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CLCN3 (NM_001829) Human Untagged Clone
CAT＃: SC110856
CLCN3 (untagged)-Human chloride channel 3 (CLCN3), transcript variant b
CNY 6,000.00
货期*
4周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ClC-3; CLC3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC110856 representing NM_001829.
Blue=Insert sequence Red=Cloning site Green=Tag(s) CTTTCCCAGTGTTCTAGTTCGCCCGTGACCCGGAATAATGAGCAAGGAGGGTGTGGTGGGTTGAAAGCC ATCCTACTTTACTCCCGAGTTAGAGCATGGATTCAGTTTTAGTCTTAAGGGGGAAGTGAGATTGGAGAT TTTTATTTTTAATTTTGGGCAGAAGCAGGTTGACTCTAGGGATCTCCAGAGCGAGAGGATTTAACTTCA TGTTGCTCCCGTGTTTGAAGGAGGACAATAAAAGTCCCACCGGGCAAAATTTTCGTAACCTCTGCGGTA GAAAACGTCAGGTATCTTTTAAATCGCGATAGTTTTCGCTGTGTCAGGCTTTCTTCGGTGGAGCTCCGA GGGTAGCTAGGTTCTAGGTTTGAAACAGATGCAGAATCCAAAGGCAGCGCAAAAAACAGCCACCGATTT TGCTATGTCTCTGAGCTGCGAGATAATCAGACAGCTAAATGGAGTCTGAGCAGCTGTTCCATAGAGGCT ACTATAGAAACAGCTACAACAGTATAACAAGTGCAAGTAGTGATGAGGAACTTTTAGATGGAGCAGGTG TTATTATGGACTTTCAAACATCTGAAGATGACAATTTATTAGATGGTGACACTGCAGTTGGAACTCATT ATACAATGACAAATGGAGGCAGCATTAACAGTTCTACACATTTACTGGATCTTTTGGATGAACCAATTC CAGGTGTTGGTACATATGATGATTTCCATACTATTGATTGGGTGCGAGAAAAATGTAAAGACAGAGAAA GGCATAGACGGATCAACAGCAAAAAGAAAGAATCAGCATGGGAAATGACAAAAAGTTTGTATGATGCGT GGTCAGGATGGCTAGTAGTAACACTAACAGGATTGGCATCAGGGGCACTGGCCGGATTAATAGACATTG CTGCCGATTGGATGACTGACCTAAAGGAGGGCATTTGCCTTAGTGCGTTGTGGTACAACCACGAACAGT GCTGTTGGGGATCTAATGAAACAACATTTGAAGAGAGGGATAAATGTCCACAGTGGAAAACATGGGCAG AATTAATCATAGGTCAAGCAGAGGGTCCTGGTTCTTATATCATGAACTACATAATGTACATCTTCTGGG CCTTGAGTTTTGCCTTTCTTGCAGTTTCCCTGGTAAAGGTATTTGCTCCATATGCCTGTGGCTCTGGAA TTCCAGAGATTAAAACTATTTTAAGTGGATTCATCATCAGAGGTTACTTGGGAAAATGGACTTTAATGA TTAAAACCATCACATTAGTCCTGGCTGTGGCATCAGGTTTGAGTTTAGGAAAAGAAGGTCCCCTGGTAC ATGTTGCCTGTTGCTGCGGAAATATCTTTTCCTACCTCTTTCCAAAGTATAGCACAAACGAAGCTAAAA AAAGGGAGGTGCTATCAGCTGCCTCAGCTGCAGGGGTTTCTGTAGCTTTTGGTGCACCAATTGGAGGAG TTCTTTTTAGCCTGGAAGAGGTTAGCTATTATTTTCCTCTCAAAACTTTATGGAGATCATTTTTTGCTG CTTTAGTGGCTGCATTTGTTTTGAGGTCCATCAATCCATTTGGTAACAGCCGTCTGGTCCTTTTTTATG TGGAGTATCATACACCATGGTACCTTTTTGAACTGTTTCCTTTTATTCTTCTAGGGGTATTTGGAGGGC TTTGGGGAGCCTTTTTCATTAGGGCAAATATTGCCTGGTGTCGTCGACGCAAGTCCACGAAATTTGGAA AGTATCCCGTTCTGGAAGTCATTATTGTTGCAGCCATTACTGCTGTGATAGCCTTCCCTAATCCATACA CTAGGCTAAACACCAGTGAACTGATCAAAGAGCTTTTTACAGACTGTGGTCCCCTGGAATCCTCTTCTC TTTGTGACTACAGAAATGACATGAATGCCAGTAAAATTGTCGATGACATTCCTGATCGTCCAGCAGGCA TTGGAGTATATTCAGCTATATGGCAGTTATGCCTGGCACTCATATTTAAAATCATAATGACAGTATTCA CTTTTGGCATCAAGGTTCCATCAGGCTTGTTCATCCCCAGCATGGCCATTGGAGCGATCGCAGGAAGGA TTGTGGGGATTGCGGTGGAGCAGCTTGCCTACTATCACCACGACTGGTTTATCTTTAAGGAGTGGTGTG AGGTCGGGGCTGATTGCATTACACCTGGCCTTTATGCCATGGTTGGTGCTGCTGCATGCTTAGGTGGTG TGACAAGAATGACTGTCTCCCTGGTGGTTATTGTTTTTGAGCTTACTGGAGGCTTGGAATATATTGTTC CCCTTATGGCTGCAGTCATGACCAGTAAATGGGTTGGAGATGCCTTTGGCAGGGAAGGCATTTATGAAG CACACATCCGATTAAATGGATACCCTTTCTTGGATGCAAAAGAAGAATTCACTCATACCACCCTGGCTG CTGACGTTATGAGACCTCGAAGGAATGATCCTCCCTTAGCTGTCCTGACACAGGACAATATGACAGTGG ATGATATAGAAAACATGATTAATGAAACCAGCTACAATGGATTTCCTGTCATAATGTCAAAAGAATCTC AGAGATTAGTGGGATTTGCCCTCAGAAGAGACCTGACAATTGCAATAGAAAGTGCCAGGAAAAAACAAG AAGGTATCGTTGGCAGTTCTCGGGTGTGTTTTGCACAGCACACCCCATCTCTTCCAGCAGAAAGTCCTC GGCCATTGAAGCTTCGAAGCATTCTTGACATGAGCCCTTTTACAGTGACAGACCACACCCCAATGGAGA TCGTGGTGGATATTTTCCGAAAGCTGGGACTGAGGCAGTGCCTTGTAACTCACAATGGGCGCCTCCTTG GCATTATAACAAAAAAAGATATCCTCCGGCATATGGCCCAGACGGCAAACCAAGACCCCGCTTCAATAA TGTTCAACTGA >OriGene 5' read for NM_001829 unedited
GCCCATTTAGTTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCTTTCCCAG TGTTCTAGTTCGCCCGTGACCCGGGATAATGAGCAAGGAGGGTGTGGTGGGTTGAAAGCC ATCCTACTTTACTCCCGAGTTAGAGCATGGATTCAGTTTTAGTCTTAAGGGGGAAGTGAG ATTGGAGATTTTTATTTTTAATTTTGGGCAGAAGCAGGTTGACTCTAGGGATCTCCAGAG CGAGAGGATTTAACTTCATGTTGCTCCCGTGTTTGAAGGAGGACAATAAAAGTCCCACCG GGCAAAATTTTCGTAACCTCTGCGGTAGAAAACGTCAGGTATCTTTTAAATCGCGATAGT TTTCGCTGTGTCAGGCTTTCTTCGGTGGAGCTCCGAGGGTAGCTAGGTTCTAGGTTTGAA ACAGATGCAGAATCCAAAGGCAGCGCAAAAAACAGCCACCGATTTTGCTATGTCTCTGAG CTGCGAGATAATCAGACAGCTAAATGGAGTCTGAGCAGCTGTTCCATAGAGGCTACTATA GAAACAGCTACAACAGTATAACAAGTGCAAGTAGTGATGAGGAACTTTTAGATGGAGCAG GTGTTATTATGGACTTTCAAACATCTGAAGATGACCATTTATTAGATGGTGACACTGCAG TTGGAACTCATTATACCATGACAAATGGAGGCAGCATTAACAGTTCTACACATTTACTGG ATCTTTTGGATGACCCATTCCAGTGTTGGTACATATGATGATTTCCATACTATTGGATGG GTGCCAGAAAATGCTAAGACAGAGAAGGCATATACGGGTCAACAGCAAAAAGAAGAATCA GCCTGGGAAATGACACAAGTTNGTTTGATGCGCTGTCCAGAGGCCTCTANTAACACTACC AGATGGCCTCAGGGCCCTGGCCCGATAATGGACACTGCG >OriGene 3' read for NM_001829 unedited
GGGTTAGCTTGNCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGTATTT TATAAATTTTAATCCATAAAAATATAAATAAGAATGGTAAATTTTTAAGAAAAAAAATAT ACATTTAGTGCAAGTTATGAATTACATTTCTTCTTCAGTGTCAGCCATGAGACATCTTGA GAAAAGAAAAGAATGACTGATCTAATTTCAATTTTAAAAATTCCAGTGTTTTAACATGCA ATTAATACCAGATACAGCTAATCAGAATACTTCCTTCTCTTTACTGTCAAAGCAGCAGCA TTTTATTTAATTATGCTCAACAGCACTTGTATATCATAGTAGTAGTCTTAGAAATAGAAA ATTTGCATAAGACCCGGTCACATTATCCATAAAGTCTGAGAGGAAAACAAAAAAGAAAAC TTTATCCTTTAAAACCAAGCTTCATTGTTTTCGGTTTTGATCCACACGGCACACAATGAG AGATTTCGTTCTCGCTTCCTTCGAGACTTTACCTGTTTGATGAGCCTTTGTTGTGGTGCA TGTGTACTGTTCCAAGCATTTAGTGATGGTACAAACTACATGGTTAATGGCACAAGAAAC ACTCCCTTCTTGTGATGAAACCTCAGTAAAATCACAGTACATCTCAATATGAGAAAGGAC TGCTTCAAAAGGATACACCGATTTAAAGAGGCTTTGTTACAGCTCACTCNAGAGAGAGAG AGAGAGAGAGAGAGAGAAATAAAGGCTCTTCTGGAATGTAGAAAGGGTATGGCTTAACTA TACAATTCATGAATTAAACAGAACAGTTTTGATTAAAAAAAAAGGCCAGCACCTTGCAAG TTTATAGATGGCTCAATTCCAAAGTATCCCTTCTAGAATAGGGACCTGATATGTGTCTTT GCATGTTGAACTCACAGCCCAGTGCTCGAGGACTCTGCTGTCATTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001829 |
| Insert Size | 2909 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001829.2 |
| RefSeq Size | 3982 bp |
| RefSeq ORF | 2909 bp |
| Locus ID | 1182 |
| UniProt ID | P51790 |
| Domains | CBS, voltage_CLC |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| MW | 110.9 kDa |
| Gene Summary | This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (b) represents the predominant transcript and encodes isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214128 | CLCN3 (Myc-DDK-tagged)-Human chloride channel 3 (CLCN3), transcript variant b |
CNY 8,650.00 |
|
| RC214128L3 | Lenti-ORF clone of CLCN3 (Myc-DDK-tagged)-Human chloride channel 3 (CLCN3), transcript variant b |
CNY 10,550.00 |
|
| RC214128L4 | Lenti-ORF clone of CLCN3 (mGFP-tagged)-Human chloride channel 3 (CLCN3), transcript variant b |
CNY 10,550.00 |
|
| RG214128 | CLCN3 (tGFP-tagged) - Human chloride channel 3 (CLCN3), transcript variant b |
CNY 9,500.00 |
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