United States
Germany
Japan
United Kingdom
China
DGKE (NM_003647) Human Untagged Clone
CAT＃: SC111075
DGKE (untagged)-Human diacylglycerol kinase, epsilon 64kDa (DGKE)
CNY 4,232.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AHUS7; DAGK5; DAGK6; DGK; NPHS7 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_003647 edited
GCGTCGTTCTCCTCCTGCGCGAGGCGGCCAAGGCCTGCTGGCCCGGAGCCGCGCCTCCAC CCGCGCGAGGTATCGTCCTTGGAGAAGATGGAAGCGGAGAGGCGGCCGGCGCCGGGCTCG CCCTCCGAGGGCCTGTTTGCGGACGGGCACCTGATCTTGTGGACGCTGTGCTCGGTCCTG CTGCCGGTGTTCATCACCTTCTGGTGTAGCCTCCAGCGGTCGCGCCGGCAGCTGCACCGC AGGGACATCTTCCGCAAGAGCAAGCACGGATGGCGCGACACGGACCTGTTCAGCCAGCCC ACCTACTGCTGCGTGTGCGCGCAGCACATTCTGCAGGGCGCCTTCTGCGACTGCTGCGGG CTCCGCGTGGACGAGGGCTGCCTCAGGAAGGCCGACAAGCGCTTCCAGTGCAAGGAGATT ATGCTCAAGAATGACACCAAGGTCCTGGACGCCATGCCCCACCACTGGATCCGGGGCAAC GTGCCGCTGTGCAGTTACTGTATGGTTTGCAAGCAGCAGTGTGGCTGTCAACCCAAGCTT TGCGATTACAGGTGCATTTGGTGCCAGAAAACAGTACATGATGAGTGCATGAAAAATAGT TTAAAGAATGAAAAATGTGATTTTGGAGAATTCAAAAACCTAATCATTCCACCAAGTTAT TTAACATCCATTAATCAGATGCGTAAAGACAAAAAAACAGATTATGAAGTGCTAGCCTCT AAGCTTGGAAAGCAGTGGACCCCATTAATAATCCTGGCCAACTCTCGTAGTGGAACTAAT ATGGGAGAAGGACTGTTGGGAGAATTTAGGATCTTGTTGAATCCAGTCCAGGTTTTTGAT GTAACTAAAACTCCTCCTATCAAAGCCCTACAACTCTGTACTCTTCTCCCATATTATTCA GCTCGAGTACTTGTTTGTGGAGGGGATGGGACTGTAGGGTGGGTCCTGGATGCAGTTGAT GACATGAAGATTAAGGGACAAGAAAAGTACATTCCACAAGTTGCAGTTTTGCCTCTGGGA ACAGGCAACGATCTATCCAATACATTGGGTTGGGGTACAGGTTATGCTGGAGAAATTCCA GTTGCGCAGGTTTTGCGAAATGTAATGGAAGCAGATGGAATTAAACTAGATCGATGGAAA GTTCAAGTAACAAATAAAGGATACTACAACTTAAGAAAACCCAAGGAATTCACAATGAAC AACTATTTTTCTGTTGGACCTGATGCTCTCATGGCTCTCAATTTTCATGCTCATCGTGAG AAGGCACCATCTCTGTTTTCTAGCAGAATTCTTAATAAGGCGGTTTACTTATTCTATGGA ACCAAAGATTGTTTAGTGCAAGAATGTAAAGATTTGAATAAAAAAGTTGAGCTAGAACTG GATGGTGAGCGAGTAGCACTGCCCAGCTTGGAAGGTATTATAGTTCTGAACATCGGATAC TGGGGCGGTGGCTGCAGACTATGGGAAGGGATGGGGGACGAGACTTACCCTCTAGCCAGG CATGACGATGGTCTGCTGGAAGTCGTTGGAGTATATGGGTCTTTCCACTGTGCTCAGATT CAAGTAAAACTGGCTAATCCTTTTCGAATAGGACAGGCACATACAGTGAGGCTGATTTTG AAGTGCTCCATGATGCCAATGCAGGTGGATGGGGAGCCTTGGGCCCAAGGGCCCTGCACT GTCACCATAACTCACAAGACACATGCAATGATGTTATATTTCTCTGGAGAACAAACAGAT GATGACATCTCTAGTACTTCGGATCAAGAAGATATAAAGGCGACTGAATAGATGGATGAG GGAGTGAAAACTTTGCATAGAATCCTCACGCAAGTAGATACATGTTCATCCAAAAGTATT AATAGAAATTCTCTATCAGCTATTCAGTCTT >OriGene 5' read for NM_003647 unedited
TTATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC GCGAATTCGGCACGAGGGCTTCATCCCTGCTGGCGGCCCAGCGTCGTTCTCCTCCTGCGC GAGGCGGCCAAGGCCTGCTGGCCCGGAGCCGCGCCTCCACCCGCGCGAGGTATCGTCCTT GGAGAAGATGGAAGCGGAGAGGCGGCCGGCGCCGGGCTCGCCCTCCGAGGGCCTGTTTGC GGACGGGCACCTGATCTTGTGGACGCTGTGCTCGGTCCTGCTGCCGGTGTTCATCACCTT CTGGTGTAGCCTCCAGCGGTCGCGCCGGCAGCTGCACCGCAGGGACATCTTCCGCAAGAG CAAGCACGGATGGCGCGACACGGACCTGTTCAGCCAGCCCACCTACTGCTGCGTGTGCGC GCAGCACATTCTGCAGGGCGCCTTCTGCGACTGCTGCGGGCTCCGCGTGGACGAGGGCTG CCTCAGGAAGGCCGACAAGCGCTTCCAGTGCAAGGAGATTATGCTCAAGAATGACACCAA GGTCCTGGACGCCATGCCCCACCACTGGATCCGGNGCAACGTGCCGCTGTGCAGTTACTG TATGGTTTGCAAGCAGCAGTGTGGCTGTCAACCCAAGCTTTGCGATTACAGGTGCATTTG GNTGCCAGAAACAGTACATGATGAGTGCATGANANATAGTTAANAGAAATGAAAATGTGA TTTTGGNAGAAATCANAACCCTATCATTCCACCAGTTATTAACATCCATTATCAGATGCG NTAAGACAAAAAACAGATTATGAAGTGCTAGCCTCTAAGCTGGAAAGCAGTGGACCCCAT TNATATNCTGGNCACTCTCGTAGTGGACTATATGGGAGAA >OriGene 3' read for NM_003647 unedited
NNNNNNCCGGTTTAGCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTT TTTTTAAATATCTAGATATTTTAAATATATCTACTTATATTCCACTGTCCTCAACTGGGT AGAAATATTAAAAAAATTATCAATATAAAATGCTGGTTCTTGCTTCAATATTATCATTTT GAAGGAAAACCACACAAAACCATTACCCATTCTAATGACTTTTTTGGCCAAATTATGTAT TTGGGTCCACATTAATCATTAAATTAGTATAACCTTTAAAGTTTTCAAGTTAGTGCAAAT TTAAAATTCAATAAATATATTAAATACAATTTTACTTTATATTACAAATGTAAATTATTT TTCTTTTGACATGCTGGTAGTATTCATTTTTACAACGCAAACTGACTTTACAATTTCTGA GTCACAGTATATTTTATAACTCTAAGCAAATGTATTTCACAAAGCAAACACTATATGTTT TTGACATACTCTAAAAAAATAAAAATAAATAATTTTGATTATAAAATTTGTATAAACTTA GCTACATTAGGGGTTCACAGCTAATTGTTGCAGAAAACCTAAAACACACCTAAATCATGT AACTTTACTAGCAGACAACTTGTATTTTAAAACACAAGTCAGTTTGATTTCTATTAAACA TGACGAAACACTGAAAATTATCATCATCTGCCTAAGGGATCTTTAAGAGCTGCCAGGTAT TCAGTATTTAAGCCACTACGTTTATAGGAATTAGGAATGTATTGGGATAGTAAAAAGATT AGAGAAATCTGGAAACAGACATGGAGATATGATTTACTTCATGTATTTCTTTCCTGAGGA TGTAGAAGTTATTTTTTTTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003647 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003647.1, NP_003638.1 |
| RefSeq Size | 2562 bp |
| RefSeq ORF | 1704 bp |
| Locus ID | 8526 |
| UniProt ID | P52429 |
| Domains | DAGKa, DAGKc, DAG_PE-bind |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219913 | DGKE (Myc-DDK-tagged)-Human diacylglycerol kinase, epsilon 64kDa (DGKE) |
CNY 4,224.00 |
|
| RC219913L1 | Lenti ORF clone of Human diacylglycerol kinase, epsilon 64kDa (DGKE), Myc-DDK-tagged |
CNY 6,624.00 |
|
| RC219913L2 | Lenti ORF clone of Human diacylglycerol kinase, epsilon 64kDa (DGKE), mGFP tagged |
CNY 5,890.00 |
|
| RC219913L3 | Lenti ORF clone of Human diacylglycerol kinase, epsilon 64kDa (DGKE), Myc-DDK-tagged |
CNY 6,624.00 |
|
| RC219913L4 | Lenti ORF clone of Human diacylglycerol kinase, epsilon 64kDa (DGKE), mGFP tagged |
CNY 5,890.00 |
|
| RG219913 | DGKE (tGFP-tagged) - Human diacylglycerol kinase, epsilon 64kDa (DGKE) |
CNY 4,370.00 |
