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N WASP (WASL) (NM_003941) Human Untagged Clone
CAT＃: SC111092
WASL (untagged)-Human Wiskott-Aldrich syndrome-like (WASL)
CNY 3,768.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | N-WASP; NWASP; WASPB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111092 sequence for NM_003941 edited (data generated by NextGen Sequencing)
ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCTCTG TTGCTCACCCCGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACT ATGTCTTCAGCAGTGGTGCAGTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAG TGCAGTGGTGTTGCTTGTCTTGTTAAGGACAATCCACAGAGATCTTATTTTTTAAGAATA TTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGCTATACAATAACTTTGTATAT AATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTTGCTCTTAAT TTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCT ACAGTTGATATAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAAC AACATCTCCCATACCAAAGAAAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAG GCAGATATAGGAACACCAAGCAATTTCCAGCACATTGGACATGTTGGTTGGGATCCAAAT ACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGAATCTTTTCGATATGTGTGGA ATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGACTTTATTGAA AAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCT CCTCCTCCTGCTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCT GCACCTCCACCACCGCCTCCTTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAAT AGGATGTACCCTCCTCCACCTCCAGCCCTTCCCTCCTCAGCACCTTCAGGGCCTCCACCA CCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCACCCCCACCGCCTCCACCTCCA CCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCATCAGGTTCCA ACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAG ATACGACAGGGTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACA CCTGCACCCACTTCAGGAATTGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAA GCCATTCATTCTTCAGATGAAGATGAAGATGAAGATGATGAAGAAGATTTTGAGGATGAT GATGAGTGGGAAGACTGA Clone variation with respect to NM_003941.2 57 c=>t >OriGene 5' read for NM_003941 unedited
GGAAGCGTACAAATTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCC GCCGCTGCGGGAGGGTTGGGGGAGGAGTTGGGAGTTTAGCGCAGTCGCCGGAGTGCGAGG ACAACGACCATCCGGCCCTAGCCTGGCCGGGCGGGTGCCGGGAGCTTCCCTTTCTCAGCG CGGCGCGAAGGTGGCTCGCCGTCAGCGCCTGCTTCCCTCGACCTCGTCCTCCTCCCCGCT CCGGAGGAGCTGCGAGATGTGGCGCCTCTGACTCCACTTCTCCCCGCCCCTGTCACCGAG AGGGGGAACGAGCTCTCGCCCACTCGCCGGAGGAGACGGCCCTGGACTCCCAACCCCGCC GGCGAAACCATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTG GGGTCTCTGTTGCTCACCCCGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAA TGTGTGACTATGTCTTCAGCAGTGGTGCAGTTATATGCAGCAGATCGGAACTGTATGTGG TCAAAGAAGTGCAGTGGTGTTGCTTGTCTTGTTAAGGACAATCCACAGAGATCTTATTTT TTAAGAATATTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGCTATACAATAAC TTTGTATATAATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTT GCTCTTAATTTTGCCAATGAAGAAGAAGCANAAAAATTTCGAAAAGCAGTTACAGACCTT TTGGGCCGTCGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTA CCATGGCTACAGTTGATATAAAAAATCCAGAAATCACACANATAGATTT >OriGene 3' read for NM_003941 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGTCAAATTAATTTTATT ATGCTCCATGATGAATTGCCACCAGTGCAACATCCTATTCACTATACATTTCAAAAAAAG AATTCACATACTAAACAAAATTTCAGTTGTCTGAAAATGAAATGATTGAAAGTCTTTATG AATCTCATACATACAATATGTGGCTAGCTGAAATTGTCTATCACGTAGCATTTAGATATA AAAAGCCTCATGCTAGTTTGTTAAATGCAAAGGCTACCAGACGACCATTTAGCTGGAGAA TATACGGAAGGCTTTCAGACAACGCACAGGTATAGTGCTGCTCACAGTGCAGGATGGTAG AGGACTGAAACATGCAACCTTACACCTTACTTGGTAAAGCAGATTTAGTCTTCATGCCTG GACTGAACTCCACAGCTGCTGTGTTTCAACCAACAGTAATTTAGACTTTTTGTAACAACA ACCAATGTCTTTTTCTTCTTTAAGAAAAAAAGAATAAATTAATTACCTTTGGCCAATTAT ATATCCCTTTTACATGTTTAAAATTGTGCCTTTGAACTCGCAAAATGGGTACTCTCTGCT GAGAGAAGTTCCTGATAAATATATATTTATACCAAAGGCAGCTGGAAAATCTAGAATTTC TTTGCCACAAGTTAAGAGAGATCTTAAACTGGCACTTACCCTGGAGAACTTTCCTAGGAA ATCTAAAAAGGTTCTTCATCCCGGAAAAAACAAAAAAATTTTCCAATTTACCTTCACTCA AAAAAACTATAATGGGCGCTTTTAATTTTTGCCCAACCTTCTGCATTTATTGGCTAACAC CTGTCCAACTCCATTTGCCCAAATTCGCTGGGCCTCACCCCCCAAAAATGATGTCTTCCC AAAAGGGCTTCTCCACCGAGGGCCCGTTTTCCCCACAAACAAAAGCCACAATTTTCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003941 |
| Insert Size | 4540 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003941.2, NP_003932.3 |
| RefSeq Size | 4430 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 8976 |
| UniProt ID | O00401 |
| Domains | PBD, WH1, WH2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Role of N-WASP in Endothelial Monolayer Formation and Integrity
,Mooren, OL;Kim, J;Li, J;Cooper, JA;,
J. Biol. Chem.
,PubMed ID 26070569
[WASL]
|
|
Novel Role of Rac1/WAVE Signaling Mechanism in Regulation of the Epithelial Na+ Channel
,Alexey V. Karpushev, Vladislav Levchenko, Daria V. Ilatovskaya, Tengis S. Pavlov, and Alexander Staruschenko,
Hypertension, May 2011; 57: 996 - 1002
[WASL]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207967 | WASL (Myc-DDK-tagged)-Human Wiskott-Aldrich syndrome-like (WASL) |
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|
| RC207967L1 | Lenti ORF clone of Human Wiskott-Aldrich syndrome-like (WASL), Myc-DDK-tagged |
CNY 6,160.00 |
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| RC207967L2 | Lenti ORF clone of Human Wiskott-Aldrich syndrome-like (WASL), mGFP tagged |
CNY 6,460.00 |
|
| RC207967L3 | Lenti ORF clone of Human Wiskott-Aldrich syndrome-like (WASL), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207967L4 | Lenti ORF clone of Human Wiskott-Aldrich syndrome-like (WASL), mGFP tagged |
CNY 6,460.00 |
|
| RG207967 | WASL (tGFP-tagged) - Human Wiskott-Aldrich syndrome-like (WASL) |
CNY 5,360.00 |
