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GDA (NM_004293) Human Untagged Clone
CAT＃: SC111122
GDA (untagged)-Human guanine deaminase (GDA)
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CYPIN; GAH; GUANASE; NEDASIN |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111122 sequence for NM_004293 edited (data generated by NextGen Sequencing)
ATGTGTGCCGCTCAGATGCCGCCCCTGGCGCACATCTTCCGAGGGACGTTCGTCCACTCC ACCTGGACCTGCCCCATGGAGGTGCTGCGGGATCACCTCCTCGGCGTGAGCGACAGCGGC AAAATAGTGTTTTTAGAAGAAGCATCTCAACAGGAAAAACTGGCCAAAGAATGGTGCTTC AAGCCGTGTGAAATAAGAGAACTGAGCCACCATGAGTTCTTCATGCCTGGGCTGGTTGAT ACACACATCCATGCCTCTCAGTATTCCTTTGCTGGAAGTAGCATAGACCTGCCACTCTTG GAGTGGCTGACCAAGTACACATTTCCTGCAGAACACAGATTCCAGAACATCGACTTTGCA GAAGAAGTATATACCAGAGTTGTCAGGAGAACACTAAAGAATGGAACAACCACAGCTTGT TACTTTGCAACAATTCACACTGACTCATCTCTGCTCCTTGCCGACATTACAGATAAATTT GGACAGCGGGCATTTGTGGGCAAAGTTTGCATGGATTTGAATGACACTTTTCCAGAATAC AAGGAGACCACTGAGGAATCGATCAAGGAAACTGAGAGATTTGTGTCAGAAATGCTCCAA AAGAACTATTCTAGAGTGAAGCCCATAGTGACACCACGTTTTTCCCTCTCCTGCTCTGAG ACTTTGATGGGTGAACTGGGCAACATTGCTAAAACCCGTGATTTGCACATTCAGAGCCAT ATAAGTGAAAATCGTGATGAAGTTGAAGCTGTGAAAAACTTATACCCCAGTTATAAAAAC TACACATCTGTGTATGATAAAAACAATCTTTTGACAAATAAGACAGTGATGGCACACGGC TGCTACCTCTCTGCAGAAGAACTGAACGTATTCCATGAACGAGGAGCATCCATCGCACAC TGTCCCAATTCTAATTTATCGCTCAGCAGTGGATTTCTAAATGTGCTAGAAGTCCTGAAA CATGAAGTCAAGATAGGGCTGGGTACAGACGTGGCTGGTGGCTATTCATATTCCATGCTT GATGCAATCAGAAGAGCAGTGATGGTTTCCAATATCCTTTTAATTAATAAGGTAAATGAG AAAAGCCTCACCCTCAAAGAAGTCTTCAGACTAGCTACTCTTGGAGGAAGCCAAGCCCTG GGGCTGGATGGTGAGATTGGAAACTTTGAAGTGGGCAAGGAATTTGATGCCATCCTGATC AACCCCAAAGCATCCGACTCTCCCATTGACCTGTTTTATGGGGACTTTTTTGGTGATATT TCTGAGGCTGTTATCCAGAAGTTCCTCTATCTAGGAGATGATCGAAATATTGAAGAGGTT TATGTGGGCGGAAAGCAGGTGGTTCCGTTTTCCAGCTCAGTGTAA Clone variation with respect to NM_004293.3 >OriGene 5' read for NM_004293 unedited
GGCGGCCCGCGAATTCGCACGAGGCAGCCCCTGGGCGCGGCCTGCAGGGTACCGGCAACC GCCCGGGTAAGCGGGGGCAGGACAAGGCCGGAGCCTGTGTCCGCCCGGCAGCCGCCCGCA GCTGCAGAGAGTCCCGCTGCGTCTCCGCCGCGTGCGCCCTCCTCGACCAGCAGACCCGCG CTGCGCTCCGCCGCTGACATGTGTGCCGCTCAGATGCCGCCCCTGGCGCACATCTTCCGA GGGACGTTCGTCCACTCCACCTGGACCTGCCCCATGGAGGTGCTGCGGGATCACCTCCTC GGCGTGAGCGACAGCGGCAAAATAGTGTTTTTAGAAGAAGCATCTCAACAGGAAAAACTG GCCAAAGAATGGTGCTTCAAGCCGTGTGAAATAAGAGAACTGAGCCACCATGAGTTCTTC ATGCCTGGGCTGGTTGATACACACATCCATGCCTCTCAGTATTCCTTTGCTGGAAGTAGC ATAGACCTGCCACTCTTGGAGTGGCTGACCAAGTACACATTTCCTGCAGAACACAGATTC CAGAACATCGACTTTGCAGAAGAAGTATATCCCAGAGTTGTCAGGAGAACACTAAAGAAT GGAACAACCACAGCTTGTTACTTTGCAACAATTCACACTGACTCATCTCTGCTCCTTGCC GACATTACAGATAAATTTGGACAGCGGGCATTTGTGGGCANAGTTTGCATGGATTTGAAT GACACTTTTCCAGAATACANGGAGACCACTGGAGGAATCGATCAAGGAAACTGAGAGATT TGTGTCAGAAATGCTNNCAAAGAACTATTCTAGAGTGAAAGCCATAGTGACACCACGTTN TTCCCTCTCCTGCNTCTGAGACTTGATGGGTGAACTGGGGCACATTGCCTAAACCCGNTG ATTTGCACATTCAGAGCCATATAGGTGAAAATCGTGATGAG >OriGene 3' read for NM_004293 unedited
GAAATCCTATGNNACCGCGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTT TTAAACACAAAGTCTCATTTATTTTTGTCTGAAGCACACAGGAGCTCACTCAGCACAATA ACAGTAAGCGAATCATACAAATATTGAGAAAAAATGTTCCTATGAATACATACATGTATA TTCTTAAGAGTAGCGATCAGGAGTTTAACAACAAATGTAAAGTGGTTTTCTCTAAAGAAT GCTTTCTGACAGGCTTTTGGGTTGGAAATGGACAGGTAAATCACTGTCACATAACAGGTA AGCTAAGAATAACTTCTGTTACCCAAGTCATTTGAACCCTGTGGACTGTGAAAGCCCTCT TGGAATTTACATTTAATTCCATCATTGGTCTGGTTGACTTCCACATTTCACTAAATTTGG ACAAGATCCACAAAGTAACTCCTCAACTCTCAGTCTTTCACACTCAGGTCTGTGGGAAGG AAAGGCAGTGAGACCAGGTGTGAACACATGCACGGAGACCAATGTTAGGATTTTCTAACA GTATCCTTCTGTTTCTGCACTTCCAAAGTCATACCTAAACTGGCTAGTATATCTGAAATT GCTAACATCTCCTTGAAGCTGTCCAAAAAAAAAAATTTTTTTTCAACATTCTTAACATCC AACAATTTAAAACCTTTCAAGTCTTGCCTANACACTTCTAATTTACCATTATAACTCACA CCATTGCTTTTAAGCGTGTGAAATGTTCCAGCAATCCTCAACTTTTAAAATGGACAACAA TGGAAAGATGTCTAAAAAGTCTTCCGAAGCATCCCAATTTTGGGATCTCTTCCTACGTTA GCCCAAGGTTGAAATTGATACAATGGGGCCTTCCAACTTGGCCTTTTAAGCTCTCCCAAT GTCTAAGTTTGGAGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004293 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004293.2, NP_004284.1 |
| RefSeq Size | 5451 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 9615 |
| UniProt ID | Q9Y2T3 |
| Domains | Amidohydro_1 |
| Protein Pathways | Metabolic pathways, Purine metabolism |
| Gene Summary | This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the C-terminus compared to isoform a. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Alteration of enzyme specificity by computational loop remodeling and design
,Paul M. Murphy, Jill M. Bolduc, Jasmine L. Gallaher, Barry L. Stoddard, and David Baker,
PNAS, Jun 2009; 106: 9215 - 9220.
[GDA]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208771 | GDA (Myc-DDK-tagged)-Human guanine deaminase (GDA) |
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| RC208771L1 | Lenti ORF clone of Human guanine deaminase (GDA), Myc-DDK-tagged |
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| RC208771L2 | Lenti ORF clone of Human guanine deaminase (GDA), mGFP tagged |
CNY 5,890.00 |
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| RC208771L3 | Lenti ORF clone of Human guanine deaminase (GDA), Myc-DDK-tagged |
CNY 5,890.00 |
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| RC208771L4 | Lenti ORF clone of Human guanine deaminase (GDA), mGFP tagged |
CNY 5,890.00 |
|
| RG208771 | GDA (tGFP-tagged) - Human guanine deaminase (GDA) |
CNY 5,256.00 |
