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EWSR1 (NM_013986) Human Untagged Clone
CAT＃: SC110886
EWSR1 (untagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1
CNY 4,352.00
Product images
CNY 1,999.00
CNY 3,490.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bK984G1.4; EWS; EWS-FLI1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_013986, the custom clone sequence may differ by one or more nucleotides
ATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCC AGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCC CACTGATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTAT GGACAGCCTCCCACTGTAGAAGGGACCAGTACAGGTTATACTACTCCAACTGCCCCCCAGGCATACAGCC AGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTC CTATGCAGCTCAGTCTGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACT GCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTT ACAACCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCAT GCAGCCAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGAT CAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTC AACAAAGCAGCTATGGGCAGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCC AAGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACCACCCCAGTAGC ATGGGTGTTTATGGGCAGGAGTCTGGAGGATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTG ATAACCGGGGCAGGGGAAGAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCGGGGAGGAGGACG CGGTGGAATGGGCGCTGGAGAGCGAGGTGGCTTCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGAT CTTGATCTAGGCCCACCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACAAGGATTAA ATGACAGTGTGACTCTAGATGATCTGGCAGACTTCTTTAAGCAGTGTGGGGTTGTTAAGATGAACAAGAG AACTGGGCAACCCATGATCCACATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTG TCCTATGAAGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATTTTCAAGGGAGCA AACTTAAAGTCTCCCTTGCTCGGAAGAAGCCTCCAATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGA GGGCAGAGGCATGCCACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGACCCATGGGT CGCATGGGAGGCCGTGGAGGAGATAGAGGAGGCTTCCCTCCAAGAGGACCCCGGGGTTCCCGAGGGAACC CCTCTGGAGGAGGAAACGTCCAGCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCA GAACTTCGCCTGGAGAACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAAGGCTTCCTCCCGCCACCC TTTCCGCCCCCGGGTGGTGATCGTGGCAGAGGTGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCA TGGATCGTGGTGGTCCCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGTGGTGG CCGGGGCATGGACCGAGGTGGCTTTGGTGGAGGAAGACGAGGTGGCCCTGGGGGGCCCCCTGGACCTTTG ATGGAACAGATGGGAGGAAGAAGAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTC AGGAGCGCAGAGATCGGCCCTACTAG >OriGene 5' read for NM_013986 unedited
NGGGTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGACGT TGAGAGAACGAGGAGGAAGGAGAGAAAAGGCGTCCACGGATTACAGTACCTATAGCCAAG CTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGA CCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCT ATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTATGGAC AGCCTCCCACTGGTTATACTACTCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGG GGTATGGCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCT ATGCAGCTCAGTCTGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAG CAGCCACTGCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTC AATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTT ATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACCTCCATCCTACCCTC CTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGA ACACCTATGGGCAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCT ATGGGCAGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCAA GTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACC >OriGene 3' read for NM_013986 unedited
CTATGAACCGCGGCCCGCTTCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTAACATTTGGAAATTTTATTTAAAAAAAAAAAAAAAACATCACAACCATGAACATTGT TACAGTTAAAAGGCCCTTTTGGTTCTCCACAATGATACTGAGCATGCTCACAAGGGGTTC CCATTGTTAAAGTCTTAAACAACCATTTTTAAAAGAAGGAAAAAAAAAAAACTCCGCACA CTACCATTTAACTTGTTTTAATGTTTCTTCACAAAGGGGGAAAAATACTAAAGTCCAGAC AAGGAATAATCATAATGTTGGGGCCAACATTATAAATATGGAATTATAAATTTAAAACAT TTTCTGGTTTAAAAAATAAACCTGGTACCCAATGCAGCTCTGCGGGGCCTCTGCATCTAG TAGGGCCGATCTCTGCGCTCCTGACGGGGCTCGCCTTTATCCATTTTTCCAGGTCCTCCA CGTCCTCCTTTTCTTCCTCCCATCTGTTCCATCAAAGGTCCAGGGGGCCCCCCAGGGCCA CCTTGTTTTCCTCCACCAAAGCCACCTGGGTCCATGCCCCGGCCACCACGGAAGCCACCT CTGTCTCCACCACGGCCACTTCTGAACATTCCACCGGGACCACCACGATCCATGAGGCCA CCTCTTCCTCCCCGCATGCCACCAGGGCCACCCTTTGCCACGATCACCACCCGGGGGCGG AAAAGGTGGCGGAAGGAACCCTTCAGGCTTGGGGGCCTTACACTGGTTGCACTCTGTTCT CCAGGCGAAGTTCTGGTTTCCACCACCCCGGATTGGGACCCTGCCAGTTCCCACCTGGGC GCTGGACCGTTTCTCCTCCACAAGGGTTTCCCTTCGGAAACCCCGGGTCCTTTTGGAGGA AAGCCTCTTTTTTTTCCTCCACGGCTCCCATGCGAACCATGTGGCCCCCAGGACCTTCTG GGCCTCCGAACCTCCGGGAGGGGGTGGCATCCTCTGCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013986 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013986.1, NP_053733.1 |
| RefSeq Size | 2694 bp |
| RefSeq ORF | 1752 bp |
| Locus ID | 2130 |
| UniProt ID | Q01844 |
| Domains | RRM, zf-RanBP |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Gene Summary | This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223794 | EWSR1 (Myc-DDK-tagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1 |
CNY 4,344.00 |
|
| RC223794L1 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1, Myc-DDK-tagged |
CNY 6,744.00 |
|
| RC223794L2 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| RC223794L3 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC223794L4 | Lenti ORF clone of Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1, mGFP tagged |
CNY 6,744.00 |
|
| RG223794 | EWSR1 (tGFP-tagged) - Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1 |
CNY 5,944.00 |
|
| SC327962 | EWSR1 (untagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1) transcript variant 1 |
CNY 10,550.00 |
