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Argonaute 2 (AGO2) (NM_012154) Human Untagged Clone
CAT＃: SC111241
AGO2 (untagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1
CNY 6,296.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CASC7; EIF2C2; LESKRES; LINC00980; PPD; Q10 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_012154, the custom clone sequence may differ by one or more nucleotides
ATGTACTCGGGAGCCGGCCCCGCACTTGCACCTCCTGCGCCGCCGCCCCCCATCCAAGGATATGCCTTCA AGCCTCCACCTAGACCCGACTTTGGGACCTCCGGGAGAACAATCAAATTACAGGCCAATTTCTTCGAAAT GGACATCCCCAAAATTGACATCTATCATTATGAATTGGATATCAAGCCAGAGAAGTGCCCGAGGAGAGTT AACAGGGAAATCGTGGAACACATGGTCCAGCACTTTAAAACACAGATCTTTGGGGATCGGAAGCCCGTGT TTGACGGCAGGAAGAATCTATACACAGCCATGCCCCTTCCGATTGGGAGGGACAAGGTGGAGCTGGAGGT CACGCTGCCAGGAGAAGGCAAGGATCGCATCTTCAAGGTGTCCATCAAGTGGGTGTCCTGCGTGAGCTTG CAGGCGTTACACGATGCACTTTCAGGGCGGCTGCCCAGCGTCCCTTTTGAGACGATCCAGGCCCTGGACG TGGTCATGAGGCACTTGCCATCCATGAGGTACACCCCCGTGGGCCGCTCCTTCTTCACCGCGTCCGAAGG CTGCTCTAACCCTCTTGGCGGGGGCCGAGAAGTGTGGTTTGGCTTCCATCAGTCCGTCCGGCCTTCTCTC TGGAAAATGATGCTGAATATTGATGTGTCAGCAACAGCGTTTTACAAGGCACAGCCAGTAATCGAGTTTG TTTGTGAAGTTTTGGATTTTAAAAGTATTGAAGAACAACAAAAACCTCTGACAGATTCCCAAAGGGTAAA GTTTACCAAAGAAATTAAAGGTCTAAAGGTGGAGATAACGCACTGTGGGCAGATGAAGAGGAAGTACCGC GTCTGCAATGTGACCCGGCGGCCCGCCAGTCACCAAACATTCCCGCTGCAGCAGGAGAGCGGGCAGACGG TGGAGTGCACGGTGGCCCAGTATTTCAAGGACAGGCACAAGTTGGTTCTGCGCTACCCCCACCTCCCATG TTTACAAGTCGGACAGGAGCAGAAACACACCTACCTTCCCCTGGAGGTCTGTAACATTGTGGCAGGACAA AGATGTATTAAAAAATTAACGGACAATCAGACCTCAACCATGATCAGAGCGACTGCTAGGTCGGCGCCCG ATCGGCAAGAAGAGATTAGCAAATTGATGCGAAGTGCAAGTTTCAACACAGATCCATACGTCCGTGAATT TGGAATCATGGTCAAAGATGAGATGACAGACGTGACTGGGCGGGTGCTGCAGCCGCCCTCCATCCTCTAC GGGGGCAGGAATAAAGCTATTGCGACCCCTGTCCAGGGCGTCTGGGACATGCGGAACAAGCAGTTCCACA CGGGCATCGAGATCAAGGTGTGGGCCATTGCGTGCTTCGCCCCCCAGCGCCAGTGCACGGAAGTCCATCT GAAGTCCTTCACAGAGCAGCTCAGAAAGATCTCGAGAGACGCCGGCATGCCCATCCAGGGCCAGCCGTGC TTCTGCAAATACGCGCAGGGGGCGGACAGCGTGGAGCCCATGTTCCGGCACCTGAAGAACACGTATGCGG GCCTGCAGCTGGTGGTGGTCATCCTGCCCGGCAAGACGCCCGTGTACGCCGAGGTCAAGCGCGTGGGAGA CACGGTGCTGGGGATGGCCACGCAGTGCGTGCAGATGAAGAACGTGCAGAGGACCACGCCACAGACCCTG TCCAACCTCTGCCTGAAGATCAACGTCAAGCTGGGAGGCGTGAACAACATCCTGCTGCCCCAGGGCAGGC CGCCGGTGTTCCAGCAGCCCGTCATCTTTCTGGGAGCAGACGTCACTCACCCCCCCGCCGGGGATGGGAA GAAGCCCTCCATTGCCGCCGTGGTGGGCAGCATGGACGCCCACCCCAATCGCTACTGCGCCACCGTGCGC GTGCAGCAGCACCGGCAGGAGATCATACAAGACCTGGCCGCCATGGTCCGCGAGCTCCTCATCCAGTTCT ACAAGTCCACGCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGACGGTGTCTCTGAAGGCCAGTTCCA GCAGGTTCTCCACCACGAGTTGCTGGCCATCCGTGAGGCCTGTATCAAGCTAGAAAAAGACTACCAGCCC GGGATCACCTTCATCGTGGTGCAGAAGAGGCACCACACCCGGCTCTTCTGCACTGACAAGAACGAGCGGG TTGGGAAAAGTGGAAACATTCCAGCAGGCACGACTGTGGACACGAAAATCACCCACCCCACCGAGTTCGA CTTCTACCTGTGTAGTCACGCTGGCATCCAGGGGACAAGCAGGCCTTCGCACTATCACGTCCTCTGGGAC GACAATCGTTTCTCCTCTGATGAGCTGCAGATCCTAACCTACCAGCTGTGTCACACCTACGTGCGCTGCA CACGCTCCGTGTCCATCCCAGCGCCAGCATACTACGCTCACCTGGTGGCCTTCCGGGCCAGGTACCACCT GGTGGATAAGGAACATGACAGTGCTGAAGGAAGCCATACCTCTGGGCAGAGTAACGGGCGAGACCACCAA GCACTGGCCAAGGCGGTCCAGGTTCACCAAGACACTCTGCGCACCATGTACTTTGCTTGA >OriGene 5' read for NM_012154 unedited
TCCCGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT TCGGCACGAGGCCGCGCCGGGGCGGATCGTGCGGCCGGCGGCTCCCTCGCGGCTCGCGGC GTCGGGGCCCGTGGCGCGCGCGCGGCCGCCCCTCGGCCCCGGAGCCCCTCGGCGGCGCCA CCATGTACTCGGGAGCCGGCCCCGCACTTGCACCTCCTGCGCCGCCGCCCCCCATCCAAG GATATGCCTTCAAGCCTCCACCTAGACCCGACTTTGGGACCTCCGGGAGAACAATCAAAT TACAGGCCAATTTCTTCGAAATGGACATCCCCAAAATTGACATCTATCATTATGAATTGG ATATCAAGCCAGAGAAGTGCCCGAGGAGAGTTAACAGGGAAATCGTGGAACACATGGTCC AGCACTTTAAAACACAGATCTTTGGGGATCGGAAGCCCGTGTTTGACGGCAGGAAGAATC TATACACAGCCATGCCCCTTNCGATTGGGAGGGACAAGGTGGAGCTGGAGGTCACGCTGC CAGGAGAGGGCAGGATCGCATCTTCAAGGGTGTCCATCAGTGGGTGTCCTGCGTGAGCTT GCAGGGCGTACACGATGCACTTTCAGGGNCGCTGCCCAGCGTCCCTTTTGAGACGATCCA CGCCCCTGGACGTGGTCATGAGGCACTTGCCATCCATTGAGTACACCCCCGTGGGCCCGC TCCTTCTTCACCGCGTCCGAAGGCTGCTCTAACCCTCCTGGCCGGGGCCCGAAANNGTGG NTTTGGCTTNCATCAGTCCGGCCGGGCCTTTTTCTGGGAAATGATGCTGAAATTGAAGGG TCAACACCAGGGTTTACAAGGCCAACCAGTAACCAGTTTGTTGTGAAGTTTGAATT >OriGene 3' read for NM_012154 unedited
CCTTGGCGATGGCACTCTCCATGCCAGNATAGCACTGGGTGAGGGTCACAGGGCTGCCAC CCGGGTATCTGTTGCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTT TTTTTTTTTTTTAAATACATTTTTTATTAAAATGGCACTTGTCTGCCAACCTCTCTGGAC CTGGAAATGCTGTAAACACACGGGGCCCACCTTCTGCCACTGCTCCTTATTTCAAGCCGG GTGAAGGGCGGTGCTGGCAGAACACGGCACCTGCGGACGCCTCTGCATGGCCACCGGCCC TCAGTCGAGGAACTTGGGTGAGCCACGCCAGGGGCACACGAGCATCGCCTGGAAATCAAC TTTAGTGACAACATCAGAATGTTCAAGGCTTTAAAACCACACTTGTGTTTTGTGTTGCTT TCACTCTCAGAAGATTCACAGCTAGTTTGAGCCCATCAATTTCATAGAGAATCATGTATT AAAAACAAGTAAATTGAATGACCAAATAAATTAAAAATGTTTTAAAGCCCTGAGTTCATA GACTGGTTTTAAGAGATTTTTAAGACACACGGGACTCTGTTTTAATAAGCAGCTTATAGA TTTTAGCAAACCATATTTCCTATGACATTGGGTTCTCATACAGGTCTGCAGTTCAAAATC CAAGTTTGAAATCTGGGACGGAAGGCATTCTGGAAAACGGAGAATCTAATAAAATCAATG ACGTCTCATGTTCGATGCTGGCTGTCACGGAAGGGCTGGCCATCTGTTGGTCTGAGTGTA NCTGGTCTCGTGAATCCCCACTCGTACACAATCGCTAAACACTAANACATGTCAAGCANA GTACATGGTGCGCANAGTGTCTTGGTGAACCTGNACCGNCTTGGCCAGTGCTTGGTGGTC TCGNCCGTACTCTGCCCAGAGTATGGCTTCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012154 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012154.2, NP_036286.2 |
| RefSeq Size | 3567 bp |
| RefSeq ORF | 2580 bp |
| Locus ID | 27161 |
| UniProt ID | Q9UKV8 |
| Domains | PAZ, Piwi |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Asymmetric RNA duplexes mediate RNA interference in mammalian cells
,Xiangao Sun, Harry A Rogoff, Chiang J Li,
Nature Biotechnology 26, 1379 - 1382 (01 Dec 2008), doi: 10.1038/nbt.1512, Research
[AGO2]
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Other Versions
| SKU | Description | Size | Price |
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| RC218078 | AGO2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1 |
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| RC218078L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, Myc-DDK-tagged |
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| RC218078L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, mGFP tagged |
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| RC218078L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, Myc-DDK-tagged |
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| RC218078L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, mGFP tagged |
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| RG218078 | AGO2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1 |
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