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Visfatin (NAMPT) (NM_005746) Human Untagged Clone
CAT＃: SC111136
NAMPT (untagged)-Human nicotinamide phosphoribosyltransferase (NAMPT)
CNY 3,656.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110035O14Rik; PBEF; PBEF1; VF; VISFATIN |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111136 sequence for NM_005746 edited (data generated by NextGen Sequencing)
ATGAATCCTGCGGCAGAAGCCGAGTTCAACATCCTCCTGGCCACCGACTCCTACAAGGTT ACTCACTATAAACAATATCCACCCAACACAAGCAAAGTTTATTCCTACTTTGAATGCCGT GAAAAGAAGACAGAAAACTCCAAATTAAGGAAGGTGAAATATGAGGAAACAGTATTTTAT GGGTTGCAGTACATTCTTAATAAGTACTTAAAAGGTAAAGTAGTAACCAAAGAGAAAATC CAGGAAGCCAAAGATGTCTACAAAGAACATTTCCAAGATGATGTCTTTAATGAAAAGGGA TGGAACTACATTCTTGAGAAGTATGATGGGCATCTTCCAATAGAAATAAAAGCTGTTCCT GAGGGCTTTGTCATTCCCAGAGGAAATGTTCTCTTCACGGTGGAAAACACAGATCCAGAG TGTTACTGGCTTACAAATTGGATTGAGACTATTCTTGTTCAGTCCTGGTATCCAATCACA GTGGCCACAAATTCTAGAGAGCAGAAGAAAATATTGGCCAAATATTTGTTAGAAACTTCT GGTAACTTAGATGGTCTGGAATACAAGTTACATGATTTTGGCTACAGAGGAGTCTCTTCC CAAGAGACTGCTGGCATAGGAGCATCTGCTCACTTGGTTAACTTCAAAGGAACAGATACA GTAGCAGGACTTGCTCTAATTAAAAAATATTATGGAACGAAAGATCCTGTTCCAGGCTAT TCTGTTCCAGCAGCAGAACACAGTACCATAACAGCTTGGGGGAAAGACCATGAAAAAGAT GCTTTTGAACATATTGTAACACAGTTTTCATCAGTGCCTGTATCTGTGGTCAGCGATAGC TATGACATTTATAATGCGTGTGAGAAAATATGGGGTGAAGATCTAAGACATTTAATAGTA TCGAGAAGTACACAGGCACCACTAATAATCAGACCTGATTCTGGAAACCCTCTTGACACT GTGTTAAAGGTTTTGGAGATTTTAGGTAAGAAGTTTCCTGTTACTGAGAACTCAAAGGGT TACAAGTTGCTGCCACCTTATCTTAGAGTTATTCAAGGGGATGGAGTAGATATTAATACC TTACAAGAGATTGTAGAAGGCATGAAACAAAAAATGTGGAGTATTGAAAATATTGCCTTC GGTTCTGGTGGAGGTTTGCTACAGAAGTTGACAAGAGATCTCTTGAATTGTTCCTTCAAG TGTAGCTATGTTGTAACTAATGGCCTTGGGATTAACGTCTTCAAGGACCCAGTTGCTGAT CCCAACAAAAGGTCCAAAAAGGGCCGATTATCTTTACATAGGACGCCAGCAGGGAATTTT GTTACACTGGAGGAAGGAAAAGGAGACCTTGAGGAATATGGTCAGGATCTTCTCCATACT GTCTTCAAGAATGGCAAGGTGACAAAAAGCTATTCATTTGATGAAATAAGAAAAAATGCA CAGCTGAATATTGAACTGGAAGCAGCACATCATTAG Clone variation with respect to NM_005746.2 903 a=>g >OriGene 5' read for NM_005746 unedited
TATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTGAAGCTCATTTTTCTCCTT CCTCGCAGCCGCGCCAGGGAGCTCGCGGCGCGCGGCTCCTGTCCTCCGGCCCGAGATGAA TCCTGCGGCAGAAGCCGAGTTCAACATCCTCCTGGCCACCGACTCCTACAAGGTTACTCA CTATAAACAATATCCACCCAACACAAGCAAAGTTTATTCCTACTTTGAATGCCGTGAAAA GAAGACAGAAAACTCCAAATTAAGGAAGGTGAAATATGAGGAAACAGTATTTTATGGGTT GCAGTACATTCTTAATAAGTACTTAAAAGGTAAAGTAGTAACCAAAGAGAAAATCCAGGA AGCCAAAGATGTCTACAAAGAACATTTCCAAGATGATGTCTTTAATGAAAAGGGATGGAA CTACATTCTTGAGAAGTATGATGGGCATCTTCCAATAGAAATAAAAGCTGTTCCTGAGGG CTTTGTCATTCCCAGAGGAAATGTTCTCTTCACGGTGGAAAACACAGATCCAGAGTGTTA CTGGCTTACAAATTGGATTGAGACTATTCTTGTTCAGTCCTGGTATCCAATCACAGTGGC CACANATTCTAGAGAGCAGAGAAAATATTGGCCAAATATTTGTTAGAAACTTTCTGGGTA CTTAGATGGTCTGGAATACAANGTACATGAATTNTGGCTACAGAAGGAGTCTCTTNCCAA GAGACTGCTGGCATAAGAGCATCTGCTCACTTGGGTAAACTTCAAGGAACCGATACAGTA CCAGGGACTTGCTCTTATTAAAAAATATTATGGACGAAAGGATCCTGTTCCAGGCTTATT TTGTTCCACAGCAGAACCCAGTNCCTTTACAGCTTGGGGGGAAGACATGAANAAAATGCT TTGACATATTGAACCAAGTTTTATAAGGCCTGGTATTTGGGGCACGAA >OriGene 3' read for NM_005746 unedited
GGGCTTAGCTTGCCCGCGGCCGCTTTCTANGATCGAGTTTTTTTTTTTTTTTTTTGTTAA GGCCATGTTTTATTTGCTGATTAATGGACAAAAGGCAATGTAATTTATTTTCAAGTATTT TCTTGAAAGTCTGTGCTCATAAAAATCATGAAAAGTTGGAAAGACTGTTAAATCACTGAA ACTTCAAATATATCTTACACAATCTTGTTTGTACAAAAATACAAGTTAAATATAAACATA AAGCAATCATGGTAATTTTATGCAAATCTGTTTTATGTGATCATCAGTTATATATAAAAG TTTCTCAGTTCTGTTATTTGTGAAAAGATCAATACCAGATTGAATGACTACCTATTGGCA AAGGGCCCTAAAAAGCTTACTTTAGCACTCATCTTTTACATGGTTAAATGCATTTCCTAA TTTGAGATCACCTAAACACTGGAAAAGAAAAAAAATGAAAGGGCAGTATGTCCATAAACC AACAAATAATTTGGCTGTAATGTATCATAAAACACAAACCCCACACATCTGTACAATAAA CATTATGTATTACATACACACAACACACACCCAGTCATAAAGCCTAATGATGTGCTGCTT CCAGTTCAATATTCAGCTGTGCATTTTTTCTTATTTCATCAAATGAATAGCTTTTTGTCA CCTTGCCATTCTTGAAGACAGTATGGAGAAGATCCTGACCATATTCCTCAAGGTCTCCTT TTCCTTCTCCAGNGTAACAAAATTCCCTGCTGCGTCCTATGTAAAGATAATCGGCCCTTT TTGGACCTTTTGTTGGGATCAGCAACTGGGTTCTTGAAGACGTTAATCCCCAGGCCATTA GTTACCACATAGCTACACTTTGAAGAACCATTCAAGAGACTTTTGCCACTTCTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005746 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005746.1, NP_005737.1 |
| RefSeq Size | 2376 bp |
| RefSeq ORF | 1476 bp |
| Locus ID | 10135 |
| UniProt ID | P43490 |
| Domains | NAPRTase |
| Protein Families | Druggable Genome |
| Protein Pathways | Nicotinate and nicotinamide metabolism |
| Gene Summary | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
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Intracellular Nicotinamide Phosphoribosyltransferase Protects against Hepatocyte Apoptosis and Is Down-Regulated in Nonalcoholic Fatty Liver Disease
,Tuva B. Dahl, John Willy Haukeland, Arne Yndestad, Trine Ranheim, Ivar P. Gladhaug, Jan K. Damås, Terese Haaland, Else Marit Løberg, Borghild Arntsen, Kåre Birkeland, Kristian Bjøro, Stine M. Ulven, Zbigniew Konopski, Hilde I. Nebb, Pål Aukrust, and Bente Halvorsen,
J. Clin. Endocrinol. Metab., Jun 2010; 95: 3039 - 3047
[NAMPT]
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The Small Molecule GMX1778 is a Potent Inhibitor of NAD+ Biosynthesis: Strategy for Enhanced Therapy in NAPRT1-Deficient Tumors
,Mark Watson, Anne Roulston, Laurent Bélec, Xavier Billot, Richard Marcellus, Dominique Bédard, Cynthia Bernier, Stéphane Branchaud, Helen Chan, Kenza Dairi, Karine Gilbert, Daniel Goulet, Michel-Olivier Gratton, Henady Isakau, Anne Jang, Abdelkrim Khadir, Elizabeth Koch, Manon Lavoie, Michael Lawless, Mai Nguyen, Denis Paquette, Émilie Turcotte, Alvin Berger, Mathew Mitchell, Gordon C. Shore, and Pierre Beauparlant,
Mol. Cell. Biol., Aug 2009; 10.1128/MCB.00112-09
[NAMPT]
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Visfatin activates eNOS via Akt and MAP kinases and improves endothelial cell function and angiogenesis in vitro and in vivo: translational implications for atherosclerosis
,Fina Lovren, Yi Pan, Praphulla C. Shukla, Adrian Quan, Hwee Teoh, Paul E. Szmitko, Mark D. Peterson, Milan Gupta, Mohammed Al-Omran, and Subodh Verma,
Am J Physiol Endocrinol Metab, Jun 2009; 296: E1440 - E1449.
[NAMPT]
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Visfatin (nicotinomide phosphoribosyltransferase/pre-B cell colony-enhancing factor) activates eNOS via Akt and MAP kinases and improves endothelial function
,Fina Lovren, Yi Pan, Praphulla C Shukla, Adrian Quan, Hwee Teoh, Paul Edward Szmitko, Mark D Peterson, Mohammed Al-Omran, and Subodh Verma,
Am J Physiol Endocrinol Metab, Apr 2009; 10.1152/ajpendo.90780.2008.
[NAMPT]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210707 | NAMPT (Myc-DDK-tagged)-Human nicotinamide phosphoribosyltransferase (NAMPT) |
CNY 3,656.00 |
|
| RC210707L1 | Lenti-ORF clone of NAMPT (Myc-DDK-tagged)-Human nicotinamide phosphoribosyltransferase (NAMPT) |
CNY 6,056.00 |
|
| RC210707L2 | Lenti-ORF clone of NAMPT (mGFP-tagged)-Human nicotinamide phosphoribosyltransferase (NAMPT) |
CNY 5,890.00 |
|
| RC210707L3 | Lenti-ORF clone of NAMPT (Myc-DDK-tagged)-Human nicotinamide phosphoribosyltransferase (NAMPT) |
CNY 6,056.00 |
|
| RC210707L4 | Lenti-ORF clone of NAMPT (mGFP-tagged)-Human nicotinamide phosphoribosyltransferase (NAMPT) |
CNY 6,056.00 |
|
| RG210707 | NAMPT (tGFP-tagged) - Human nicotinamide phosphoribosyltransferase (NAMPT) |
CNY 5,256.00 |
|
| SC322049 | NAMPT (untagged)-Human nicotinamide phosphoribosyltransferase (NAMPT) |
CNY 3,656.00 |
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