTACTAATGAATGCTGTTGCTACAGAGTATGCTGCCTATCATCAA ATTGATAATCACATTCACCTAATAAACCCAACGGATGAGACACTGTTTCCTGGAATAAAT AGCAAAGCCAAAGAACTGCAAACTTGGGAGTGGATATATGGCAAAACTCCAAAGTTTAGT ATAAATACTTCCTTTCATGTGTTATATGAACAGTCACACTTGGAAATTAAAGTATTCATA GACATAAAGAATGGAAGAATTGAAATTTGTAATATTGAAGCACCTGATCATTGGTTGCCA TTGGAAATACGTGACAAATTAAATTCAAGTCTTATTGGCAGTAAGTTTTGCCCAACTGAA ACTACCATGCTAACAAATATATTACTTAGAACATGTCCACAAGACCACAAACTAAACAGT AAATGGAATATTCTCTGTGAAAAAATTAAGGGAATAATGTGA Clone variation with respect to NM_145199.2 565 t=>c;711 t=>c >OriGene 5' read for NM_145199 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCACGAGGCCGTGGGTACGAC CGGAAGCCGCAGCATGCTGATCCCATTTTCAATGAAGAATTGCTTCCAGTTACTTTGTAA CTGCCAGGTCCCAGCAGCTGGCTTTAAAAAAACAGTAAAAAATGGGCTCATTTTACAGTC AATTTCCAATGATGTCTATCAAAATCTGGCTGTGGAAGACTGGATCCATGACCATATGAA TCTAGAAGGCAAACCAATTCTATTCTTTTGGCAGAATTCTCCCTCTGTTGTAATTGGTAG GCATCAAAATCCTTGGCAGGAATGTAACCTGAATCTAATGAGAGAAGAAGGTATAAAACT GGCTCGGAGAAGAAGTGGAGGAGGAACAGTCTACCATGATATGGGTAATATCAATTTGAC TTTCTTTACAACCAAAAAAAAGTATGATAGAATGGAAAATCTGAAATTAATTGTGAGAGC TCTGAATGCTGTCCAACCCCAGCTGGATGTGCAGGCTACCAAAAGATTTGACCTTTTACT TGATGGACAGTTTAAAATCTCAGGAACAGCTTCTAAGATCGGCCGGACTACTGCCTATCA CCATTGCACTTTATNNATGTAGTACTGTAGGGGGACGTTCCCTGGTCTTCTTTGCTAAAG AGCCCTTCCANNAGGGATCAGGAGCATGCCACTGCTAGCATACCTTCCTTAGTGAAAAAT CTTTTGGAAAAAAGGATCCCACTCTGACCTGNNNGAGTACTAATGGAATGGCTGGTGCTA CAGAAGTATGCTGCCTATCATCANATTGATAATCACATTCACCTAATAACCCAACGGATG ANACACTGGNTTCCCTGGAATAAATAGCNAGCCAAACTGCTNTTTGGGNAGAGGGGGATT TTTGCAAACCTCCCCAGGGTTAGATAAAACTTCTCTTCTCGGGGGATAAGGAGACAGACA CTGNAAAATTAAAGGTTCTACTTAGAA >OriGene 3' read for NM_145199 unedited
CTTGAACGCCGCCGTTTCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTTTCTAATTGAA ACTGTATTAAGACATTTACTTGGAATCACATTATTCCCTTAATTTTTTCACAGAGAATAT TCCATTTACTGTTTAGTTTGTGGTCTTGTGGACATGTTCTAAGTAATATATTTGTTAGCA TGGTAGTTTCAGTTGGGCAAAACTTACTGCCAATAAGACTTGAATTTAATTTGTCACGTA TTTCCAATGGCAACCAATGATCAGGTGCTTCAATATTACAAATTTCAATTCTTCCATTCT TTATGTCTATGAATACTTTAATTTCCAAGTGTGACTGTTCATATAACACATGAAAGGAAG TATTTATACTAAACTTTGGAGTTTTGCCATATATCCACTCCCAAGTTTGCAGTTCTTTGG CTTTGCTATTTATTCCAGGAAACAGTGTCTCATCCGTTGGGTTTATTAGGTGAATGTGAT TATCAATTTGATGATAGGCAGCATACTCTGTAGCAACAGCATTCATTAGTACTTCACAGG TCAGAGTGGGATCCTTTTCCAAAAGATTTTTCACTAAGGAAGGTATGCTAGCAGTGGCAT TGCTCCTGATCCCTTGGTAAGGGCTCTTTAGCAAAGAAGACAGGAACGTCCCATCAGTAC TACATAATAAAGTGCAATGGTGATAGGCAGTAGTCCGGCCGATCTTAGAGCTGTTCCTGA GATTTAAAACTGCCATCAAGTAAAGGTCAAATCTTTTGGTAGCCTGCACATCCAGCTGGG GTTGGACAGCATTCAGAGCTCTCACAATTAATTTCAGATTTTCCATCCTACCATAACTTT TTTTGGTTGAAAGGAAGTCACATTGTATTACCCATATCATGGAAACTTGTCCTCCTCCAT TCTTTCTCGAAGCACGTTTATACTTCTTTTTCATAAATCCAGGTACTTTCTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145199 |
| Insert Size | 1250 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145199.1, NP_660200.1 |
| RefSeq Size | 1288 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 51601 |
| UniProt ID | Q9Y234 |
| Domains | BPL_LipA_LipB |
| Protein Pathways | Lipoic acid metabolism, Metabolic pathways |
| Gene Summary | The process of transferring lipoic acid to proteins is a two-step process. The first step is the activation of lipoic acid by lipoate-activating enzyme to form lipoyl-AMP. For the second step, the protein encoded by this gene transfers the lipoyl moiety to apoproteins. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 13. Read-through transcription also exists between this gene and the neighboring downstream mitochondrial ribosomal protein L30 (MRPL30) gene. [provided by RefSeq, Mar 2011] Transcript Variant: This variant (5) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 3-6 all encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216793 | LIPT1 (Myc-DDK-tagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 5 |
CNY 5,488.00 |
|
| RC216793L3 | Lenti-ORF clone of LIPT1 (Myc-DDK-tagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 5 |
CNY 5,890.00 |
|
| RC216793L4 | Lenti-ORF clone of LIPT1 (mGFP-tagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 5 |
CNY 5,890.00 |
|
| RG216793 | LIPT1 (tGFP-tagged) - Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 5 |
CNY 7,088.00 |
