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Triosephosphate isomerase (TPI1) (NM_000365) Human Untagged Clone
CAT＃: SC111041
TPI1 (untagged)-Human triosephosphate isomerase 1 (TPI1), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-S-49; TIM; TPI; TPID |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111041 sequence for NM_000365 edited (data generated by NextGen Sequencing)
ATGGCGCCCTCCAGGAAGTTCTTCGTTGGGGGAAACTGGAAGATGAACGGGCGGAAGCAG AGTCTGGGGGAGCTCATCGGCACTCTGAACGCGGCCAAGGTGCCGGCCGACACCGAGGTG GTTTGTGCTCCCCCTACTGCCTATATCGACTTCGCCCGGCAGAAGCTAGATCCCAAGATT GCTGTGGCTGCGCAGAACTGCTACAAAGTGACTAATGGGGCTTTTACTGGGGAGATCAGC CCTGGCATGATCAAAGACTGCGGAGCCACGTGGGTGGTCCTGGGGCACTCAGAGAGAAGG CATGTCTTTGGGGAGTCAGATGAGCTGATTGGGCAGAAAGTGGCCCATGCTCTGGCAGAG GGACTCGGAGTAATCGCCTGCATTGGGGAGAAGCTAGATGAAAGGGAAGCTGGCATCACT GAGAAGGTTGTTTTCGAGCAGACAAAGGTCATCGCAGATAACGTGAAGGACTGGAGCAAG GTCGTCCTGGCCTATGAGCCTGTGTGGGCCATTGGTACTGGCAAGACTGCAACACCCCAA CAGGCCCAGGAAGTACACGAGAAGCTCCGAGGATGGCTGAAGTCCAACGTCTCTGATGCG GTGGCTCAGAGCACCCGTATCATTTATGGAGGCTCTGTGACTGGGGCAACCTGCAAGGAG CTGGCCAGCCAGCCTGATGTGGATGGCTTCCTTGTGGGTGGTGCTTCCCTCAAGCCCGAA TTCGTGGACATCATCAATGCCAAACAATGA Clone variation with respect to NM_000365.5 >OriGene 5' read for NM_000365 unedited
GTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGTCGGCTCCGC GCCTGGCGCCCTCCAGGAATTCTTCGTTGGGGGAAACTGGAAGATGAACGGGCGGAAGCA GAGTCTGGGGGAGCTCATCGGCACTCTGAACGCGGCCAAGGTGCCGGCCGACACCGAGGT GGTTTGTGCTCCCCCTACTGCCTATATCGACTTCGCCCGGCAGAAGCTAGATCCCAAGAT TGCTGTGGCTGCGCAGAACTGCTACAAAGTGACTAATGGGGCTTTTACTGGGGAGATCAG CCCTGGCATGATCAAAGACTGCGGAGCCACGTGGGTGGTCCTGGGGCACTCAGAGAGAAG GCATGTCTTTGGGGAGTCAGATGAGCTGATTGGGCAGAAAGTGGCCCATGCTCTGGCAGA GGGACTCGGAGTAATCGCCTGCATTGGGGAGAAGCTAGATGAAAGGGAAGCTGGCATCAC TGAGAAGGTTGTTTTCGAGCAGACAAAGGTCATCGCAGATAACGTGAAGGACTGGAGCAA GGTCGTCCTGGCCTATGAGCCTGTGTGGGCCATTGGTACTGGCAAGACTGCAACACCCCA ACAGGCCCAGGAAGTACACGAGAAGCTCCGAGGATGGCTGAAGTCCAACGTCTCTGATGC GGTGGCTCAGAGCACCCGTATCATTTATNGGAGCTCTGTGACTGGGGCAACCTGCAGGGA GCTGGCCAGCCAGCCTGAGTTGGATGGCTTCCTTGTGGGGTGTTGCTTCCCTCAGCCCGA ATTCGTNGACATCATCAATGCCAAAACATGAGCCCATNCATCTTCCCTACCCTTCCTGCC CAGCCAGGGACTAAGCAGNCCAGAAGCCCAGTACTGCCCTTNCCTGCATATGCTCTGATG GGNCATCTGCTCCTTCTGG >OriGene 3' read for NM_000365 unedited
GTGGCAACTTTCCAGGGCCAGGAAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTCCTAGGGCCAGGTTTATTCCCTCACATGGGGGGTTCACAT ACACAGCACAGAGGCACGGGCACCATGGGAAAGGGCAGCACTCCTGCCTTTTGAGGGGAT CTTGGCCTCACGGGGTAAAAAGGGAAAGGATGGTTTCTCTTCTGCCCTCACTAGGGCCTA GGGAACCCAGGAGCAAATCCCACCACGCCTTCCATTTTTAAGCCAAGGAAAAGCCACCTT GGGGACGTTTAGTTCCAACCATTATAGTAAGGGGAAAAGGGATTGGCCTGGTCCCAACCA TTACAGGGGGAAGATATAAACAGTAAAGGAAGATACAGTTTGGATGAGGCCACAGGAAGG AGCAAATGACACCATCAAAACCATATGCAGGGAAAGGGCAGTTACTGGGCTTCTGGGCTG CTTANTCCCTGGCTTGGCAGGAAGGGTAGGGAAAATGGATGGGGCTCATTGTTTGGCATT GATGATGTCCACAAATTCGGGCTTGAGGGAAGCACCACCCACAAGGAAGCCATCCACATC AGGCTGGCTGGCCAGCTCCTTGCAGGTTGCCCCAGTCACAGAGCCTCCATAAATGATACG GGTGCTCTGAGCCACCGATNAGAGACGTTGGACTTCAGCCTCCTCGGAGCTTCTCGGTAC TTCTGGGCTGTTTNGGGGGTNTTTAAATTTTGCCAGTACCAATGGCCCACACAGCTCATA GCCAGACACTTGCTCANCCTTCAGTTATTGGAAGGACTTTGTTGCTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000365 |
| Insert Size | 1340 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000365.4, NP_000356.1 |
| RefSeq Size | 1254 bp |
| RefSeq ORF | 750 bp |
| Locus ID | 7167 |
| UniProt ID | P60174 |
| Domains | TIM |
| Protein Pathways | Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Inositol phosphate metabolism, Metabolic pathways |
| Gene Summary | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] Transcript Variant: This variant (1) encodes the predominant isoform (1). CCDS Note: This CCDS represents a TPI variant that uses an internal promoter compared to the CCDS53740.1 representation. Data in PMIDs 4022011, 2925688, 2243103 and 10575546 support the presence of the internal promoter used by this variant. The resulting isoform is 37 aa shorter at the N-terminus compared to the CCDS53740.1 isoform. N-terminal sequencing in PMIDs 9150946 and 9150948 supports the existence of the shorter isoform in vivo. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Four key steps control glycolytic flux in mammalian cells
,null,
Cell systems
,PubMed ID 29960885
[TPI1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202652 | TPI1 (Myc-DDK-tagged)-Human triosephosphate isomerase 1 (TPI1), transcript variant 1 |
CNY 2,400.00 |
|
| RC202652L1 | Lenti ORF clone of Human triosephosphate isomerase 1 (TPI1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202652L2 | Lenti ORF clone of Human triosephosphate isomerase 1 (TPI1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC202652L3 | Lenti ORF clone of Human triosephosphate isomerase 1 (TPI1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202652L4 | Lenti ORF clone of Human triosephosphate isomerase 1 (TPI1), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG202652 | TPI1 (tGFP-tagged) - Human triosephosphate isomerase 1 (TPI1), transcript variant 1 |
CNY 4,000.00 |
|
| SC320334 | TPI1 (untagged)-Human triosephosphate isomerase 1 (TPI1), transcript variant 1 |
CNY 2,400.00 |
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