Myosin (MYL5) (NM_002477) Human Untagged Clone

CAT#: SC110955

MYL5 (untagged)-Human myosin, light chain 5, regulatory (MYL5)



  "NM_002477" in other vectors (6)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MYLC2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002477, the custom clone sequence may differ by one or more nucleotides


ATGGCCAGCAGGAAGACCAAGAAGAAGGAAGGGGGTGCCCTCCGGGCCCAGAGAGCCTCATCCAATGTCT
TCTCCAACTTTGAGCAGACTCAGATCCAGGAGTTCAAGGAGGCATTCACACTCATGGATCAGAACCGAGA
TGGCTTCATTGACAAGGAGGACCTGAAGGACACCTATGCCTCCCTGGGCAAGACCAACGTCAAGGACGAC
GAGCTGGACGCCATGCTCAAAGAGGCCTCGGGGCCCATCAACTTCACCATGTTTCTGAACCTGTTTGGGG
AGAAGCTGAGCGGTACCGACGCCGAGGAGACCATTCTTAACGCCTTCAAGATGCTGGACCCGGACGGGAA
AGGGAAAATCAACAAGGAGTACATCAAGCGTCTGCTGATGTCCCAGGCTGACAAGATGACGGCGGAAGAG
GTGGACCAGATGTTCCAGTTCGCCTCCATCGATGTGGCGGGCAACCTGGACTACAAGGCGCTCAGCTACG
TGATCACCCACGGGGAGGAGAAGGAGGAGTGA


>OriGene 5' read for NM_002477 unedited
TGTACTACGATTCACTATAGGGCGGCCCGCGATTCGGCACGAGGTGAGCGTGTGTATGTG
CGTGTGTGTGACCTTGCGGGTGTGGGCTGTGCTGTGTTGTGGGAAGTACGTGCCTCACGT
TGCTCTCCTGGTGAGAGTCCGGAGCAGGATGAGGGGGTTCCTGGCTTTCACAAAATCCCG
GTACCCAGAACAAGCCCACCCAGAGTCCACTTGCCCCTCAAGCCTTCAGAGGCCAAGCTG
GCTCCTGCTGGACGGCGATCCCTGACCACTGTGCTCTGTGGGCCTTCTGGAAGCCACTGT
CCCTCCCCCAGTCCCTGTGCTGAAGCCAGACACCTGCAGCCGTCCTGCCCCCAACCCCAG
CTACCCAGGCCTGAGGCTGGCATGCTCCTCAGCTGTCTGGCCCCCTCCAGCCCGAAGGGC
TGAGGTCCACCCTTCATCTGAGTTAAGTCTCTCAAAACTCTGGGTGCCCTGGAGGGTTTC
GTCAGCCAGCCCAGGACCCTCAGCCATGGTGCTCCCACCGCAGGCCAGCAGGAAGACCAA
GAAGAAGGAAGGGGGTGCCCTCCGGGCCCAGAGAGCCTCATCCAATGTCTTCTCCAACTT
TGAGCAGACTCAGATCCAGGAGTTCAAGGAGGCATTCACACTCATGGATCAGAACCGAGA
TGGCTTCATTGACAAGGAGGACCTGAAGGACACCTATGCCTCCCTGNGCAAGACCAACGT
CAAGGACGACGAGCTGGACGCCATGCTCNAAGAGCCTCGGGGCCCATCAACTTCACCATG
TTTCCTGACCTGTTTGGGGAGAAGCTGAGCGTTACCGACGCCCGAGAGACATTCTTNACG
CCTTCAGATGCTNGACCCGNCAGGGAAAGGAAATCACAAGAGTACTCACGCGCTGCTGAG
TCCCAGCTGACAGATGACGCGAC
>OriGene 3' read for NM_002477 unedited
GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGGG
TCCAAGCGTCCAGGTTTATTGACCCGGCTGGGTCTCACTCCTCCTTCTCCTCCCCGGGGG
TGATCACGTAGCTGAGCGCCTTGTAGTCCAGGTTGCCCGCCACATCGATGGAGGCGAACT
GGAACATTTGGTCCACCTTTTCCGCCGTCATCTTGTCAGCCTGGGACATCAGCAGACGCT
TGATGTACTCCTTGTTGATTTTCCCTTTCCCGTCCGGGTCCAGCATCTTGAAGGCGTTAA
AAATGGTCTCCTCGGCGTCGGTACCGCTCAGCTTCTCCCCAAACAGGTTCAAAAACATGG
TGAAGTTGATGGGCCCCGAGGCCTCTTTGAGCATGGCGTCCAGCTCGTCGTCCTTGACGT
TGGTCTTGCCCAGGGAGGCATAGGTGTCCTTCAGGTCCTCCTTGTCAATGAAGCCATCTC
GGTTCTGATCCATGAGTGTGAATGCCTCCTTGAACTCCTGGATCTGAGTCTGCTCAAAGT
TGGAGAAGACATATGGATGAGGCTCTCTGGGCCCGGAGGGCACCCCCTTCCTTCTTCTTG
GTCTTCCTGCTGGCCTGCGGTGGGAGCACCATGGCTGAGGGTCCTGNGCTGGCTGACGAA
ACCCTCCAGGGCACCCAGAGTTTTGAGAGACTTAACTCAGATGAAGGGTGGACCTCAGCC
CTTTCGGCTGGAGGGGGCCANACAGCTGAGGAGCATGCCAACCTCAGGCCTGGGTTACCT
GGGGTTGGGGGCAAGACGGCTGCCAGTTTTTGGCTTAACACAGGCACTTGGGGATGCACA
GTTGGTTCCAAAAGCCCACCAGACCCAGGGGTCAAGTATCGCCCTCCACCAAGAACCACC
TTGGCTTCTGAAGCTTGAGGGGAAGTGGATCTTGGTCGCGCTT
Restriction Sites NotI-NotI     
ACCN NM_002477
Insert Size 1060 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002477.1, NP_002468.1
RefSeq Size 661 bp
RefSeq ORF 522 bp
Locus ID 4636
UniProt ID Q02045
Domains EFh
Protein Pathways Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
Gene Summary This gene encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene product, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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