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RRM1 (NM_001033) Human Untagged Clone
CAT＃: SC111007
RRM1 (untagged)-Human ribonucleotide reductase M1 (RRM1)
CNY 8,712.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | R1; RIR1; RR1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111007 sequence for NM_001033 edited (data generated by NextGen Sequencing)
ATGCATGTGATCAAGCGAGATGGCCGCCAAGAACGAGTCATGTTTGACAAAATTACATCT CGAATCCAGAAGCTTTGTTATGGACTCAATATGGATTTTGTTGATCCTGCTCAGATCACC ATGAAAGTAATCCAAGGCTTGTACAGTGGGGTCACCACAGTGGAACTAGATACTTTGGCT GCTGAAACAGCTGCAACCTTGACTACTAAGCACCCTGACTATGCTATCCTGGCAGCCAGG ATCGCTGTCTCTAACTTGCACAAAGAAACAAAGAAAGTGTTCAGTGATGTGATGGAAGAC CTCTATAACTACATAAATCCACATAATGGCAAACACTCTCCCATGGTGGCCAAGTCAACA TTGGATATTGTTCTGGCCAATAAAGATCGCCTGAATTCTGCTATTATCTATGACCGAGAT TTCTCTTACAATTACTTCGGCTTTAAGACGCTAGAGCGGTCTTATTTGTTGAAGATCAAT GGAAAAGTGGCTGAAAGACCACAACATATGTTGATGAGAGTATCTGTTGGGATCCACAAA GAAGACATTGATGCAGCAATTGAAACATATAATCTTCTTTCTGAGAGGTGGTTTACTCAT GCTTCGCCCACTCTCTTCAATGCTGGTACCAACCGCCCACAACTTTCTAGCTGTTTTCTT CTGAGTATGAAAGATGACAGCATTGAAGGCATTTATGACACTCTAAAGCAATGTGCATTG ATTTCTAAGTCTGCTGGAGGAATTGGTGTTGCTGTGAGTTGTATTCGGGCTACTGGCAGC TACATTGCTGGGACTAATGGCAATTCCAATGGCCTTGTACCGATGCTGAGAGTATATAAC AACACAGCTCGATATGTGGATCAAGGTGGGAACAAGCGTCCTGGGGCATTTGCTATTTAC CTGGAGCCTTGGCATTTAGACATCTTTGAATTCCTTGATTTAAAGAAGAACACAGGAAAG GAAGAGCAGCGTGCCAGAGATCTTTTCTTTGCTCTTTGGATTCCGGATCTCTTCATGAAA CGAGTGGAGACTAATCAGGACTGGTCTTTGATGTGTCCAAATGAGTGTCCTGGTCTGGAT GAGGTTTGGGGAGAGGAATTTGAGAAACTATATGCAAGTTATGAGAAACAAGGTCGTGTC CGCAAAGTTGTAAAAGCTCAGCAGCTTTGGTATGCCATCATTGAGTCTCAGACGGAAACA GGCACCCCGTATATGCTCTACAAAGATTCCTGTAATCGAAAGAGCAACCAGCAGAACCTG GGAACCATCAAATGCAGCAACCTGTGCACAGAAATAGTGGAGTACACCAGCAAAGATGAG GTTGCTGTTTGTAATTTGGCTTCCCTGGCCCTGAATATGTATGTCACATCAGAACACACA TACGACTTTAAGAAGTTGGCTGAAGTCACTAAAGTCGTTGTCCGAAACTTGAATAAAATT ATTGATATAAACTACTATCCTGTACCAGAGGCATGCCTATCAAATAAACGCCATCGCCCC ATTGGAATTGGGGTACAAGGTCTGGCAGATGCTTTTATCCTGATGAGATACCCTTTTGAG AGTGCAGAAGCCCAGTTACTGAATAAGCAGATCTTTGAAACTATTTATTATGGTGCTCTG GAAGCCAGCTGTGACCTTGCCAAGGAGCAGGGCCCATACGAAACCTATGAGGGCTCTCCA GTTAGCAAAGGAATTCTTCAGTATGATATGTGGAATGTTACTCCTACAGACCTATGGGAC TGGAAGGTTCTCAAGGAGAAGATTGCAAAGTATGGTATAAGAAACAGTTTACTTATTGCC CCGATGCCTACAGCTTCCACTGCTCAGATCCTGGGGAATAATGAGTCCATTGAACCTTAC ACCAGCAACATCTATACTCGCAGAGTCTTGTCAGGAGAATTTCAGATTGTAAATCCTCAC TTATTGAAAGATCTTACCGAGCGGGGCCTATGGCATGAAGAGATGAAAAACCAGATTATT GCATGCAATGGCTCTATTCAGAGCATACCAGAAATTCCTGATGACCTGAAGCAACTTTAT AAAACTGTGTGGGAAATCTCTCAGAAAACTGTTCTCAAGATGGCAGCTGAGAGAGGTGCT TTCATTGATCAAAGCCAATCTTTGAACATCCACATTGCTGAGCCTAACTATGGCAAACTC ACTAGTATGCACTTCTACGGCTGGAAGCAGGGTTTGAAGACTGGGATGTATTATTTAAGG ACAAGACCAGCAGCTAATCCAATCCAGTTCACTCTAAATAAGGAGAAGCTAAAAGATAAA GAAAAGGTATCAAAAGAGGAAGAAGAGAAGGAGAGGAACACAGCAGCCATGGTGTGCTCT TTGGAGAATAGAGATGAATGTCTGATGTGTGGATCCTGA Clone variation with respect to NM_001033.3 2232 g=>a >OriGene 5' read for NM_001033 unedited
GCACGAGGGCCTCTGCTCTGAAGAAGTGCTGTCTGGCTCCAACTCCAGTTCTTTCCCCTG AGCAGCGCCTGGAACCTAACCCTTCCCACTCTGTCACCTTCTCGATCCCGCCGGCGCTTT AGAGCCGCAGTCCAGTCTTGGATCCTTCAGAGCCTCAGCCACTAGCTGCGATGCATGTGA TCAAGCGAGATGGCCGCCAAGAACGAGTCATGTTTGACAAAATTACATCTCGAATCCAGA AGCTTTGTTATGGACTCAATATGGATTTTGTTGATCCTGCTCAGATCACCATGAAAGTAA TCCAAGGCTTGTACAGTGGGGTCACCACAGTGGAACTAGATACTTTGGCTGCTGAAACAG CTGCAACCTTGACTACTAAGCACCCTGACTATGCTATCCTGGCAGCCAGGATCGCTGTCT CTAACTTGCACAAAGAAACAAAGAAAGTGTTCAGTGATGTGATGGAAGACCTCTATAACT ACATAAATCCACATAATGGCAAACACTCTCCCATGGTGGCCAAGTCAACATTGGATATTG TTCTGGCCAATAAAGATCGCCTGAATTCTGCTATTATCTATGACCGAGATTTCTCTTACA ATTACTTC >OriGene 3' read for NM_001033 unedited
CCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAAAGCTTATTTTGGT GAAAATCCCAATATATGAGTTTAAAAAAAAATCATTAACATCATTAACAGTACTTTAAAT CAATTACTCCTTTTGCCTGCAACAGGGTCCAGCAAAGCCTTACCACCTCAAGCAAACCCA CTATACCTATCTATGCTCAAGAAGTAGTTTGGCTACTGAAGACATGCTGGTCTCTTCCAA GTCTTTCCTCAGGATCCACACATCAGACATTCATCTCTATTCTCCAAAGAGCACACCATG GCTGCTGGGTTCCTCTCCTTCTCTTCTTCCTCTTTGGATACCTTTTCTTTATCTTTTAGC TTCTCCTTATTTAGAGTGAACTGGATTGGATTAGCTGCTGGTCTTGTCCTTAAATAATAC ATCCCAGTCTTCAAACCCTGCTTCCAGCCGTAGAAGTGCATACTAGTGAGTTTGCCATAG TTAGGCTCAGCAATGTGGATGTTCAAAGATTGGCTTTGATCAATGAAAGCACCTCTCTCA GCTGCCATCTTGAGAACAGTTTTCTGAGAGATTTCCCACACAGTTCTATAAAGTTGCTTC AGGTCATCAGGAATTTCCTGGTATGCTCTGAATAGAGCCATTGCATGGCATAATCTGGTT TTTCATCTCTTCATGCCATAGGCCCCGCTCGGGAAGACCTTTAATTAGTGAGGATTTACA TTCTGAAATCCTCCTGACCAGACCCTGCAAGTATAGATGTGCTGTGGTAGGGTCCAAGGA CCCATTATTCCCACGACTGAACAGGGGACCCGATAGCTCCGGGCAATAATAAACGGTTCT TAACCATACTTGCAATTTTTCCTGGAAACCTCCAGTCCATAGGCTGTAGGGGACCTTCCC AATTAACCGGAAATTCCTTGCACCGGAAGCCCTAAGGTTTTATGCCTTGTCCTGGAAGGG ACCCGGCTTCAAGCCATAAAAAGTAAAAACGGTTTTAAACGGGCTGGCCCCAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001033 |
| Insert Size | 2930 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001033.2, NP_001024.1 |
| RefSeq Size | 3117 bp |
| RefSeq ORF | 2379 bp |
| Locus ID | 6240 |
| UniProt ID | P23921 |
| Domains | ribonuc_red_lg, ATP-cone |
| Protein Families | Druggable Genome |
| Protein Pathways | Glutathione metabolism, Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Bcl2 induces DNA replication stress by inhibiting ribonucleotide reductase
,Xie, M;Yen, Y;Owonikoko, TK;Ramalingam, SS;Khuri, FR;Curran, WJ;Doetsch, PW;Deng, X;,
Cancer Res.
,PubMed ID 24197132
[RRM1]
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