ABHD11 (NM_031295) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	abhydrolase domain containing 11; PP1226; PP1226, WBSCR21; WBSCR21; Williams Beuren syndrome chromosome region 21
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_031295, the custom clone sequence may differ by one or more nucleotides ATGCGAGCCGGCCAACAGCTTGCAAGCATGCTCCGCTGGACCCGAGCCTGGAGGCTCCCGCGTGAGGGAC TCGGCCCCCACGGCCCTAGCTTCGCGAGGGTGCCTGTCGCACCCAGCAGCAGCAGCGGCGGCCGAGGGGG CGCCGAGCCGAGGCCGCTTCCGCTTTCCTACAGGCTTCTGGACGGGGAGGCAGCCCTCCCGGCCGTCGTC TTTTTGCACGGGCTCTTCGGCAGCAAAACTAACTTCAACTCCATCGCCAAGATCTTGGCCCAGCAGACAG GCCGTGCTGACGGTGGATGCTCGTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_031295 unedited GTCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGTGAGATGCGA GCCGGCCAACAGCTTGCAAGCATGCTCCGCTGGACCCGAGCCTGGAGGCTCCCGCGTGAG GGACTCGGCCCCCACGGCCCTAGCTTCGCGAGGGTGCCTGTCGCACCCAGCAGCAGCAGC GGCGGCCGAGGGGGCGCCGAGCCGAGGCCGCTTCCGCTTTCCTACAGGCTTCTGGACGGG GAGGCAGCCCTCCCGGCCGTCGTCTTTTTGCACGGGCTCTTCGGCAGCAAAACTAACTTC AACTCCATCGCCAAGATCTTGGCCCAGCAGACAGGCCGTAGGGTGCTGACGGTGGATGCT CGTAACCACGGTGACAGCCCCCACAGCCCAGACATGAGCTACGAGATCATGAGCCAGGAC CTGCAGGACCTTCTGCCCCAGCTGGGCCTGGTGCCCTGCGTCGTCGTTGGCCACAGCATG GGAGGAAAGACAGCCATGCTGCTGGCACTACAGAGGCCAGAGCTGGTGGAACGTCTCATT GCTGTAGATATCAGCCCAGTGGAAAGCACAGGTGTCTCCCACTTTGCAACCTATGTGGCA GCCATGAGGGCCATCAACATCGCAGATGAGCTGCCCCGCTCCCGTGCCCGAAAACTGGCG GATGAACAGCTCAGTTCTGTCATCCAGGACATGGCCGTGCGGCAGCACCTGCTCACTAAC CTGGTAGAGGTAGACGGGCGCCTTCTGTGGAGGGTGAACTTGGATGCCCTGACCCAGCAC CTAGACAAGATCTTGCTTTTCCCACAAAGCAGGAGTCCTACCCGGGGCCACACTTTTTCT CCTTGTTGGAACCTCCAGTTTGGGCCTTCCACCCCAACCTGGAATATGCGGCTCTTCCCC GG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_031295 unedited GCTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAGCTAAT TTTTGTATTTTTAGTAAAGAAGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTG GCCTCAAGTCATCCTCCCGCCTTATCCTCCCAAAGTGCTGGAATTACAGGCATGAGCCAC CACGCCCGGCCATCTTCTTGCCAGCAACTCTTATACCAGGAAGCCTCGGATGGCAGCTAT GAAGCCCTGTGGGCGGTCAGCGTGGATCCAGTGGCCAGCGTTCGGCACCGTCTGCATCTG GGCCCGAGGGAAGAGCCGCATAATCTCAGGGGGGTGGCTGGGATGCACGAACTGGGAGTT TCCACCAAGGAGAAAGAGTGTTGGCCCGAGGTAGGACTCCTGCCTCTGTGGGAAAGCCAA GATCTTGCCTAGGCGCTGGGCCAGGGCATCCAAGTTCACCCTCCACACGAAGCGCCCGTC TACCTCTACCAGGTTAGGGAGCAGGTGCTGCCGCACGCCCATGTCCTGAATGACAGACCT GACCTGTCCATCCGCAGTTTTCGGGCCCGGAACCGGGGCACCTATCTGCGATGTGGAGGC CCCCATGGCTGCCCCAAGGCTCGAAAGTGGGAAAACCTGGGCCTTCCACTGGGCTGATAT CAACACATGGAAACCTTCCACCAGCTCGGCCCTTGGAGGCCAACAACAAGGCTGCTTTTC CTCCAAGCGTGCGCCCACGACAACCAAGGCCCCAGGCCAATTGGGGCAAAGGGCCCGCAG GCCCTGGCCTATGACCCCAAATCAAGCCCGGCCGGGGGGCCGACACCGGGGTACAAACTT CCCCGTAGAACCCTCAGCGCGGTCGTGGGGCCAAACTTGACAAGGAGTAAAATATCTCGC CGCACAACCCCACCAAAAAACCGCTCGGCGCCCACCCTCCCCCAACCGGTGGAN
Restriction Sites	NotI-NotI
ACCN	NM_031295
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031295.3, NP_112585.2
RefSeq Size	1667 bp
RefSeq ORF	1632 bp
Locus ID	83451
Gene Summary	This gene encodes a protein containing an alpha/beta hydrolase fold domain. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (4) contains an alternate segment in the 3' UTR, and lacks a short segment in the coding region, which causes a frameshift, as compared to variant 1. It encodes a much shorter isoform (4), also known as form B, than isoform 1.

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