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UBE3B (NM_183415) Human Untagged Clone
CAT＃: SC110718
UBE3B (untagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 3
CNY 7,448.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BPIDS; KOS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_183415, the custom clone sequence may differ by one or more nucleotides
ATGTTCACCCTGTCTCAGACCTCGAGAGCATGGTTCATCGATAGAGCCCGTCAGGCACGAGAAGAAAGGC TTGTGCAGAAGGAACGGGAGCGGGCAGCTGTTGTGATCCAGGCCCATGTCCGGAGTTTTCTCTGTCGGAG TCGACTGCAGAGAGATATCAGGAGAGAGATTGATGACTTTTTTAAAGCAGATGACCCTGAGTCCACTAAA AGAAGTGCACTTTGTATTTTCAAGATTGCCAGGAAACTGCTGTTCCTATTCAGAATCAAAGAGGATAATG AGAGATTTGAGAAGTTGTGTCGCAGCATCCTGAGCAGCATGGATGCTGAGAATGAGCCTAAGGTGTGGTA TGTGTCCCTGGCTTGTTCTAAGGACCTCACCCTCCTTTGGATTCAACAGATCAAGAACATTTTGTGGTAC TGCTGTGATTTTCTCAAGCAGCTCAAGCCTGAAATCCTGCAGGACTCCCGACTCATCACCCTGTACCTCA CGATGCTTGTCACCTTCACAGACACTTCAACGTGGAAAATTCTTCGGGGAAAAGGTGAAAGTCTTCGACC AGCGATGAACCACATTTGTGCAAATATAATGGGACATCTCAACCAGCATGGATTTTATTCTGTGCTGCAG ATATTGTTAACCCGTGGCCTGGCAAGACCCCGTCCTTGTCTATCCAAAGGCACTTTAACAGCAGCTTTTT CTCTAGCGTTACGCCCTGTGATTGCTGCACAGTTCTCAGACAATCTGATTCGGCCGTTCCTCATCCACAT CATGTCTGTGCCTGCTCTGGTGACTCATCTCAGCACAGTGACCCCTGAGCGCCTCACTGTTTTAGAATCC CATGACATGCTTCGTAAATTCATCATATTTTTAAGAGACCAAGATCGATGCCGTGATGTATGTGAAAGTT TAGAAGGATGCCATACGCTTTGTCTAATGGGCAACCTCCTACACTTGGGCTCCCTCAGCCCCAGAGTGTT AGAGGAGGAGACAGATGGGTTCGTGAGTTTGCTCACCCAGACGCTGTGCTACTGTCGGAAGTATGTGTCT CAGAAGAAGTCCAACCTGACCCACTGGCATCCTGTCCTTGGCTGGTTCTCCCAATCTGTGGATTATGGCC TTAACGAGTCAATGCACTTGATCACCAAACAGCTGCAGTTCTTGTGGGGGGTGCCTCTGATCCGGATCTT CTTCTGTGACATCCTGAGCAAGAAGCTACTGGAGAGCCAGGAGCCAGCCCACGCACAGCCAGCATCCCCT CAGAATGTGCTCCCAGTGAAGAGTCTCCTAAAGCGTGCTTTTCAAAAGTCGGCATCAGTCCGGAATATTC TCAGGCCTGTCGGGGGTAAACGGGTCGACTCTGCAGAAGTCCAGAAGGTTTGCAACATCTGTGTCCTCTA CCAGACCTCGCTGACAACTCTCACACAGATTCGGCTGCAGATACTCACAGGTCTCACTTACCTTGATGAC CTGCTTCCCAAACTGTGGGCATTTATCTGTGAGCTCGGGCCCCACGGAGGGTTAAAGCTCTTCTTGGAAT GCCTGAACAATGACACTGAAGAGTCCAAGCAACTCTTGGCCATGCTGATGCTGTTCTGTGACTGTTCGCG GCACCTCATCACAATCCTTGATGACATTGAAGTTTATGAAGAACAGATTTCATTCAAACTGGAAGAGCTG GTCACTATCTCCTCTTTCCTGAATTCTTTTGTGTTTAAGATGATCTGGGATGGAATTGTAGAGAACGCCA AGGGTGAGACCTTGGAGCTGTTCCAGTCTGTCCACGGGTGGCTTATGGTGCTGTACGAGCGGGACTGCCG GCGGCGCTTCACCCCCGAGGACCACTGGCTGCGAAAGGATCTCAAACCTAGCGTGCTCTTCCAAGAACTC GACAGGGACAGAAAACGGGCACAGTTGATCCTGCAGTACATCCCACATGTCATCCCTCACAAAAACAGAG TTCTACTGTTTCGAACCATGGTTACCAAGGAGAAGGAGAAACTGGGGCTGGTGGAAACCAGCTCTGCCTC CCCGCATGTCACTCACATCACCATCCGCCGGTCCAGGATGCTGGAGGACGGCTACGAGCAGCTTAGGCAG CTCTCCCAGCACGCCATGAAGGGGGTCATCCGTGTGAAGTTTGTCAATGACCTCGGGGTGGACGAAGCAG GGATTGATCAAGACGGTGTTTTTAAGGAGTTCTTGGAAGAGATCATCAAGAGAGTTTTTGACCCAGCACT CAATCTGTTCAAGACAACCAGTGGGGATGAGAGGCTGTACCCCTCACCCACATCCTACATCCATGAGAAT TACCTGCAGCTCTTCGAGTTTGTGGGGAAGATGCTGGGGAAGGCTGTGTATGAGGGAATTGTGGTGGACG TGCCATTTGCATCCTTCTTCCTGAGCCAACTGCTTGGGCACCACCACAGCGTCTTCTATAGCTCGGTGGA TGAACTGCCTTCTCTGGACTCCGAGTTCTATAAAAACCTCACCTCCATCAAGCGCTATGATGGGGACATC ACTGACCTGGGCCTGACGCTGTCTTACGACGAGGACGTCATGGGTCAGCTTGTTTGCCATGAACTGATTC CTGGAGGGAAGACCATTCCTGTTACAAATGAAAATAAAATTAGCTACATCCATCTGATGGCACATTTTCG AATGCACACTCAAATAAAAAACCAAACAGCTGCCCTCATTAGCGGATTCCGTTCCATTATCAAACCCGAG TGGATCCGAATGTTCTCAACTCCTGAACTGCAGCGTCTCATCTCTGGCGACAATGCTGAGATTGATCTGG AAGATTTAAAGAAGCACACAGTCTACTACGGTGGTTTCCATGGAAGTCACAGAGTCATCATCTGGCTCTG GGATATTCTGGCCTCCGACTTCACACCGGATGAGAGAGCTATGTTTCTGAAGTTCGTGACCAGCTGCTCC AGACCCCCGCTCCTGGGATTCGCCTACCTCAAGCCTCCATTCTCCATCCGCTGCGTGGAGGTGTCGGACG ATCAGGACACCGGGGACACTCTGGGCAGCGTCCTCCGGGGCTTCTTCACCATCCGCAAGCGGGAGCCAGG CGGCCGCCTGCCCACCTCCTCCACCTGCTTCAACCTGCTCAAGCTGCCCAACTACAGCAAGAAGAGCGTC CTCCGCGAGAAGCTGCGCTACGCCATCAGCATGAACACGGGCTTTGAACTCTCCTAG >OriGene 5' read for NM_183415 unedited
TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGTAGTGCGTCGGCT GCTGCCCCGGGTCTGGCAGAACTCGGGTGTTTTGGGCTGAGACAGTGGCAGCTGCGGCCC CGACCCCAAGTGCGGGGACCTCCGGCGAATAAAGAAAAAATAACAAGCTTTTCTGAAGTG AGAAGCTGTTCTCAGCCACGAGTCCTGTGCAAGATCACTAATGATTACCTGGCATTTCTG CGACACAGGCAGGTCCTCAGGGTTTGTGCAAGTTTGCAAACATGTTCACCCTGTCTCAGA CCTCGAGAGCATGGTTCATCGATAGAGCCCGTCAGGCACGAGAAGAAAGGCTTGTGCAGA AGGAACGGGAGCGGGCAGCTGTTGTGATCCAGGCCCATGTCCGGAGTTTTCTCTGTCGGA GTCGACTGCAGAGAGATATCAGGAGAGAGATTGATGACTTTTTTAAAGCAGATGACCCTG AGTCCACTAAAAGAAGTGCACTTTGTATTTTCAAGATTGCCAGGAAACTGCTGTTCCTAT TCAGAATCAAAGAGGATAATGAGAGATTNGAGAAGTTGTGTCGCAGCATCCTGAGCAGCA TGGATGCTGAGAATGAGCCTAAGGGTGTGGTNATGTGTCCCTGGGCTTGTTCTAAGGACC TCACCCTCCTTTGGATTCAACAGATAAGACATTTTTTGGGGGTACTGCTGGGGATTTTCT CAAGCAGCTCAAGCCTGAAATCCTGCAGGACTCCCGACTCATCACCCTGTACCTACGGAT GCTTGTCACCTTCACAGACCTTCACGGTGGGAAATTCTTCGGGGAAAGGGGGGGAAAGTT CTCGACCAGCGATGAACCACATTTGTGCAATATTAATGGGACATCTCACAGCTTGGATTT TTTTATTCTGGCTGGAAAAATTGTAC >OriGene 3' read for NM_183415 unedited
GTACTATGGACCGCGCGCCGCTTTCTANGATCGANATTTTTTTTTTTTTTTTTTAATATG CTGAATTACAAATTTAATAAAGCCTTTGATCACATCTCAATCCATAAGTTAGCTACAAAA ACAAAAAATCCTACAACTTTTTAGAGCCATCAAGAGCCATCAAGGTGTCAGTGAACACCA GTTACAAAAGGAGATTCAGTGTTTCAGATAGTGCAATATTTCCAGCTGCTGAAAAGAATT TAAATGAAATTACAAACTACCCCTCCTTGTCAAAAATCCACATGAAGTTGATATTGGTGC TTATAAATCACTCTCTCCCAGTCCCTCACTGGTTCCAACCTTCAGGTGATAAAAATTAGG ATGGGATCCATCTTCCCTGTGCTGACAGTCTGGGGTCCCCGCATGTATGCACGAACCCGC CCAGCGTGCGCACACACGTTCAGAAGAAATCTTCAAAGGAACCGAGCGTTTGGAGAAAGT GGCAAGTCCACAGAATCAGAGGTTACGAACACACCTTCAATAATATTAATACATTCCTGT CTTTAAATTCCTTGCCATGTTTCCATCAAAGTAGAGCACACACTCTTTTCCAGAAGCCTG GGGGCTCGACCTGGGTGGGACACCAGGATGCAGCTGAAACTCTGAAGGACTGTGGAGACG GGTGGCCCCTGCTTTGCTGCCACTGTGACAGCTCTTGTTGGAAAATGTTTTGCGTCTTTG AGGAGAGCAGAGGAGGGACTGGGCCTTCCTGGCTTCGAATCCGGCATCAACCTTGGTGGT GCAGGTGCAGGAGCATGAGGTGGAGAGAGGNGGNTTNCCTNNCAAACCNACCACAAACGC GGCTCCGCCTCACCCCCTGGAGCTAAGTTCTTACCACCAAGCCCAGCAGAGGTTATCAGA CCCATTGGTTGGAACACATGCACCTTACAAAACCAACACCCACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_183415 |
| Insert Size | 6050 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_183415.1, NP_904324.1 |
| RefSeq Size | 5404 bp |
| RefSeq ORF | 3207 bp |
| Locus ID | 89910 |
| UniProt ID | Q7Z3V4 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR, as compared to variant 1. Variants 1 and 3 encode the same protein (isoform 1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223833 | UBE3B (Myc-DDK-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 3 |
CNY 7,440.00 |
|
| RC223833L3 | Lenti-ORF clone of UBE3B (Myc-DDK-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 3 |
CNY 10,260.00 |
|
| RC223833L4 | Lenti-ORF clone of UBE3B (mGFP-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 3 |
CNY 10,260.00 |
|
| RG223833 | UBE3B (tGFP-tagged) - Human ubiquitin protein ligase E3B (UBE3B), transcript variant 3 |
CNY 9,040.00 |
