MAD2L2 (NM_006341) Human Untagged Clone
CAT#: SC111153
MAD2L2 (untagged)-Human MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2), transcript variant 2
CNY 2,400.00
CNY 2,950.00
Cited in 1 publication. |
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | FANCV; MAD2B; POLZ2; REV7 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC111153 sequence for NM_006341 edited (data generated by NextGen Sequencing)
ATGACCACGCTCACACGACAAGACCTCAACTTTGGCCAAGTGGTGGCCGATGTGCTCTGC GAGTTCCTGGAGGTGGCTGTGCATCTCATCCTCTACGTGCGCGAGGTCTACCCCGTGGGC ATCTTCCAGAAACGCAAGAAGTACAACGTGCCGGTCCAGATGTCCTGCCACCCGGAGCTG AATCAGTATATCCAGGACACGCTGCACTGCGTCAAGCCACTCCTGGAGAAGAATGATGTG GAGAAAGTGGTGGTGGTGATTTTGGATAAAGAGCACCGCCCAGTGGAGAAATTCGTCTTT GAGATCACCCAGCCTCCACTGCTGTCCATCAGCTCAGACTCGCTGTTGTCTCATGTGGAG CAGCTGCTCCGGGCCTTCATCCTGAAGATCAGCGTGTGCGATGCCGTCCTGGACCACAAC CCCCCAGGCTGTACCTTCACAGTCCTGGTGCACACGAGAGAAGCCGCCACTCGCAACATG GAGAAGATCCAGGTCATCAAGGATTTCCCCTGGATCCTGGCGGATGAGCAGGATGTCCAC ATGCATGACCCCCGGCTGATACCACTAAAAACCATGACGTCGGACATTTTAAAGATGCAG CTTTACGTGGAAGAGCGCGCTCATAAAGGCAGCTGA Clone variation with respect to NM_006341.3 >OriGene 5' read for NM_006341 unedited
GATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCGCGATCGGTC CGGTCGGTGGCTCCCCGCGGCGGGGCCGGGCCCGATCTCGGGCGGGAACCGAGCGCAGAG CCGGTAGCGGGAAGGATGACCACGCTCACACGACAAGACCTCAACTTTGGCCAAGTGGTG GCCGATGTGCTCTGCGAGTTCCTGGAGGTGGCTGTGCATCTCATCCTCTACGTGCGCGAG GTCTACCCCGTGGGCATCTTCCAGAAACGCAAGAAGTACAACGTGCCGGTCCAGATGTCC TGCCACCCGGAGCTGAATCAGTATATCCAGGACACGCTGCACTGCGTCAAGCCACTCCTG GAGAAGAATGATGTGGAGAAAGTGGTGGTGGTGATTTTGGATAAAGAGCACCGCCCAGTG GAGAAATTCGTCTTTGAGATCACCCAGCCTCCACTGCTGTCCATCAGCTCAGACTCGCTG TTGTCTCATGTGGAGCAGCTGCTCCGGGCCTTCATCCTGAAGATCAGCGTGTGCGATGCC GTCCTGGACCACAACCCCCCAGGCTGTACCTTCACAGTCCTGGTGCACACGAGAGAAGCC GCCACTCGCAACATGGAGAAGATCCAGGTCATCAAGGATTTCCCCTGGATCCTGGCGGAT GAGCAGGATGTCCACATGCATGACCCCCGGCTGATACCACTAAAAACCATGACGTCGGAC ATTNTAAAGATGCAGCTNTACGTGGAAGAGCGCGCTCATAAAGGCAGCTGNAGGGGCACC TGCCACCCCACTGATGCCCAAACTGTCAGACTTTGNGGNATCCCCGCCTAGGGCAGTGCT GCATGGCTGCCCTGATTCCAAGTGCTCTTATCGCCTCTGTGTGTGGATCGCCCGNCCCAG CCCGGNGCCGCTCAGTCTGCTTGGAGGATGCTNNCCCAGNAGGCAGTGAGGGATGCCGCA CCTCNACTCTCACCTNCTGGGN >OriGene 3' read for NM_006341 unedited
GCCGCTTTTAGAGTCGAGNAANTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA AGCCCAGCCCTTGGGGCCCCTTTATTGAAACAACTCACAGCACAGTTTTTGAAACAAACA GGGTTGGCCGGGGGAACCCCAGAAGGCTGAAAATTCAAGGTTGCGGCATCCCTCACTGCC CTCCTGGGGGAGGCATCCTCCAAGCAAACCTGAGCGGCCCCGGGCTGGGGCGGGCGATCC ACACACAGAGGCGATAAAACCACTTGGAATCAGGGCAGCCATGCAGCACTGCCCTAGGCG GGGATCCCCCAAAGTCTGACAGTTTGGGCATAAGGGGGGTGGCAGGTGCCCCCTCAGCTG CCTTTATGACCGCGCTCTTCCACGTAAAGCTGCATCTTTAAAATGCCCGACGTAATGGTT TTTAGGGGTATCAGCCGGGGGTCATGCATGTGGACATCCTGCTCATCCGCCAGGATCCAG GGGAAATCCTTGATGACCTGGATCTTCTCCATGTTGCGAGAGGCGGCTTCTCTCGTGTGC ACCAGGACTGTGAAGGTACAGCCTGGGGGGTTGTGGCCCAGGACGGCATCGCACACGCTG ATCTTAAGGATGAAGGCCCGGACCAGCTGCTCCACATGAGACAACAGCGAGTTCTGATCT GATGCACAGCAGTGGAGGCTGGGTGATCCCAAAGACAAATTTCTCCACTGGGCGGAGCTC TTTTTCCAAATTACCACCACAATTTTTTCCAATAATCCTTTTCCAGAATTGGTTAGAACA ATGCAACGGGTCCTGATATTCTGATTAACCTCCGGTTGAAAGAATTCTGAACCCGGCACC TGTCTTCTTGCGTTCTGGAAAATCCCCAGGGGTTACCTCCCCCCCTTAAGGTGAAAAGCA CCGCCCCTCCAGAACTCCAAGGACATGCGCCCACTTGCCCAAGTTAAGGCTTGTTGGGAA ACGGGCCATCTCCCCGCCACCCTCTCCCCG |
Restriction Sites | NotI-NotI |
ACCN | NM_006341 |
Insert Size | 1050 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006341.2, NP_006332.2 |
RefSeq Size | 1056 bp |
RefSeq ORF | 636 bp |
Locus ID | 10459 |
UniProt ID | Q9UI95 |
Domains | HORMA |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
Gene Summary | The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The Vital Role of Polymerase and REV1 in Mutagenic, but Not Correct, DNA Synthesis across Benzo[a]pyrene-dG and Recruitment of Polymerase by REV1 to Replication-stalled Site
,Keiji Hashimoto, Youngjin Cho, In-Young Yang, Jun-ichi Akagi, Eiji Ohashi, Satoshi Tateishi, Niels de Wind, Fumio Hanaoka, Haruo Ohmori, and Masaaki Moriya,
J. Biol. Chem., Mar 2012; 287: 9613 - 9622.
[MAD2L2]
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Other Versions
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RC203932 | MAD2L2 (Myc-DDK-tagged)-Human MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2), transcript variant 2 |
CNY 2,400.00 |
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RC203932L3 | Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2), transcript variant 2, Myc-DDK-tagged |
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RC203932L4 | Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2), transcript variant 2, mGFP tagged |
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RG203932 | MAD2L2 (tGFP-tagged) - Human MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2), transcript variant 2 |
CNY 4,000.00 |