PIGL (NM_004278) Human Untagged Clone

CAT#: SC111117

PIGL (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class L (PIGL)



  "NM_004278" in other vectors (4)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-PIGL Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CHIME
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004278, the custom clone sequence may differ by one or more nucleotides


ATGGAAGCAATGTGGCTCCTGTGTGTGGCGTTGGCGGTCTTGGCATGGGGCTTCCTCTGGGTTTGGGACT
CCTCAGAACGAATGAAGAGTCGGGAGCAGGGAGGACGGCTGGGAGCCGAAAGCCGGACCCTGCTGGTCAT
AGCGCACCCTGACGATGAAGCCATGTTTTTTGCTCCCACAGTGCTAGGCTTGGCCCGCCTAAGGCACTGG
GTGTACCTGCTTTGCTTCTCTGCAGGAAATTACTACAATCAAGGAGAGACTCGTAAGAAAGAACTTTTGC
AGAGCTGTGATGTTTTGGGGATTCCACTCTCCAGTGTAATGATTATTGACAACAGGGATTTCCCAGATGA
CCCAGGCATGCAGTGGGACACAGAGCACGTGGCCAGAGTCCTCCTTCAGCACATAGAAGTGAATGGCATC
AATCTGGTGGTGACTTTCGATGCAGGGGGAGTAAGTGGCCACAGCAATCACATTGCTCTGTATGCAGCTG
TGAGGGCCCTGCACTCAGAAGGGAAGTTACCTAAAGGGTGCTCTGTGCTCACGCTTCAGTCTGTGAATGT
GCTGCGCAAGTACATCTCCCTTCTGGATCTGCCCTTGTCTCTGCTTCATACGCAGGATGTCCTCTTCGTG
CTCAACAGCAAAGAAGTGGCACAGGCCAAGAAAGCCATGTCCTGCCACCGCAGCCAGCTCCTCTGGTTCC
GCCGCCTCTACATTATCTTCTCCCGGTACATGAGAATCAACTCACTGAGCTTCCTCTGA


>OriGene 5' read for NM_004278 unedited
TTCAGATTTGTATCCGACTCACTATAGGGCGGCCGCGCATTCGGCACGAGGCAATGTGGC
TCCTGTGTGTGGCGTTGGCGGTCTTGGCATGGGGCTTCCTCTGGGTTTGGGACTCCTCAG
AACGAATGAAGAGTCGGGAGCAGGGAGGACGGCTGGGAGCCGAAAGCCGGACCCTGCTGG
TCATAGCGCACCCTGACGATGAAGCCATGTTTTTTGCTCCCACAGTGCTAGGCTTGGCCC
GCCTAAGGCACTGGGTGTACCTGCTTTGCTTCTCTGCAGGAAATTACTACAATCAAGGAG
AGACTCGTAAGAAAGAACTTTTGCAGAGCTGTGATGTTTTGGGGATTCCACTCTCCAGTG
TAATGATTATTGACAACAGGGATTTCCCAGATGACCCAGGCATGCAGTGGGACACAGAGC
ACGTGGCCAGAGTCCTCCTTCAGCACATAGAAGTGAATGGCATCAATCTGGTGGTGACTT
TCGATGCAGGGGGAGTAAGTGGCCACAGCAATCACATTGCTCTGTATGCAGCTGTGAGGG
CCCTGCACTCAGAAGGGAAGTTACCTAAAGGTAAGGCTTGTTCCTTTTGCAAAGGGCCAC
AAGATACTGTCCCTCTGAGAAACTTATGAGGGGGAAATTTGCAAATCCACAGAGAGCTGC
CCTCAGCCGAGCAGAGGCAGTGGTTGGGGAGGCCGNCACACTCTTTCTGACTCACCTCAA
GGTACCTGGCAGGTTGTATCTACCAGCAAGTGCCTGCCTGCANGGCCACCTCTGGATGAG
TGGGGAGGCCACGTTTGCCCCTCACCTGTCTAGCTTTTCACAGGGTGGATGTTTTACACC
TCTGGTATTCTCCAGTCTGAACTCAATCCCATTCCCCACCAATAAGCAGGTTGTAATGAC
TGCTGGGGGCTTGATGACCCATCTGC
>OriGene 3' read for NM_004278 unedited
ATGAACCGCGGCCCGCTTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTTTTAGAGCAC
TTTTATGTTCATTTATGATAATAGGGACCTGCCCAGGCACTGGGCTAGGTGTTCCACATC
TACTGTGTCATTATGTAAATTGTTTTTCACAAGAACTTGAGAAGCTAGCAGTGTAGCTAT
TATTATCCTTGGGGTGGTTTGTTTTTGGTTCTTTGGGTTTTTTCCCAGGGGAAGAAGCTG
GAGTTTGGACAGCCCCTGGCCTACATGGGCTCCCTTTTGCAGAGGCTGCTCCAGCGGGGA
TCTCCTTGAGCTCAGGTAAATAAGCCAGTGCCAGGCCAGGTCCTCTGCACTCCTTTGTCT
ATTTTCCCCTTTGTTCCTTGGACCTGAAAACCCTTCAAGGCTTCAGAGGAAGCTCAGTGA
GTTGATTCTCATGTACCGGGAGAAGATAATGCAGAGGCGGCGGAACCAGAGGAGCTGGCT
GCGGTGGCAGGACATGGCTTTCTTGGCCTGTGCCACTTCTTTGCTGTTGAGCACGAAGAG
GACATCCTGCGTATGAAGCAGAGACAAGGGCAGATCCAGAAGGGAGATGTACTTGCGCAG
CACATTCACAGACTGAAGCGTGAGCACAGAGCACCCTGGATGGAGAGGAGACAGGGTGAA
GCGATAAGCCTTGAGGCCTCCAGCCAGATTCTCCCCTGAGCATCCTCCCTCTACCCTGTG
GCTCAAGGCAGGGCAGGACAGTGGCCACAGCATTGCCCCGTGTTTCTGTGGCACTACAGC
TGCCNTGTGCCCATGATTCTTGCAGCTAGAACGAGTATAAGGGCCATAGAAGACCTGGCT
TGCTACCCTCTACCCCTGCGGTGTGCCCTACCANAAAAACGTTGTCTGCTTACAGCGTGA
AACTTTGCTTACCTCAAAGCCAAAGCAAGTAAG
Restriction Sites NotI-NotI     
ACCN NM_004278
Insert Size 2090 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004278.2, NP_004269.1
RefSeq Size 1163 bp
RefSeq ORF 759 bp
Locus ID 9487
UniProt ID Q9Y2B2
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
Gene Summary This gene encodes an enzyme that catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which is the de-N-acetylation of N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI). Study of a similar rat enzyme suggests that this protein localizes to the endoplasmic reticulum. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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