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E2F3 (NM_001949) Human Untagged Clone
CAT＃: SC110876
E2F3 (untagged)-Human E2F transcription factor 3 (E2F3)
CNY 5,488.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | E2F-3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110876 sequence for NM_001949 edited (data generated by NextGen Sequencing)
ATGAGAAAGGGAATCCAGCCCGCTCTGGAGCAGTACCTGGTGACCGCCGGGGGTGGGGAG GGGGCGGCTGTCGTCGCCGCCGCCGCTGCAGCCTCCATGGACAAAAGGGCACTGCTAGCC AGCCCCGGCTTCGCCGCCGCCGCCGCCGCTGCCGCCGCCCCGGGCGCGTACATCCAGATC CTCACCACGAACACTTCCACCACCTCCTGTTCCTCCTCCCTCCAAAGCGGCGCCGTAGCC GCCGGCCCCCTCCTCCCCAGTGCCCCCGGCGCGGAGCAGACCGCCGGCAGCCTCCTCTAC ACCACGCCGCACGGACCCTCCAGCAGAGCCGGGCTGCTGCAGCAGCCACCAGCGCTGGGA CGCGGCGGCAGCGGCGGCGGCGGCGGCCCTCCGGCAAAGCGAAGGCTGGAGCTAGGAGAA AGCGGTCATCAGTACCTCTCAGATGGTTTAAAAACCCCCAAGGGCAAAGGAAGAGCTGCA CTACGAAGTCCAGATAGTCCAAAAACTCCAAAATCTCCCTCAGAAAAAACGCGGTATGAT ACGTCTCTTGGTCTGCTCACCAAGAAGTTCATTCAGCTCCTGAGCCAGTCACCCGATGGG GTATTGGATTTGAACAAGGCAGCAGAAGTGCTAAAAGTGCAAAAGAGAAGGATTTATGAT ATCACCAACGTTCTGGAAGGCATCCACCTCATTAAGAAGAAGTCTAAAAACAACGTCCAA TGGATGGGCTGCAGTCTGTCTGAGGATGGGGGCATGCTGGCCCAGTGTCAAGGCCTGTCA AAAGAAGTGACCGAGCTCAGTCAGGAAGAGAAGAAATTAGATGAACTGATCCAAAGCTGC ACCCTGGACCTCAAACTGTTAACCGAGGATTCAGAGAATCAAAGGTTAGCTTATGTTACA TATCAAGATATTCGAAAAATTAGTGGCCTTAAAGACCAAACTGTTATAGTTGTGAAAGCC CCTCCAGAAACAAGACTTGAAGTGCCTGACTCAATAGAGAGCCTACAAATACATTTGGCA AGTACCCAAGGGCCCATTGAGGTTTACTTATGTCCAGAAGAGACTGAAACACACAGTCCA ATGAAAACAAACAACCAAGACCACAATGGGAATATCCCTAAACCCGCTTCCAAAGACTTG GCTTCAACCAACTCAGGACATAGCGATTGCTCAGTTTCTATGGGAAACCTTTCTCCTCTG GCCTCCCCAGCCAACCTCTTACAGCAGACTGAGGACCAAATTCCTTCCAACCTAGAAGGA CCGTTTGTGAACTTACTGCCTCCCCTGCTGCAAGAGGACTATCTCCTGAGCCTCGGGGAG GAGGAAGGCATCAGCGATCTCTTCGATGCTTACGATTTGGAAAAGCTCCCACTGGTGGAA GACTTCATGTGTAGTTGA Clone variation with respect to NM_001949.4 >OriGene 5' read for NM_001949 unedited
GGGTACTATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCGAGGGAAAAC AATACATTAATATACCATAACACTAAAAAGAGCAGGAGCGAGAGATGAGAAAGGGAATCC AGCCCGCTCTGGAGCAGTACCTGGTGACCGCCGGGGGTGGGGAGGGGGCGGCTGTCGTCG CCGCCGCCGCTGCATCCTCCATGGACAAAAGGGCACTGCTAGCCAGCCCCGGCTTCGCCG CCGCAGACCCTCATGACTCAACTTCTCCTCCTGCTACAGGCACCATATCACATCAAGCCC CATCCCTCTCTGCCTAGATCATACTTTCTCTCTTCCTCTCCCAACTACCTATCACTACGT TCCATCTCTAATTCAATTTGCGCACTCCACCAGATACCAACCCCTCCCACCGCTTCCCTA CCTCTCACCTCCTCAACCTTATCATTTTCTTTTACACAATCATCATCTTCTAACCCGCAC GATCCCCGTTCCCATACACTTTCACAACCCCTCTGACCGCCTCACCACTCACTCCTCGCT ACCATTGCTTCCCTTCTCCCCTTTTTACTACACTTCACTCCTCTTCTCTCTATCCATCTT AGCTCCCCTCTTTTTTACCTTATCCATACCTCTATTTCACTCCTCTCTTATGATTTTTTT CTTTTTTCTCCCATAACTATTTTCCTCTCCCCTTCCCTCACTCCCCTTCCTTTTACCTAC TCCGCACCCACTTTACTACTCCCCTCCTCAACACGCTCATTACTTCTCCTTCATTACGTG GTTTTCGTCCTCTTNTCCATCCTTTCCCTCTGTATTCCCAGTAGTTACTCCTCCTCTCTC CCCTACACAACTTTCCATCACCCANAAACACACACCCTCAGGCTCTTACTAGGCTCACCT CCTCAACTCTTATACCCTCACATTTTCATCCCCTTACACCTCTCTCTCCTCCTTCTGTTC TCATCCTCCCTCTCTTCTCCCCNTCTTGTCTATCCCACCTCCCTACGTGCTCCTTTCCGT ATCCTTTCCTCTCCTATATCTCCCACCGATCTC >OriGene 3' read for NM_001949 unedited
CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTAAGGATATTTTCAATTCAATTAAA CATATGAGTTGAGTGCCTACTATGTGCCTACCATTGGGTCAGGCACATAGCATTTCAATT TAACTGTTCGTTTCGAGGTTTGTTCTTTATCTGACTCCCTCATTCCCGCAGTCCTTATCA TCCAAGGAATGGACCGGAGATACTACGCCATATTCCCAGTGGGAGATGGCCGCTGCCACC TGCCCGAGGGAAAGCCATTCTTGGACATAACTGGTAGGATTTGCAGCTGACACAAAGCCT CCAACTTGGGACACCATTCCTGAAGAGTAAAGCGGGAGACACCCTGGCATTGTTTTGCTT CATTTTTTACGTCCCCGACTTCCCTTGGTCCAACTTATTCCTTCATAGAGGAAGGGGAAC GGGGTCTGGCCAGAACTATTCCAGCCCCCGACACAGGGCTGCTCCCTGTTCCTGCCAGCT TAAGGAAATGCCACTCACACAATGGGGATGTCTTTCTCCCTAGAGGTCTCTCCATCTGAC CCCATCCTGAGAAGTGGTCTCCCTGAGTGCGGTGTAAGAGAGCCCTTTCCCCTGTCACAG GGGCCCCGGGAAGCCCAAGGATGGAGGGACCCATGCCTGCTGCTCCTTGAGGCTGTTACA TCATCTGACCTGGTTCTCCTTTCCATGGGCCACCCATGTCAGCAAGCCCCATGACTGACT CGCTCCAAGCTCCCCCTNCTGGGAATGGNAGCACGATGGGACAAAGCTCCTTGCAGGAGC TGANTAACCCTANATCACACAGGTCTTAGATGCTCTGATCAGTAAAGGNTCTCTCCCTCT GCCTAATAATATGAATTNGCAAAACCCGCCTCAAAGTAAACATTTAAATCGGAATGGGGG ATGGCTGCCCCAGGAAGATGCTAATGGCACGCTAAAAAATTTAAAGCCTAGAACATCCCA AAAGAACTTAGTAGGCGTTTGCTACAATCANCTGGACTNCCAAACCATGGGGACGGGGAA TGGNATGGTCATGAAACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001949 |
| Insert Size | 3150 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001949.2, NP_001940.1 |
| RefSeq Size | 4744 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 1871 |
| UniProt ID | O00716 |
| Domains | E2F_TDP |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer |
| Gene Summary | This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] Transcript Variant: This variant (1, also known as a) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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MiR-34a Inhibits Viability and Invasion of Human Papillomavirus-Positive Cervical Cancer Cells by Targeting E2F3 and Regulating Survivin
,Geng, D;Song, X;Ning, F;Song, Q;Yin, H;,
Int. J. Gynecol. Cancer
,PubMed ID 25675046
[E2F3]
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Epstein-Barr Virus Downregulates MicroRNA 203 through the Oncoprotein Latent Membrane Protein 1: a Contribution to Increased Tumor Incidence in Epithelial Cells
,Haibo Yu, Jianhong Lu, Lielian Zuo, Qijia Yan, Zhengyuan Yu, Xiayu Li, Jin Huang, Lian Zhao, Hailin Tang, Zhaohui Luo, Qianjin Liao, Zhaoyang Zeng, Junyi Zhang, and Guiyuan Li,
J. Virol., Mar 2012; 86: 3088 - 3099.
[E2F3]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222471 | E2F3 (Myc-DDK-tagged)-Human E2F transcription factor 3 (E2F3) |
CNY 5,488.00 |
|
| RC222471L1 | Lenti ORF clone of Human E2F transcription factor 3 (E2F3), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC222471L2 | Lenti ORF clone of Human E2F transcription factor 3 (E2F3), mGFP tagged |
CNY 5,890.00 |
|
| RC222471L3 | Lenti ORF clone of Human E2F transcription factor 3 (E2F3), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC222471L4 | Lenti ORF clone of Human E2F transcription factor 3 (E2F3), mGFP tagged |
CNY 7,888.00 |
|
| RG222471 | E2F3 (tGFP-tagged) - Human E2F transcription factor 3 (E2F3) |
CNY 7,088.00 |
