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ARFGAP1 (NM_018209) Human Untagged Clone
CAT＃: SC110523
ARFGAP1 (untagged)-Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1
CNY 3,656.00
CNY 6,560.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARF1GAP; HRIHFB2281 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110523 sequence for NM_018209 edited (data generated by NextGen Sequencing)
ATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAAC GTTTGTTTTGAGTGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGG ATCTGCCTGGAGTGCTCGGGGAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGC TCTGTTACTATGGACAAGTGGAAGGACATTGAGCTTGAGAAGATGAAAGCTGGTGGGAAT GCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGATTACGATCCTTGCTGGTCCTTGCAG GAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTGGTCGCTCTGGCCGAA GGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAGCCCAGG ACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCG GACAAGGCTTTTGAAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGGGG AATCGCTACGTGGGGTTTGGGAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTCCTC AACAACGCCATGTCCTCCCTGTACTCGGGCTGGAGCAGCTTCACCACTGGAGCCAGCCGG TTTGCCTCGGCAGCCAAGGAGGGCGCTACAAAGTTTGGATCCCAAGCGAGTCAGAAGGCG TCCGAGCTGGGCCACAGCCTGAACGAGAACGTCCTCAAGCCTGCGCAGGAGAAGGTGAAG GAGGGAAAGATTTTTGATGATGTCTCCAGTGGGGTCTCTCAGTTGGCGTCCAAGGTCCAG GGAGTCGGTAGTAAGGGATGGCGGGACGTCACCACCTTTTTTTCGGGGAAAGCAGAGGGC CCCTTGGACAGCCCCTCGGAGGGCCACAGTTATCAGAACAGCGGTCTGGACCACTTCCAA AACAGCAACATAGACCAGAGCTTCTGGGAGACCTTTGGAAGTGCTGAGCCCACCAAGACC CGCAAGTCCCCGAGCAGCGACAGCTGGACGTGCGCGGACACCTCCACCGAGAGGAGGAGC TCGGACAGCTGGGAGGTGTGGGGCTCGGCCTCCACCAACAGGAACAGCAACAGCGACGGC GGGGAGGGCGGGGAGGGCACCAAGAAGGCAGTGCCGCCGGCCGTGCCCACTGATGATGGC TGGGACAACCAGAACTGGTAG Clone variation with respect to NM_018209.2 >OriGene 5' read for NM_018209 unedited
NCATTTACCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGGGGCGACCGGGCGCATCCTCATTGCAGTGCGGCGGCCCTACCTC GGCCCTGGCCTGACCCCGGCGGCCCTGCCCGCCCCTCCCTCCAGCATCATGGCCAGCCCA AGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAACGTTTGTTTTGAG TGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGGATCTGCCTGGAG TGCTCGGGGAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGCTCTGTTACTATG GACAAGTGGAAGGACATTGAGCTTGAGAAGATGAAAGCTGGTGGGAATGCTAAGTTCCGA GAGTTCCTGGAGTCTCAGGAGGATTACGATCCTTGCTGGTCCTTGCAGGAGAAGTACAAC AGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTGGTCGCTCTGGCCGAAGGCAGAGAGTGG TCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAGCCCAGGACGCTGCCGTCC ATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCGGACAGGCTTTTG AAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGNGGAATCGCTACGTGG GGGNTTGGNGAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTNCTCAACACGCCATG TCCCTCCTGTACTCGGNCTGGNAGCAGCTCCCACTGGAGCCCAGCCGGNTTGCTCGCAGC CAGGNAGGCGCTACAAAGTTGGATCCCAGCGATA >OriGene 3' read for NM_018209 unedited
CGGACCATAGGNGATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGGTCACAGGG NATGCCACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGT TTTTTTTTTTTTTTTTTGCATGTTCCCGTTTGCTTTATTGGAATGCTGTCAGGTCCGCGC CTTCCACCTGGGCCCTCACACACAGCAAGGAGAGGCCCCAGCACCGGTCCCAGGCCCACC TGCCCGCACCTGCAGAAGGGAAAGGCCATCANCCCTTCCGTGGACGGGGTCTCGGCCACC ACATCCACCTTCTGAAGGTGGCCCAGACACCTCCACGCTGCTGACTGCACTTCCCATCAA AAGGGACTCCCTGGGGCAGAGTGGGCCGTCCCCCTACCCCCGAGGAAGGCACCCTCCTGG GCGGGGACAGACCTTGGCTCCATGCTGCCAAGTCAAGGTGCTGGCGTGGAAACAGGCACA TGTGGAACACCACATCCCACTGTCCAAGGTGGAGTCCACCCCTCCTCGGAGCACTCAGCC CACCAGGGTCCAAGCAGCCCTCGGGAGATACCACGCGGCCGCCCACGTCTCAGCTTGGAG AAAGACACTCAAAGACCCCCACGCTCAGCTCTCGGGGGCTGGCTTCCTGGGCCCTTTGTG CTGGCTGCTTTCCCGGTGGCTTCTCAGCACCTTGAGCCTCTGATGCCCCGCCTCTGACCT CAGGTCCAACTACAGCAGGTGGCTGCTTCGGTGATCAAATCCCTCCGTGGCAGCCAAAGA CACCGGGCCCTCCACACCAGCTACCAAGAGGGCTGGTGCCCACAGGGAGGACCGGGNCGC CAGNAGGGCTCGCTGAAATCTTCGTCCCATCCTGGTCTGGCAGTGGCTGAATCCCCTAGG TGGGAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018209 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018209.2, NP_060679.1 |
| RefSeq Size | 3250 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 55738 |
| UniProt ID | Q8N6T3 |
| Domains | ArfGap |
| Protein Pathways | Endocytosis |
| Gene Summary | The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) lacks an in-frame exon and uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. The encoded isoform (a) is shorter, compared to isoform b. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Small-molecule synergist of the Wnt/{beta}-catenin signaling pathway
,Qisheng Zhang, Michael B Major, Shinichi Takanashi, Nathan D Camp, Naoyuki Nishiya, Eric C Peters, Mark H Ginsberg, Peter G Schultz, Randall T Moon, and Sheng Ding,
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7444-8.
[ARFGAP1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215268 | ARFGAP1 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1 |
CNY 3,656.00 |
|
| RC215268L1 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC215268L2 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC215268L3 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215268L4 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG215268 | ARFGAP1 (tGFP-tagged) - Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1 |
CNY 5,256.00 |
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